Menstrual effluent induces epithelial–mesenchymal transitions in mesothelial cells

• Published in: Human reproduction (Oxford) Vol. 19; no. 1; pp. 21 - 29
• Main Authors: Demir, A.Y., Groothuis, P.G., Nap, A.W., Punyadeera, C., Goeij, A.F.P.M.de, Evers, J.L.H., Dunselman, G.A.J.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.01.2004; Oxford Publishing Limited (England)
• Subjects: Actins - metabolism; Biological and medical sciences; Cadherins - genetics; Cadherins - metabolism; Cell Communication; Cell Movement; Cells, Cultured; Culture Media, Conditioned - pharmacology; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Embryology: invertebrates and vertebrates. Teratology; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Epithelial Cells - pathology; E‐cadherin/endometriosis/mesothelial cells/Snail/vimentin; Female; Fundamental and applied biological sciences. Psychology; Genistein - pharmacology; Humans; Indoles - pharmacology; Keratins - metabolism; Menstruation; Omentum - drug effects; Omentum - pathology; Omentum - physiopathology; Phenotype; RNA, Messenger - metabolism; Snail Family Transcription Factors; Sodium Azide - pharmacology; Sulfonamides - pharmacology; Tissue Distribution; Transcription Factors - genetics; Transcription Factors - metabolism; Tubulin - metabolism; Up-Regulation; Vimentin - genetics; Vimentin - metabolism; E‐cadherin/endometriosis/mesothelial cells/Snail/vimentin; Epithelial cell; Vertebrata; E-cadherin/endometriosis/mesothelial cells/Snail/vimentin; Mammalia

BACKGROUND: Menstrual effluent affects mesothelial cell (MC) morphology. We evaluated whether these changes were consistent with epithelial–mesenchymal transitions (EMT). METHODS: Monolayer cultures of MC were incubated overnight in conditioned media, prepared from cells isolated form menstrual effl...