Evaluation of kinetic parameters of immobilized penicillin G acylase subject to an inactivation and reactivation process

• Published in: Journal of molecular catalysis. B, Enzymatic Vol. 104; pp. 70 - 74
• Main Authors: Romero, Oscar, Araya, Erick, Illanes, Andrés, Wilson, Lorena
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.06.2014; Elsevier
• Subjects: benzylpenicillin; biocatalysts; Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; catalytic activity; dioxane; enzyme inactivation; Escherichia coli; Fundamental and applied biological sciences. Psychology; hydrolysis; Inactivaction; Kinetic parameters; longevity; Methods. Procedures. Technologies; Penicillin acylase; phenylacetic acid; Reactivation; solvents; Reactivation; Penicillin acylase; Kinetic parameters; Inactivaction; Penicillin amidase; Enzyme; Hydrolases; Kinetic parameter; Inactivation; Operating process

•Immobilized PGA was inactivated by organic solvent and reactivated by two strategies.•Apparent and intrinsic kinetic parameters were calculated in three biocatalyst states.•Catalyst inactivation and reactivation significantly impacted the kinetic parameters.•Organic solvent caused conformation chan...