A noncoding RNA produced by arthropod-borne flaviviruses inhibits the cellular exoribonuclease XRN1 and alters host mRNA stability

• Published in: RNA (Cambridge) Vol. 18; no. 11; pp. 2029 - 2040
• Main Authors: Moon, Stephanie L, Anderson, John R, Kumagai, Yutaro, Wilusz, Carol J, Akira, Shizuo, Khromykh, Alexander A, Wilusz, Jeffrey
• Format: Journal Article
• Language: English
• Published: United States Cold Spring Harbor Laboratory Press 01.11.2012
• Subjects: 3' Untranslated Regions; Aedes - cytology; Aedes - virology; Animals; Cell Line; Cricetinae; Dengue Virus - genetics; Dengue Virus - physiology; Exoribonucleases - antagonists & inhibitors; Exoribonucleases - chemistry; Exoribonucleases - metabolism; Gene Expression Regulation; Half-Life; Host-Pathogen Interactions; Humans; Insect Proteins - antagonists & inhibitors; Insect Proteins - chemistry; Insect Proteins - metabolism; Microtubule-Associated Proteins - antagonists & inhibitors; Microtubule-Associated Proteins - chemistry; Microtubule-Associated Proteins - metabolism; Oligonucleotide Array Sequence Analysis; RNA Stability; RNA, Messenger - chemistry; RNA, Messenger - metabolism; RNA, Untranslated - chemistry; RNA, Untranslated - metabolism; RNA, Untranslated - physiology; RNA, Viral - chemistry; RNA, Viral - metabolism; RNA, Viral - physiology; Saccharomyces cerevisiae Proteins - antagonists & inhibitors; Saccharomyces cerevisiae Proteins - chemistry; Transcriptome; West Nile virus - genetics; West Nile virus - physiology

All arthropod-borne flaviviruses generate a short noncoding RNA (sfRNA) from the viral 3' untranslated region during infection due to stalling of the cellular 5'-to-3' exonuclease XRN1. We show here that formation of sfRNA also inhibits XRN1 activity. Cells infected with Dengue or Kun...