Intrarenal Expression of miRNAs in Patients With Hypertensive Nephrosclerosis

• Published in: American journal of hypertension Vol. 23; no. 1; pp. 78 - 84
• Main Authors: Wang, Gang, Kwan, Bonnie Ching-Ha, Lai, Fernand Mac-Moune, Choi, Paul Cheung-Lung, Chow, Kai-Ming, Li, Philip Kam-Tao, Szeto, Cheuk-Chun
• Format: Journal Article
• Language: English
• Published: Basingstoke Oxford University Press 01.01.2010; Nature Publishing Group
• Subjects: Adult; Aged; Antihypertensive agents; Arterial hypertension. Arterial hypotension; Biological and medical sciences; Blood and lymphatic vessels; Blood Pressure; Cardiology. Vascular system; Cardiovascular system; Female; Glomerular Filtration Rate; Homeodomain Proteins - metabolism; Humans; hypertension; Hypertension - complications; Hypertension - physiopathology; Kidney - metabolism; Male; Medical sciences; microRNA; MicroRNAs - metabolism; Middle Aged; Nephrosclerosis - etiology; Nephrosclerosis - genetics; Nephrosclerosis - physiopathology; Pharmacology. Drug treatments; renal disease; Repressor Proteins - metabolism; Severity of Illness Index; Transcription Factors - metabolism; Up-Regulation; Zinc Finger E-box Binding Homeobox 2; Zinc Finger E-box-Binding Homeobox 1; blood pressure; microRNA; hypertension; renal disease; Human; Hypertension; Kidney disease; Urinary system disease; Cardiovascular disease; Arterial pressure; Blood pressure; Intrarenal
• DOI: 10.1038/ajh.2009.208
• ISSN: 0895-7061; 1941-7225

Background MicroRNAs (miRNAs) are non-coding, single-stranded RNA molecules that play important roles in a number of physiological and pathological processes. Previous studies showed that miRNAs targeting transcription factors ZEB1 and ZEB2 may repress epithelial–mesenchymal transition (EMT). Methods We studied 34 consecutive patients with biopsy-proven hypertensive nephrosclerosis. Intrarenal expression of miR-200 family, miR-205, and miR-192 were determined. We also studied normal renal tissue from 20 patients with nephrectomy for kidney cancer as controls. Results The level of intrarenal of miR-200a, miR-200b, miR-141, miR-429, miR-205, and miR-192 were significantly higher in patients with hypertensive nephrosclerosis than controls. Proteinuria correlated with intrarenal expression of miR-200a (r = 0.594, P < 0.001), miR-200b (r = 0.395, P = 0.004), miR-141 (r = 0.377, P = 0.007), miR-429 (r = 0.346, P = 0.013), miR-205 (r = 0.636, P < 0.001), and miR-192 (r = 0.306, P = 0.029). Estimated glomerular filtration rate (GFR) correlated with intrarenal expression of miR-200a (r = −0.374, P = 0.007) and miR-205 (r = −0.400, P = 0.005). Intrarenal expression of ZEB1 inversely correlated with intrarenal expression of miR-429, whereas expression of ZEB2 inversely correlated with miR-200a, miR-200b, and miR-429. Conclusions The results show that intrarenal expression of miR-200a, miR-200b, miR-141, miR-429, miR-205, and miR-192 were increased in hypertensive nephrosclerosis, and the degree of upregulation correlated with disease severity. The results suggested that these miRNA species may play important roles in the pathogenesis of hypertensive nephrosclerosis.