Utilisation of bacteriophage display libraries to identify peptide sequences recognised by Equine herpesvirus type 1 specific equine sera

• Published in: Journal of virological methods Vol. 88; no. 1; pp. 89 - 104
• Main Authors: Birch-Machin, I, Ryder, S, Taylor, L, Iniguez, P, Marault, M, Ceglie, L, Zientara, S, Cruciere, C, Cancellotti, F, Koptopoulos, G, Mumford, J, Binns, M, Davis-Poynter, N, Hannant, D
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 01.07.2000; Amsterdam Elsevier; New York, NY
• Subjects: Amino Acid Sequence; Animals; Antibodies, Viral - blood; Antibodies, Viral - immunology; Antigens, Viral - chemistry; Antigens, Viral - genetics; Antigens, Viral - immunology; Bacteriophages - genetics; Biological and medical sciences; EHV-1; ELISAs; Enzyme-Linked Immunosorbent Assay; Epitope Mapping; Equine herpesvirus 1; Fundamental and applied biological sciences. Psychology; Herpesviridae Infections - immunology; Herpesviridae Infections - veterinary; Herpesviridae Infections - virology; Herpesvirus 1, Equid - genetics; Herpesvirus 1, Equid - immunology; Horse Diseases - immunology; Horse Diseases - virology; Horses; Life Sciences; Microbiology; Mimotopes; Molecular Sequence Data; Peptide Library; Peptides - chemistry; Peptides - genetics; Peptides - immunology; Phage display libraries; Sensitivity and Specificity; Sequence Analysis, DNA; Techniques used in virology; Virology; Epitope mapping; EHV-1; Phage display libraries; ELISAs; Mimotopes; Equine herpesvirus 1; Antigenic determinant; Peptides; Herpesviridae; Alphaherpesvirinae; Method; Enzyme immunoassay; Virus; Characterization; Vertebrata; Phage display; Mammalia; Horse; Perissodactyla; Veterinary; ELISA assay; Ungulata

Three filamentous phage random peptide display libraries were used in biopanning experiments with purified IgG from the serum of a gnotobiotic foal infected with equine herpesvirus-1 (EHV-1) to enrich for epitopes binding to anti-EHV-1 antibodies. The sequences of the amino acids displayed were alig...