Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 109; no. 3; pp. 661 - 662
• Main Authors: Rego, E. Hesper, Shao, Lin, Macklin, John J., Winoto, Lukman, Johansson, Göran A., Kamps-Hughes, Nicholas, Davidson, Michael W., Gustafsson, Mats G. L.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 17.01.2012; National Acad Sciences
• Series: PNAS Plus
• Subjects: Actin Cytoskeleton - metabolism; Animals; Biological samples; Biological Sciences; Cells; Cells - metabolism; CHO Cells; Cricetinae; Cricetulus; Cytoskeleton; Fluorescence; HEK293 Cells; Humans; image analysis; Light; light intensity; light microscopes; Luminescent Proteins - metabolism; Mammals; microfilaments; Microscopy; Microscopy - methods; microtubules; Microtubules - metabolism; Nonlinear Dynamics; nuclear membrane; Nuclear Pore - metabolism; Photobleaching; PNAS Plus; PNAS PLUS (AUTHOR SUMMARIES); Polystyrene; Proteins

Using ultralow light intensities that are well suited for investigating biological samples, we demonstrate whole-cell superresolution imaging by nonlinear structured-illumination microscopy. Structured-illumination microscopy can increase the spatial resolution of a wide-field light microscope by a...