Identification and utilization of a 1,3-propanediol oxidoreductase isoenzyme for production of 1,3-propanediol from glycerol in Klebsiella pneumoniae

• Published in: Applied microbiology and biotechnology Vol. 85; no. 3; pp. 659 - 666
• Main Authors: Seo, Jeong-Woo, Seo, Mi-Young, Oh, Baek-Rock, Heo, Sun-Yeon, Baek, Jin-Oh, Rairakhwada, Dina, Luo, Lian Hua, Hong, Won-Kyung, Kim, Chul Ho
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 2010; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Alcohol Dehydrogenase; Alcohol Oxidoreductases - genetics; Alcohol Oxidoreductases - metabolism; Amino acids; Applied Genetics and Molecular Biotechnology; Bacteria; Biological and medical sciences; Biomedical and Life Sciences; Biosynthetic Pathways - genetics; Biotechnology; By products; Dehydrogenases; E coli; Enzymes; Escherichia coli; Escherichia coli - genetics; Fermentation; Fundamental and applied biological sciences. Psychology; Gene Deletion; Gene Dosage; Gene Expression; Genetic Complementation Test; Glycerol; Glycerol - metabolism; Isoenzymes - genetics; Isoenzymes - metabolism; Kinases; Klebsiella pneumoniae; Klebsiella pneumoniae - genetics; Klebsiella pneumoniae - metabolism; Life Sciences; Metabolism; Microbial Genetics and Genomics; Microbiology; Mutation; Plasmids; Pneumonia; Polymerization; Propylene Glycols - metabolism; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Studies; Textile fibers; 1,3-Propanediol; 1,3-Propanediol oxidoreductase isoenzyme; Glycerol metabolism; Enzyme; Isozyme; Glycerol; Identification; Metabolism; Production; Bacteria; Oxidoreductases; Propanediol; Klebsiella pneumoniae; Enterobacteriaceae

In a previous study, we showed that 1,3-propanediol (1,3-PD) was still produced from glycerol by the Klebsiella pneumoniae mutant strain defective in 1,3-PD oxidoreductase (DhaT), although the production level was lower compared to the parent strain. As a potential candidate for another putative 1,3...