Determination of Rennin and Bovine Pepsins in Commercial Rennets and Abomasal Juices
A DEAE cellulose column chromatographic method is described to separate and to titrate rennin and bovine pepsin II from a mixture. Microgranular DEAE cellulose was equilibrated in .02m piper-azine buffer pH 5.3 and was placed on a 1×8cm column. The enzyme solution was poured on the column, as such,...
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| Published in | Journal of dairy science Vol. 55; no. 12; pp. 1641 - 1650 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Champaign
Elsevier Inc
01.12.1972
Am Dairy Sci Assoc American Dairy Science Association |
| Subjects | |
| Online Access | Get full text |
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| Abstract | A DEAE cellulose column chromatographic method is described to separate and to titrate rennin and bovine pepsin II from a mixture. Microgranular DEAE cellulose was equilibrated in .02m piper-azine buffer pH 5.3 and was placed on a 1×8cm column. The enzyme solution was poured on the column, as such, or, if necessary, after dialysis against the same buffer. Elution of enzymes was by a linear gradient of NaCl (100ml of .15m NaCl in the first chamber and 100ml of .5m NaCl in the second chamber). The flow was about 80ml per hour. Rennin was eluted in the first fraction and pepsin II in the second fraction. This method was used to determine the enzymatic composition of several commercial rennet preparations and abomasal juices collected from fistulated calves. |
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| AbstractList | A DEAE cellulose column chromatographic method is described to separate and to titrate rennin and bovine pepsin II from a mixture. Microgranular DEAE cellulose was equilibrated in .02m piper-azine buffer pH 5.3 and was placed on a 1×8cm column. The enzyme solution was poured on the column, as such, or, if necessary, after dialysis against the same buffer. Elution of enzymes was by a linear gradient of NaCl (100ml of .15m NaCl in the first chamber and 100ml of .5m NaCl in the second chamber). The flow was about 80ml per hour. Rennin was eluted in the first fraction and pepsin II in the second fraction. This method was used to determine the enzymatic composition of several commercial rennet preparations and abomasal juices collected from fistulated calves. |
| Author | Maubois, J.L. Mathieu, C.M. Garnot, Pascaline Thapon, J.L. Dumas, B. Ribadeau |
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| Cites_doi | 10.1017/S0022029900012796 10.1038/2051011a0 10.1016/0005-2744(68)90030-2 10.1016/S0300-9084(71)80161-X 10.1016/S0300-9084(71)80099-8 10.1042/bj0390179 10.1051/lait:197150825 10.1017/S0022029900003794 |
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| References_xml | – volume: 151 start-page: 719 year: 1968 ident: bib19 article-title: Colored inhibitors of pepsin publication-title: Biochim. Biophys. Acta contributor: fullname: Mal’tsen – volume: 13 start-page: 145 year: 1943 ident: bib2 article-title: The production of rennet from living calves publication-title: J. Dairy Res. contributor: fullname: Spratling – volume: 42 start-page: 250 year: 1965 ident: bib14 article-title: The operation for collecting rennet through an abomasal fistula publication-title: The Indian Veter. J. contributor: fullname: Bhalerao – volume: 51 start-page: 495 year: 1971 ident: bib13 article-title: Preparation de fromage à partir de “préfromage Iiquide” obtenu par ultrafiltration du lait publication-title: Lait contributor: fullname: Mocquot – volume: 23 start-page: 252 year: 1970 ident: bib15 article-title: Rennet from buffalo and cow fistulated calves publication-title: Indian J. 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Acta doi: 10.1016/0005-2744(68)90030-2 contributor: fullname: Stepanov – volume: 53 start-page: 447 year: 1971 ident: 10.3168/jds.S0022-0302(72)85740-0_bib7 article-title: Inactivation par l’urée de la présure et des pepsines bovines Application possible à leur dosage en mélange publication-title: Biochimie doi: 10.1016/S0300-9084(71)80161-X contributor: fullname: Douillard – volume: 53 start-page: 321 year: 1971 ident: 10.3168/jds.S0022-0302(72)85740-0_bib1 article-title: Isolemerit, purification et propriétés de deux zymogénes gastriques bovins Propriétés de proteases correspondantes publication-title: Biochimie doi: 10.1016/S0300-9084(71)80099-8 contributor: fullname: Antonini – year: 1971 ident: 10.3168/jds.S0022-0302(72)85740-0_bib12 article-title: Rep. Annual Session Int. Dairy Federation. Dublin. Commission publication-title: B. Technol. Eng contributor: fullname: de Koning – volume: 42 start-page: 250 year: 1965 ident: 10.3168/jds.S0022-0302(72)85740-0_bib14 article-title: The operation for collecting rennet through an abomasal fistula publication-title: The Indian Veter. J. contributor: fullname: Nair – volume: 39 start-page: 179 year: 1945 ident: 10.3168/jds.S0022-0302(72)85740-0_bib3 article-title: The purification and cristallisation of rennin publication-title: Biochem. J. doi: 10.1042/bj0390179 contributor: fullname: Berridge – volume: 51 start-page: 495 year: 1971 ident: 10.3168/jds.S0022-0302(72)85740-0_bib13 article-title: Preparation de fromage à partir de “préfromage Iiquide” obtenu par ultrafiltration du lait publication-title: Lait doi: 10.1051/lait:197150825 contributor: fullname: Maubois – volume: 52 start-page: 8 issue: 5 year: 1961 ident: 10.3168/jds.S0022-0302(72)85740-0_bib8 article-title: Milk clotting activity of pepsin and rennin publication-title: Milk Prod. J. contributor: fullname: Ernstrom – ident: 10.3168/jds.S0022-0302(72)85740-0_bib18 – volume: 48 start-page: 969 year: 1966 ident: 10.3168/jds.S0022-0302(72)85740-0_bib20 article-title: Méthode d’é1ectrophorése dans les gels d’acrylamide-agarose publication-title: Bull. Soc. Chim. Biol. contributor: fullname: Uriel – volume: 52 start-page: 1429 year: 1970 ident: 10.3168/jds.S0022-0302(72)85740-0_bib6 article-title: Détermination avec la caséine kappa de 1’activité protéolytique de la présure, de la pepsine de pore et des pepsines bovines publication-title: Bull. Soc. Chim. Biol contributor: fullname: Douillard – volume: 13 start-page: 145 year: 1943 ident: 10.3168/jds.S0022-0302(72)85740-0_bib2 article-title: The production of rennet from living calves publication-title: J. Dairy Res. doi: 10.1017/S0022029900003794 contributor: fullname: Berridge – volume: 10 start-page: 358 year: 1971 ident: 10.3168/jds.S0022-0302(72)85740-0_bib9 article-title: Bovine pepsin: preliminary cheesemaking experiments publication-title: Irish. J. Agr. Res. contributor: fullname: Fox – volume: 23 start-page: 252 year: 1970 ident: 10.3168/jds.S0022-0302(72)85740-0_bib15 article-title: Rennet from buffalo and cow fistulated calves publication-title: Indian J. Dairy Sci. contributor: fullname: Mathur |
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| Snippet | A DEAE cellulose column chromatographic method is described to separate and to titrate rennin and bovine pepsin II from a mixture. Microgranular DEAE cellulose... |
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| Title | Determination of Rennin and Bovine Pepsins in Commercial Rennets and Abomasal Juices |
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