Organization and expression of the chum salmon insulin-like growth factor II gene

• Published in: FEBS letters Vol. 416; no. 3; pp. 344 - 348
• Main Authors: Palamarchuk, Alexey Y., Holthuizen, P.Elly, Müller, Werner E.G., Sussenbach, John S., Kavsan, Vadim M.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 27.10.1997
• Subjects: amino acid; Animals; base pair; Base Sequence; chum salmon IGF-II; coupled reverse transcription-polymerase chain reaction; DNA Primers; Exons; Fish; Freshwater; Gene expression; Gene Expression Regulation; Gene structure; Humans; IGF-II; Insulin-like growth factor II; Insulin-Like Growth Factor II - biosynthesis; Insulin-Like Growth Factor II - genetics; kbp; kilobase pair; Marine; Molecular Sequence Data; nucleotide; Oncorhynchus keta; Oncorhynchus keta - genetics; Oncorhynchus mykiss; Polymerase Chain Reaction; Promoter Regions, Genetic; Protein Precursors - biosynthesis; Protein Precursors - genetics; Recombinant Proteins - biosynthesis; RT-PCR; Salmon; SDS; sIGF-II; sodium dodecyl sulfate; SSC; standard sodium chloride/sodium citrate buffer; Transfection; Tumor Cells, Cultured; untranslated region; UTR; aa; SSC; SDS; Salmon; nt; Gene structure; Oncorhynchus keta; Gene expression; bp; UTR; RT-PCR; kbp; Fish; Insulin-like growth factor II; IGF-II; sIGF-II

IGF-II plays an important role in growth and development of vertebrates. In the present study, the characterization of the first fish IGF-II gene, chum salmon IGF-II, is described. The sIGF-II gene consists of four exons, spanning a region of 9 kbp, that encode the 214 aa IGF-II precursor. While the...