Motility of efferent duct cilia aids passage of sperm cells through the male reproductive system

Motile cilia line the efferent ducts of the mammalian male reproductive tract. Several recent mouse studies have demonstrated that a reduced generation of multiple motile cilia in efferent ducts is associated with obstructive oligozoospermia and fertility issues. However, the sole impact of efferent...

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Published inMolecular human reproduction Vol. 27; no. 3
Main Authors Aprea, Isabella, Nöthe-Menchen, Tabea, Dougherty, Gerard W, Raidt, Johanna, Loges, Niki T, Kaiser, Thomas, Wallmeier, Julia, Olbrich, Heike, Strünker, Timo, Kliesch, Sabine, Pennekamp, Petra, Omran, Heymut
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LanguageEnglish
Published England Oxford University Press 27.02.2021
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Abstract Motile cilia line the efferent ducts of the mammalian male reproductive tract. Several recent mouse studies have demonstrated that a reduced generation of multiple motile cilia in efferent ducts is associated with obstructive oligozoospermia and fertility issues. However, the sole impact of efferent duct cilia dysmotility on male infertility has not been studied so far either in mice or human. Using video microscopy, histological- and ultrastructural analyses, we examined male reproductive tracts of mice deficient for the axonemal motor protein DNAH5: this defect exclusively disrupts the outer dynein arm (ODA) composition of motile cilia but not the ODA composition and motility of sperm flagella. These mice have immotile efferent duct cilia that lack ODAs, which are essential for ciliary beat generation. Furthermore, they show accumulation of sperm in the efferent duct. Notably, the ultrastructure and motility of sperm from these males are unaffected. Likewise, human individuals with loss-of-function DNAH5 mutations present with reduced sperm count in the ejaculate (oligozoospermia) and dilatations of the epididymal head but normal sperm motility, similar to DNAH5 deficient mice. The findings of this translational study demonstrate, in both mice and men, that efferent duct ciliary motility is important for male reproductive fitness and uncovers a novel pathomechanism distinct from primary defects of sperm motility (asthenozoospermia). If future work can identify environmental factors or defects in genes other than DNAH5 that cause efferent duct cilia dysmotility, this will help unravel other causes of oligozoospermia and may influence future practices in genetic and fertility counseling as well as ART.
AbstractList Motile cilia line the efferent ducts of the mammalian male reproductive tract. Several recent mouse studies have demonstrated that a reduced generation of multiple motile cilia in efferent ducts is associated with obstructive oligozoospermia and fertility issues. However, the sole impact of efferent duct cilia dysmotility on male infertility has not been studied so far either in mice or human. Using video microscopy, histological- and ultrastructural analyses, we examined male reproductive tracts of mice deficient for the axonemal motor protein DNAH5: this defect exclusively disrupts the outer dynein arm (ODA) composition of motile cilia but not the ODA composition and motility of sperm flagella. These mice have immotile efferent duct cilia that lack ODAs, which are essential for ciliary beat generation. Furthermore, they show accumulation of sperm in the efferent duct. Notably, the ultrastructure and motility of sperm from these males are unaffected. Likewise, human individuals with loss-of-function DNAH5 mutations present with reduced sperm count in the ejaculate (oligozoospermia) and dilatations of the epididymal head but normal sperm motility, similar to DNAH5 deficient mice. The findings of this translational study demonstrate, in both mice and men, that efferent duct ciliary motility is important for male reproductive fitness and uncovers a novel pathomechanism distinct from primary defects of sperm motility (asthenozoospermia). If future work can identify environmental factors or defects in genes other than DNAH5 that cause efferent duct cilia dysmotility, this will help unravel other causes of oligozoospermia and may influence future practices in genetic and fertility counseling as well as ART.
Motile cilia line the efferent ducts of the mammalian male reproductive tract. Several recent mouse studies have demonstrated that a reduced generation of multiple motile cilia in efferent ducts is associated with obstructive oligozoospermia and fertility issues. However, the sole impact of efferent duct cilia dysmotility on male infertility has not been studied so far either in mice or human. Using video microscopy, histological- and ultrastructural analyses, we examined male reproductive tracts of mice deficient for the axonemal motor protein DNAH5: this defect exclusively disrupts the outer dynein arm (ODA) composition of motile cilia but not the ODA composition and motility of sperm flagella. These mice have immotile efferent duct cilia that lack ODAs, which are essential for ciliary beat generation. Furthermore, they show accumulation of sperm in the efferent duct. Notably, the ultrastructure and motility of sperm from these males are unaffected. Likewise, human individuals with loss-of-function DNAH5 mutations present with reduced sperm count in the ejaculate (oligozoospermia) and dilatations of the epididymal head but normal sperm motility, similar to DNAH5 deficient mice. The findings of this translational study demonstrate, in both mice and men, that efferent duct ciliary motility is important for male reproductive fitness and uncovers a novel pathomechanism distinct from primary defects of sperm motility (asthenozoospermia). If future work can identify environmental factors or defects in genes other than DNAH5 that cause efferent duct cilia dysmotility, this will help unravel other causes of oligozoospermia and may influence future practices in genetic and fertility counseling as well as ART.
Author Omran, Heymut
Dougherty, Gerard W
Raidt, Johanna
Loges, Niki T
Kliesch, Sabine
Aprea, Isabella
Olbrich, Heike
Wallmeier, Julia
Strünker, Timo
Pennekamp, Petra
Nöthe-Menchen, Tabea
Kaiser, Thomas
AuthorAffiliation 2 Department of Clinical Andrology, Centre of Reproductive Medicine and Andrology, University Hospital Muenster , Muenster 48149, Germany
1 Department of General Pediatrics, University Hospital Muenster , Muenster 48149, Germany
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Keywords efferent ducts / cilia / sperm / motor proteins / outer dynein arm-defects / DNAH5 / 45 male infertility / oligozoospermia / motile ciliopathy / primary ciliary dyskinesia
Language English
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Petra Pennekamp and Heymut Omran share the last authorship.
Isabella Aprea and Tabea Nöthe-Menchen should be regarded as joint First Authors.
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Snippet Motile cilia line the efferent ducts of the mammalian male reproductive tract. Several recent mouse studies have demonstrated that a reduced generation of...
SourceID pubmedcentral
proquest
crossref
pubmed
SourceType Open Access Repository
Aggregation Database
Index Database
SubjectTerms Animals
Axonemal Dyneins - genetics
Axonemal Dyneins - metabolism
Axoneme - genetics
Axoneme - metabolism
Axoneme - ultrastructure
Cilia - genetics
Cilia - metabolism
Cilia - ultrastructure
Ciliary Motility Disorders - genetics
Ciliary Motility Disorders - metabolism
Ciliary Motility Disorders - pathology
Genetic Predisposition to Disease
Genitalia, Male - metabolism
Genitalia, Male - ultrastructure
Humans
Male
Mice
Mice, 129 Strain
Mice, Inbred C57BL
Mice, Transgenic
Movement
Mutation
Oligospermia - genetics
Oligospermia - metabolism
Oligospermia - pathology
Original Research
Phenotype
Sperm Motility
Spermatozoa - pathology
Spermatozoa - ultrastructure
Title Motility of efferent duct cilia aids passage of sperm cells through the male reproductive system
URI https://www.ncbi.nlm.nih.gov/pubmed/33561200
https://search.proquest.com/docview/2488191019
https://pubmed.ncbi.nlm.nih.gov/PMC7936721
Volume 27
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