Use of an inducible promoter for antibiotic production in a heterologous host

• Published in: Applied microbiology and biotechnology Vol. 87; no. 1; pp. 261 - 269
• Main Authors: Dangel, Volker, Westrich, Lucia, Smith, Margaret C. M, Heide, Lutz, Gust, Bertolt
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.06.2010; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Anti-Bacterial Agents - biosynthesis; Antibiotics; Applied Genetics and Molecular Biotechnology; Binding sites; Biological and medical sciences; Biomedical and Life Sciences; Biosynthesis; Biotechnology; Chemical reactions; E coli; Fundamental and applied biological sciences. Psychology; Genes; Genetic Engineering; Inducible promoter; Life Sciences; Medical research; Microbial Genetics and Genomics; Microbiology; novobiocin; Novobiocin - biosynthesis; Plasmids; Promoter Regions, Genetic; Ribonucleic acid; RNA; Streptococcus infections; Streptomyces; Streptomyces coelicolor; Streptomyces coelicolor - genetics; Streptomyces coelicolor - metabolism; Studies; Streptomyces; Genetic engineering; Novobiocin; Inducible promoter; Actinomycetales; Antibiotic; Streptomycetaceae; Production; Bacteria; Actinomycetes

The biosynthetic gene cluster of the aminocoumarin antibiotic novobiocin comprises 20 coding sequences. Sixteen of them code for essential enzymes for novobiocin formation, transcribed in the form of a single 18-kb polycistronic mRNA. In the present study, we replaced the genuine promoter of this op...