Determination of Rennin and Bovine Pepsins in Commercial Rennets and Abomasal Juices

• Published in: Journal of dairy science Vol. 55; no. 12; pp. 1641 - 1650
• Main Authors: Garnot, Pascaline, Thapon, J.L., Mathieu, C.M., Maubois, J.L., Dumas, B. Ribadeau
• Format: Journal Article
• Language: English
• Published: Champaign Elsevier Inc 01.12.1972; Am Dairy Sci Assoc; American Dairy Science Association
• Subjects: dairy products

A DEAE cellulose column chromatographic method is described to separate and to titrate rennin and bovine pepsin II from a mixture. Microgranular DEAE cellulose was equilibrated in .02m piper-azine buffer pH 5.3 and was placed on a 1×8cm column. The enzyme solution was poured on the column, as such,...