CCR1 chemokine receptor expression isolates erythroid from granulocyte-macrophage progenitors

• Published in: Journal of leukocyte biology Vol. 70; no. 3; pp. 455 - 460
• Main Authors: de Wynter, Erika A., Heyworth, Clare M., Mukaida, Naofumi, Jaworska, Ewa, Weffort‐Santos, Almeriane, Matushima, Kouji, Testa, Nydia G.
• Format: Journal Article
• Language: English
• Published: United States Society for Leukocyte Biology 01.09.2001
• Subjects: Antibodies - immunology; Antigens, CD34 - analysis; Biomarkers - analysis; CCR1 antibody; CCR1 protein; CD34 antigen; CD34+ cells; Cell Culture Techniques - methods; Cell Differentiation; Cell Lineage; Cells, Cultured; Chemokine CCL4; Colony-Forming Units Assay; Culture Media, Serum-Free; Erythroid Precursor Cells - chemistry; Erythroid Precursor Cells - cytology; Fetal Blood - cytology; Flow Cytometry; Granulocytes - cytology; Granulocytes - immunology; Humans; Macrophage Inflammatory Proteins - pharmacology; Macrophages - cytology; Macrophages - immunology; MIP‐1α; Myeloid Progenitor Cells - chemistry; Myeloid Progenitor Cells - cytology; Myeloid Progenitor Cells - immunology; myeloid progenitors; Receptors, CCR1; Receptors, Chemokine - biosynthesis; Receptors, Chemokine - genetics; Receptors, Chemokine - immunology; RNA, Messenger - biosynthesis

Simple methods that separate progenitor cells of different hemopoietic lineages would facilitate studies on lineage commitment and differentiation. We used an antibody specific for the chemokine receptor CCR1 to examine mononuclear cells isolated from cord blood samples. When CD34+ cells were separa...