Molecular Characterization of the Dot/Icm-Translocated AnkH and AnkJ Eukaryotic-Like Effectors of Legionella pneumophila
Although most Dot/Icm-translocated effectors of Legionella pneumophila are not required for intracellular proliferation, the eukaryotic-like ankyrin effectors, AnkH and AnkJ are required for intracellular proliferation. In this report, we show that the IcmSW chaperones are essential for translocatio...
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Published in | Infection and Immunity Vol. 78; no. 3; pp. 1123 - 1134 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
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Washington, DC
American Society for Microbiology
01.03.2010
American Society for Microbiology (ASM) |
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Abstract | Although most Dot/Icm-translocated effectors of Legionella pneumophila are not required for intracellular proliferation, the eukaryotic-like ankyrin effectors, AnkH and AnkJ are required for intracellular proliferation. In this report, we show that the IcmSW chaperones are essential for translocation of AnkJ but not AnkH. The 10 C-terminal residues and the ANK domains of AnkH and AnkJ are required for translocation. Our data indicate that the two ANK domains of AnkH are critical domains required for the function of the effector in intracellular replication of L. pneumophila. The ankH and ankJ mutants are severely defective in intrapulmonary proliferation in mice. Expression of AnkH and AnkJ fusions within HEK293 cells show a punctuate distribution in the cytosol but no association with endocytic vesicles, the Golgi apparatus or the endoplasmic reticulum. Interestingly, the defect in intracellular proliferation of the ankH or ankJ mutants is rescued in HEK293 cells expressing the respective protein. We conclude that AnkH and AnkJ are effectors translocated by the Dot/Icm system by distinct mechanisms and modulate distinct cytosolic processes in the host cell. |
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AbstractList | Although most Dot/Icm-translocated effectors of Legionella pneumophila are not required for intracellular proliferation, the eukaryotic-like ankyrin effectors, AnkH and AnkJ are required for intracellular proliferation. In this report, we show that the IcmSW chaperones are essential for translocation of AnkJ but not AnkH. The 10 C-terminal residues and the ANK domains of AnkH and AnkJ are required for translocation. Our data indicate that the two ANK domains of AnkH are critical domains required for the function of the effector in intracellular replication of L. pneumophila. The ankH and ankJ mutants are severely defective in intrapulmonary proliferation in mice. Expression of AnkH and AnkJ fusions within HEK293 cells show a punctuate distribution in the cytosol but no association with endocytic vesicles, the Golgi apparatus or the endoplasmic reticulum. Interestingly, the defect in intracellular proliferation of the ankH or ankJ mutants is rescued in HEK293 cells expressing the respective protein. We conclude that AnkH and AnkJ are effectors translocated by the Dot/Icm system by distinct mechanisms and modulate distinct cytosolic processes in the host cell. Although most Dot/Icm-translocated effectors of Legionella pneumophila are not required for intracellular proliferation, the eukaryotic-like ankyrin effectors, AnkH and AnkJ are required for intracellular proliferation. In this report, we show that the IcmSW chaperones are essential for translocation of AnkJ but not AnkH. The 10 C-terminal residues and the ANK domains of AnkH and AnkJ are required for translocation. Our data indicate that the two ANK domains of AnkH are critical domains required for the function of the effector in intracellular replication of L. pneumophila . The ankH and ankJ mutants are severely defective in intrapulmonary proliferation in mice. Expression of AnkH and AnkJ fusions within HEK293 cells show a punctuate distribution in the cytosol but no association with endocytic vesicles, the Golgi apparatus or the endoplasmic reticulum. Interestingly, the defect in intracellular proliferation of the ankH or ankJ mutants is rescued in HEK293 cells expressing the respective protein. We conclude that AnkH and AnkJ are effectors translocated by the Dot/Icm system by distinct mechanisms and modulate distinct cytosolic processes in the host cell. Classifications Services IAI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue IAI About IAI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy Connect to IAI IAI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0019-9567 Online ISSN: 1098-5522 Copyright © 2014 by the American Society for Microbiology. For an alternate route to IAI .asm.org, visit: IAI |
Author | Habyarimana, Fabien Kwaik, Yousef Abu Santic, Marina Al-Khodor, Souhaila Price, Chris T |
AuthorAffiliation | Department of Microbiology and Immunology, Room MS-410, College of Medicine, University of Louisville, Louisville, Kentucky 40292 |
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Cites_doi | 10.1006/mpat.1998.0237 10.1128/AEM.64.9.3127-3133.1998 10.1371/journal.ppat.1000704 10.1038/ncb883 10.1128/IAI.73.5.3166-3171.2005 10.1111/j.1365-2958.2008.06124.x 10.1056/NEJM197712012972201 10.1016/S0962-8924(00)88956-4 10.1084/jem.158.6.2108 10.1111/j.1462-5822.2006.00871.x 10.1128/JB.187.22.7716-7726.2005 10.1128/JB.01656-08 10.1371/journal.ppat.0020046 10.1242/jcs.114.24.4637 10.1073/pnas.0609012103 10.1073/pnas.0304916101 10.1111/j.1462-5822.2008.01219.x 10.1074/jbc.M501746200 10.1038/nrmicro1967 10.1111/j.1365-2958.2005.04539.x 10.1128/IAI.01278-06 10.1126/science.279.5353.1037 10.1038/ng1447 10.1016/j.mib.2008.12.004 10.1128/IAI.00023-09 10.1084/jem.20031706 10.1073/pnas.0406239101 10.1126/science.1158160 10.1371/journal.ppat.0030188 10.1110/ps.03554604 10.1111/j.1462-2920.2007.01560.x 10.1371/journal.ppat.1000117 10.1111/j.1365-2958.2004.04435.x 10.1111/j.1365-2958.2008.06453.x 10.1128/IAI.70.3.1657-1663.2002 10.1128/jcm.10.4.437-441.1979 10.1093/oxfordjournals.aje.a113200 10.1111/j.1462-5822.2007.00899.x 10.1128/IAI.72.5.3048-3053.2004 10.1007/BF01570838 |
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References | (e_1_3_1_39_2) 1999; 12 (e_1_3_1_10_2) 1994; 145 e_1_3_1_21_2 e_1_3_1_43_2 e_1_3_1_22_2 e_1_3_1_44_2 e_1_3_1_23_2 e_1_3_1_45_2 e_1_3_1_46_2 e_1_3_1_24_2 e_1_3_1_8_2 e_1_3_1_7_2 e_1_3_1_40_2 e_1_3_1_41_2 e_1_3_1_9_2 e_1_3_1_20_2 e_1_3_1_42_2 e_1_3_1_4_2 e_1_3_1_29_2 e_1_3_1_3_2 e_1_3_1_6_2 e_1_3_1_25_2 e_1_3_1_26_2 e_1_3_1_47_2 e_1_3_1_2_2 e_1_3_1_27_2 e_1_3_1_28_2 (e_1_3_1_5_2) 2008; 70 e_1_3_1_32_2 e_1_3_1_33_2 e_1_3_1_34_2 e_1_3_1_35_2 e_1_3_1_13_2 e_1_3_1_12_2 e_1_3_1_11_2 e_1_3_1_30_2 e_1_3_1_31_2 e_1_3_1_17_2 e_1_3_1_16_2 e_1_3_1_15_2 e_1_3_1_14_2 e_1_3_1_36_2 (e_1_3_1_37_2) 2005; 55 e_1_3_1_19_2 e_1_3_1_38_2 e_1_3_1_18_2 |
References_xml | – ident: e_1_3_1_21_2 doi: 10.1006/mpat.1998.0237 – ident: e_1_3_1_3_2 doi: 10.1128/AEM.64.9.3127-3133.1998 – ident: e_1_3_1_40_2 doi: 10.1371/journal.ppat.1000704 – ident: e_1_3_1_4_2 – volume: 145 start-page: 1537 year: 1994 ident: e_1_3_1_10_2 publication-title: Am. J. Pathol. – ident: e_1_3_1_28_2 doi: 10.1038/ncb883 – ident: e_1_3_1_42_2 doi: 10.1128/IAI.73.5.3166-3171.2005 – ident: e_1_3_1_31_2 doi: 10.1111/j.1365-2958.2008.06124.x – ident: e_1_3_1_20_2 doi: 10.1056/NEJM197712012972201 – ident: e_1_3_1_43_2 doi: 10.1016/S0962-8924(00)88956-4 – ident: e_1_3_1_24_2 doi: 10.1084/jem.158.6.2108 – ident: e_1_3_1_25_2 doi: 10.1111/j.1462-5822.2006.00871.x – ident: e_1_3_1_15_2 doi: 10.1128/JB.187.22.7716-7726.2005 – ident: e_1_3_1_45_2 doi: 10.1128/JB.01656-08 – ident: e_1_3_1_46_2 doi: 10.1371/journal.ppat.0020046 – ident: e_1_3_1_44_2 doi: 10.1242/jcs.114.24.4637 – ident: e_1_3_1_32_2 doi: 10.1073/pnas.0609012103 – ident: e_1_3_1_34_2 doi: 10.1073/pnas.0304916101 – ident: e_1_3_1_41_2 doi: 10.1111/j.1462-5822.2008.01219.x – ident: e_1_3_1_6_2 doi: 10.1074/jbc.M501746200 – ident: e_1_3_1_27_2 doi: 10.1038/nrmicro1967 – ident: e_1_3_1_8_2 doi: 10.1111/j.1365-2958.2005.04539.x – ident: e_1_3_1_33_2 doi: 10.1128/IAI.01278-06 – ident: e_1_3_1_7_2 – ident: e_1_3_1_9_2 doi: 10.1126/science.279.5353.1037 – ident: e_1_3_1_12_2 doi: 10.1038/ng1447 – ident: e_1_3_1_17_2 doi: 10.1016/j.mib.2008.12.004 – ident: e_1_3_1_22_2 doi: 10.1128/IAI.00023-09 – ident: e_1_3_1_29_2 doi: 10.1084/jem.20031706 – ident: e_1_3_1_36_2 doi: 10.1073/pnas.0406239101 – volume: 12 start-page: 29 year: 1999 ident: e_1_3_1_39_2 publication-title: Vestn. Ross Akad. Med. Nauk. – ident: e_1_3_1_38_2 doi: 10.1126/science.1158160 – ident: e_1_3_1_11_2 doi: 10.1371/journal.ppat.0030188 – ident: e_1_3_1_26_2 doi: 10.1111/j.1462-5822.2006.00871.x – ident: e_1_3_1_35_2 doi: 10.1110/ps.03554604 – ident: e_1_3_1_23_2 doi: 10.1111/j.1462-2920.2007.01560.x – ident: e_1_3_1_14_2 doi: 10.1371/journal.ppat.1000117 – volume: 55 start-page: 912 year: 2005 ident: e_1_3_1_37_2 publication-title: Mol. Microbiol. doi: 10.1111/j.1365-2958.2004.04435.x – volume: 70 start-page: 908 year: 2008 ident: e_1_3_1_5_2 publication-title: Mol. Microbiol. doi: 10.1111/j.1365-2958.2008.06453.x – ident: e_1_3_1_47_2 doi: 10.1128/IAI.70.3.1657-1663.2002 – ident: e_1_3_1_18_2 doi: 10.1128/jcm.10.4.437-441.1979 – ident: e_1_3_1_30_2 doi: 10.1093/oxfordjournals.aje.a113200 – ident: e_1_3_1_2_2 – ident: e_1_3_1_13_2 doi: 10.1111/j.1462-5822.2007.00899.x – ident: e_1_3_1_16_2 doi: 10.1128/IAI.72.5.3048-3053.2004 – ident: e_1_3_1_19_2 doi: 10.1007/BF01570838 |
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Snippet | Although most Dot/Icm-translocated effectors of Legionella pneumophila are not required for intracellular proliferation, the eukaryotic-like ankyrin effectors,... Classifications Services IAI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit... Although most Dot/Icm-translocated effectors of Legionella pneumophila are not required for intracellular proliferation, the eukaryotic-like ankyrin effectors,... |
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SubjectTerms | Animals Ankyrins - genetics Ankyrins - physiology Bacteriology Biological and medical sciences Cell Line Colony Count, Microbial Cytoplasm - chemistry Disease Models, Animal Fundamental and applied biological sciences. Psychology Humans Legionella pneumophila Legionella pneumophila - pathogenicity Legionnaires' Disease - microbiology Mice Microbiology Miscellaneous Molecular Chaperones - genetics Molecular Chaperones - metabolism Molecular Pathogenesis Protein Interaction Domains and Motifs Protein Interaction Mapping Survival Analysis Virulence Factors - genetics Virulence Factors - physiology |
Title | Molecular Characterization of the Dot/Icm-Translocated AnkH and AnkJ Eukaryotic-Like Effectors of Legionella pneumophila |
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