miR-489 is a tumour-suppressive miRNA target PTPN11 in hypopharyngeal squamous cell carcinoma (HSCC)
Background: Hypopharyngeal squamous cell carcinoma (HSCC) is an aggressive malignancy with one of the worst prognoses among all head and neck cancers. Greater understanding of the pertinent molecular oncogenic pathways could help improve diagnosis, therapy, and prevention of this disease. The aim of...
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Published in | British journal of cancer Vol. 103; no. 6; pp. 877 - 884 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
07.09.2010
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | Background:
Hypopharyngeal squamous cell carcinoma (HSCC) is an aggressive malignancy with one of the worst prognoses among all head and neck cancers. Greater understanding of the pertinent molecular oncogenic pathways could help improve diagnosis, therapy, and prevention of this disease. The aim of this study was to identify tumour-suppressive microRNAs (miRNAs), based on miRNA expression signatures from clinical HSCC specimens, and to predict their biological target genes.
Methods:
Expression levels of 365 human mature miRNAs from 10 HSCC clinical samples were screened using stem-loop real-time quantitative PCR. Downregulated miRNAs were used in cell proliferation assays to identify a tumour-suppressive miRNA. Genome-wide gene expression analyses were then performed to identify the target genes of the tumour-suppressive miRNA.
Results:
Expression analysis identified 11 upregulated and 31 downregulated miRNAs. Gain-of-function analysis of the downregulated miRNAs revealed that
miR-489
inhibited cell growth in all head and neck cancer cell lines examined. The gene
PTPN11
coding for a cytoplasmic protein tyrosine phosphatase containing two Src Homology 2 domains was identified as a
miR-489
-targeted gene. Knockdown of
PTPN11
resulted in the inhibition of cell proliferation in head and neck SCC cells.
Conclusion:
Identification of the tumour-suppressive miRNA
miR-489
and its target,
PTPN11
, might provide new insights into the underlying molecular mechanisms of HSCC. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0007-0920 1532-1827 |
DOI: | 10.1038/sj.bjc.6605811 |