CCR1 chemokine receptor expression isolates erythroid from granulocyte-macrophage progenitors
Simple methods that separate progenitor cells of different hemopoietic lineages would facilitate studies on lineage commitment and differentiation. We used an antibody specific for the chemokine receptor CCR1 to examine mononuclear cells isolated from cord blood samples. When CD34+ cells were separa...
Saved in:
Published in | Journal of leukocyte biology Vol. 70; no. 3; pp. 455 - 460 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Society for Leukocyte Biology
01.09.2001
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Simple methods that separate progenitor cells of different hemopoietic lineages would facilitate studies on lineage commitment and differentiation. We used an antibody specific for the chemokine receptor CCR1 to examine mononuclear cells isolated from cord blood samples. When CD34+ cells were separated into CD34+CCR1+ and CD34+CCR1− cells and plated in colony‐forming assays, the granulocyte/macrophage progenitors were found almost exclusively in the CD34+CCR1+ cells. In contrast, the CD34+CCR1− cells contained the majority of the erythroid progenitors. There was a highly significant difference (P<0.002) in the total percentage distribution of both granulocyte‐macrophage colony‐forming cells and erythroid burst‐forming units between the two populations. This is the first report of separation of erythroid progenitors from granulocyte/macrophage progenitors using a chemokine receptor antibody in cord blood samples. These results suggest that at the clonogenic progenitor cell stage the expression of CCR1 might be lineage‐specific. This method should prove useful for studies on erythroid progenitor and granulocyte/macrophage differentiation. |
---|---|
AbstractList | Simple methods that separate progenitor cells of different hemopoietic lineages would facilitate studies on lineage commitment and differentiation. We used an antibody specific for the chemokine receptor CCR1 to examine mononuclear cells isolated from cord blood samples. When CD34(+) cells were separated into CD34(+)CCR1(+) and CD34(+)CCR1(-) cells and plated in colony-forming assays, the granulocyte/macrophage progenitors were found almost exclusively in the CD34(+)CCR1(+) cells. In contrast, the CD34(+)CCR1(-) cells contained the majority of the erythroid progenitors. There was a highly significant difference (P<0.002) in the total percentage distribution of both granulocyte-macrophage colony-forming cells and erythroid burst-forming units between the two populations. This is the first report of separation of erythroid progenitors from granulocyte/macrophage progenitors using a chemokine receptor antibody in cord blood samples. These results suggest that at the clonogenic progenitor cell stage the expression of CCR1 might be lineage-specific. This method should prove useful for studies on erythroid progenitor and granulocyte/macrophage differentiation. Simple methods that separate progenitor cells of different hemopoietic lineages would facilitate studies on lineage commitment and differentiation. We used an antibody specific for the chemokine receptor CCR1 to examine mononuclear cells isolated from cord blood samples. When CD34+ cells were separated into CD34+CCR1+ and CD34+CCR1− cells and plated in colony‐forming assays, the granulocyte/macrophage progenitors were found almost exclusively in the CD34+CCR1+ cells. In contrast, the CD34+CCR1− cells contained the majority of the erythroid progenitors. There was a highly significant difference (P<0.002) in the total percentage distribution of both granulocyte‐macrophage colony‐forming cells and erythroid burst‐forming units between the two populations. This is the first report of separation of erythroid progenitors from granulocyte/macrophage progenitors using a chemokine receptor antibody in cord blood samples. These results suggest that at the clonogenic progenitor cell stage the expression of CCR1 might be lineage‐specific. This method should prove useful for studies on erythroid progenitor and granulocyte/macrophage differentiation. Simple methods that separate progenitor cells of different hemopoietic lineages would facilitate studies on lineage commitment and differentiation. We used an antibody specific for the chemokine receptor CCR1 to examine mononuclear cells isolated from cord blood samples. When CD34 super(+) cells were separated into CD34 super(+)CCR1 super(+) and CD34 super(+)CCR1 super(-) cells and plated in colony-forming assays, the granulocyte/macrophage progenitors were found almost exclusively in the CD34 super(+)CCR1 super(+) cells. In contrast, the CD34 super(+)CCR1 super(-) cells contained the majority of the erythroid progenitors. There was a highly significant difference (P<0.002) in the total percentage distribution of both granulocyte-macrophage colony-forming cells and erythroid burst-forming units between the two populations. This is the first report of separation of erythroid progenitors from granulocyte/macrophage progenitors using a chemokine receptor antibody in cord blood samples. These results suggest that at the clonogenic progenitor cell stage the expression of CCR1 might be lineage-specific. This method should prove useful for studies on erythroid progenitor and granulocyte/macrophage differentiation. Simple methods that separate progenitor cells of different hemopoietic lineages would facilitate studies on lineage commitment and differentiation. We used an antibody specific for the chemokine receptor CCR1 to examine mononuclear cells isolated from cord blood samples. When CD34(+) cells were separated into CD34(+)CCR1(+) and CD34(+)CCR1(-) cells and plated in colony-forming assays, the granulocyte/macrophage progenitors were found almost exclusively in the CD34(+)CCR1(+) cells. In contrast, the CD34(+)CCR1(-) cells contained the majority of the erythroid progenitors. There was a highly significant difference (P<0.002) in the total percentage distribution of both granulocyte-macrophage colony-forming cells and erythroid burst-forming units between the two populations. This is the first report of separation of erythroid progenitors from granulocyte/macrophage progenitors using a chemokine receptor antibody in cord blood samples. These results suggest that at the clonogenic progenitor cell stage the expression of CCR1 might be lineage-specific. This method should prove useful for studies on erythroid progenitor and granulocyte/macrophage differentiation. |
Author | Almeriane Weffort-Santos Nydia G. Testa Kouji Matushima Clare M. Heyworth Erika A. de Wynter Naofumi Mukaida Ewa Jaworska |
Author_xml | – sequence: 1 givenname: Erika A. surname: de Wynter fullname: de Wynter, Erika A. email: medeadw@leeds.ac.uk – sequence: 2 givenname: Clare M. surname: Heyworth fullname: Heyworth, Clare M. – sequence: 3 givenname: Naofumi surname: Mukaida fullname: Mukaida, Naofumi – sequence: 4 givenname: Ewa surname: Jaworska fullname: Jaworska, Ewa – sequence: 5 givenname: Almeriane surname: Weffort‐Santos fullname: Weffort‐Santos, Almeriane – sequence: 6 givenname: Kouji surname: Matushima fullname: Matushima, Kouji – sequence: 7 givenname: Nydia G. surname: Testa fullname: Testa, Nydia G. |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/11527996$$D View this record in MEDLINE/PubMed |
BookMark | eNqFkDuP1DAUhS20iJ1d6KhRGqjIcP2KkxJGPDUSEoISWY5zZ-LFiYOdUZh_j1cZQQfVbb7zHd1zQ67GMCIhTylsKa2bV3e-3SrY8q2Q8gHZ0IbXJa8UvyIbUIKWUgBck5uU7gCAswoekWtKJVNNU23I993uCy1sj0P44UYsIlqc5hAL_DVFTMmFsXApeDNjKjCe5z4G1xWHGIbiGM148sGeZywHY2OYenPEYorhiKPLkvSYPDwYn_DJ5d6Sb-_eft19KPef33_cvd6XVshKlmjbhgI1jPFGWM6bTlRKgbFCNJYJ1VkBworKNpKClC3vrG2RGYttV6scuCUvVm_u_nnCNOvBJYvemxHDKWlFKWdQs_-CtKYADO6NL1cwv5VSxIOeohtMPGsK-n53nXfXCjTXefeMP7t4T-2A3V_4MnQG6AoszuP5nzL9af8GVunzNdO7Y7-4iDoNxvtcwfSyLH_KfwNWap4C |
CitedBy_id | crossref_primary_10_1034_j_1600_065X_2002_18705_x crossref_primary_10_1186_s13075_018_1613_0 crossref_primary_10_1016_j_hlc_2022_11_017 crossref_primary_10_1038_sj_gt_3302422 crossref_primary_10_1146_annurev_immunol_21_120601_141007 crossref_primary_10_1016_j_devbrainres_2004_06_015 crossref_primary_10_1073_pnas_172384399 crossref_primary_10_1309_AJCP1TA3FLOQTMHF crossref_primary_10_1016_S0002_9440_10_63921_1 crossref_primary_10_1016_j_exphem_2004_11_015 crossref_primary_10_1046_j_1365_2141_2003_04204_x crossref_primary_10_1016_j_exphem_2005_06_028 crossref_primary_10_1152_ajpgi_00420_2006 |
Cites_doi | 10.1074/jbc.272.51.32078 10.1182/blood.V89.8.2757 10.1002/jlb.60.5.658 10.1182/blood.V81.12.3252.3252 10.1002/stem.160349 10.1182/blood.V79.10.2605.bloodjournal79102605 10.4049/jimmunol.149.3.1004 10.1182/blood.V92.10.3658 10.1634/stemcells.18-2-128 10.1074/jbc.270.33.19495 10.1182/blood.V93.10.3355.410k30_3355_3368 10.1182/blood.V90.2.605 10.1182/blood.V88.5.1594.1594 10.1182/blood.V76.6.1110.1110 10.1182/blood.V79.9.2221.2221 10.1016/0092-8674(93)90118-A 10.1084/jem.189.12.1987 10.1172/JCI112087 10.1182/blood.V79.3.642.642 10.1046/j.1365-2141.1999.01639.x 10.1099/0022-1317-79-1-71 10.1182/blood.V47.5.777.777 10.1084/jem.177.5.1421 10.1006/bcmd.1998.0167 10.1182/blood.V80.8.1940.1940 10.1084/jem.175.6.1501 10.1038/344442a0 10.1002/jcp.1041420202 10.1182/blood.V84.7.2175.2175 10.1182/blood.V86.11.4076.bloodjournal86114076 10.1182/blood.V86.12.4400.bloodjournal86124400 10.1084/jem.170.5.1583 10.1182/blood.V85.12.3412.bloodjournal85123412 10.1182/blood.V75.2.370.370 10.1182/blood.V82.9.2664.bloodjournal8292664 10.4049/jimmunol.150.8.3448 |
ContentType | Journal Article |
Copyright | 2001 Society for Leukocyte Biology |
Copyright_xml | – notice: 2001 Society for Leukocyte Biology |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7T5 H94 7X8 |
DOI | 10.1189/jlb.70.3.455 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Immunology Abstracts AIDS and Cancer Research Abstracts MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef AIDS and Cancer Research Abstracts Immunology Abstracts MEDLINE - Academic |
DatabaseTitleList | MEDLINE CrossRef AIDS and Cancer Research Abstracts MEDLINE - Academic |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Anatomy & Physiology Biology |
EISSN | 1938-3673 |
EndPage | 460 |
ExternalDocumentID | 10_1189_jlb_70_3_455 11527996 JLB0455 www70_3_455 |
Genre | article Research Support, Non-U.S. Gov't Journal Article |
GrantInformation_xml | – fundername: Cancer Research Campaign |
GroupedDBID | - 0VX 1OC 2WC 4.4 53G 5GY 5RE AAZKR ABCUV ABFLS ACGFS ACPOU ACPRK ACXQS ADACO ADBBV ADDAD ADXAS ADZMN AENEX AEUQT AFFNX AFRAH ALMA_UNASSIGNED_HOLDINGS ALUQN BFHJK BQCPF CS3 DCZOG DRFUL DRSTM DU5 E3Z EBS EJD F5P GJ GX1 H13 HZ K-O L7B LATKE LEEKS LUTES LYRES MEWTI MSJOP MXJOP OK1 P2P P2W RHF RHI ROL SJN SUPJJ TSL VH1 WOHZO WOQ ZA5 ZGI ZXP ZZTAW --- .GJ 0R~ 18M 1OB 29K 33P 5WD AABZA AACZT AAHHS AAPGJ AAPXW AARHZ AASGY AAUAY AAVAP AAWDT AAXRX ABEFU ABJNI ABLJU ABMNT ABNHQ ABPQP ABPTD ABWST ABXVV ACAHQ ACCFJ ACCZN ACFRR ACGFO ACUTJ ACXBN ACZBC ADIPN ADKYN ADOZA ADQBN ADVEK AEEZP AEQDE AFGWE AFYAG AGMDO AGQXC AI. AIURR AIWBW AJAOE AJBDE AJEEA AMYDB ANFBD APJGH ATGXG AVNTJ BCRHZ C45 D-I EMOBN F9R HZ~ KOP O9- OBOKY OCZFY OJZSN OPAEJ OVD OWPYF RJQFR ROX TCN TEORI TMA TR2 W8F YHG CGR CUY CVF ECM EIF NPM AAYXX ABEJV AHMMS CITATION 7T5 H94 7X8 |
ID | FETCH-LOGICAL-c4565-ecb9101a22394c339d46770ac449c247dc404c46c951055b3dccbe2acebd87c33 |
ISSN | 0741-5400 |
IngestDate | Tue Dec 03 23:09:32 EST 2024 Wed Dec 04 07:31:06 EST 2024 Fri Dec 06 03:02:26 EST 2024 Sat Sep 28 07:36:27 EDT 2024 Sat Aug 24 00:46:00 EDT 2024 Tue Jan 05 20:22:08 EST 2021 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 3 |
Language | English |
License | https://academic.oup.com/pages/standard-publication-reuse-rights |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c4565-ecb9101a22394c339d46770ac449c247dc404c46c951055b3dccbe2acebd87c33 |
Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
PMID | 11527996 |
PQID | 18100203 |
PQPubID | 23462 |
PageCount | 6 |
ParticipantIDs | proquest_miscellaneous_71132082 proquest_miscellaneous_18100203 crossref_primary_10_1189_jlb_70_3_455 pubmed_primary_11527996 wiley_primary_10_1189_jlb_70_3_455_JLB0455 highwire_smallpub2_www70_3_455 |
ProviderPackageCode | RHF RHI |
PublicationCentury | 2000 |
PublicationDate | September 2001 |
PublicationDateYYYYMMDD | 2001-09-01 |
PublicationDate_xml | – month: 09 year: 2001 text: September 2001 |
PublicationDecade | 2000 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | Journal of leukocyte biology |
PublicationTitleAlternate | J Leukoc Biol |
PublicationYear | 2001 |
Publisher | Society for Leukocyte Biology |
Publisher_xml | – name: Society for Leukocyte Biology |
References | 1990; 75 1992; 80 1990; 76 1976; 47 1993; 82 1997; 272 1993; 81 1997; 89 1992; 149 1992; 79 1993 1999; 189 1999; 106 1990; 344 1990; 142 1994; 84 1995; 270 1998; 24 1998; 16 1995; 86 1995; 85 1987; 69 1997; 90 2000; 18 1992; 175 1993; 72 1989; 170 1993; 150 1996; 60 1998; 92 1999; 93 1985; 76 1998; 36 1996; 88 1993; 177 1998; 79 Broxmeyer (2023033019261986000_) 1989; 170 Broxmeyer (2023033019261986000_) 1998; 24 Ratajczak (2023033019261986000_) 1998; 36 de Jong (2023033019261986000_) 1995; 86 Broxmeyer (2023033019261986000_) 1990; 76 Dunlop (2023033019261986000_) 1992; 79 Loken (2023033019261986000_) 1987; 69 Power (2023033019261986000_) 1995; 270 Ziegler (2023033019261986000_) 1999; 93 Hunter (2023033019261986000_) 1995; 86 Mayani (2023033019261986000_) 1993; 82 Mayani (2023033019261986000_) 1993; 81 Heath (2023033019261986000_) 1976; 47 Olweus (2023033019261986000_) 1996; 88 Kannourakis (2023033019261986000_) 1990; 75 Broxmeyer (2023033019261986000_) 1999; 189 Broxmeyer (2023033019261986000_) 1993; 150 Su (2023033019261986000_) 1997; 90 Sawada (2023033019261986000_) 1990; 142 Lansdorp (2023033019261986000_) 1992; 175 Maze (2023033019261986000_) 1992; 149 Carr (2023033019261986000_) 1998; 79 de Wynter (2023033019261986000_) 1998; 16 Emerson (2023033019261986000_) 1985; 76 Inada (2023033019261986000_) 1997; 89 Gao (2023033019261986000_) 1993; 177 Wickrema (2023033019261986000_) 1992; 80 Majka (2023033019261986000_) 2000; 18 Su (2023033019261986000_) 1996; 60 Lord (2023033019261986000_) 1995; 85 Freyssinier (2023033019261986000_) 1999; 106 Graham (2023033019261986000_) 1990; 344 Coutinho (2023033019261986000_) 1993 Panzenbock (2023033019261986000_) 1998; 92 Keller (2023033019261986000_) 1994; 84 Nibbs (2023033019261986000_) 1997; 272 Lord (2023033019261986000_) 1992; 79 Wognum (2023033019261986000_) 1992; 79 Neote (2023033019261986000_) 1993; 72 |
References_xml | – volume: 270 start-page: 19495 year: 1995 end-page: 19500 article-title: Molecular cloning and functional expression of a novel CC chemokine receptor cDNA from a human basophilic cell line publication-title: J. Biol. Chem. – volume: 85 start-page: 3412 year: 1995 end-page: 3415 article-title: Mobilization of early hematopoietic progenitor cells with BB‐10010: a genetically engineered variant of human macrophage inflammatory protein‐1 alpha publication-title: Blood – volume: 149 start-page: 1004 year: 1992 end-page: 1009 article-title: Myelosuppressive effects in vivo of purified recombinant murine macrophage inflammatory protein‐1 alpha publication-title: J. Immunol. – volume: 76 start-page: 1286 year: 1985 end-page: 1290 article-title: Purification of fetal hematopoietic progenitors and demonstration of recombinant multipotential colony‐stimulating activity publication-title: J. Clin. Invest. – start-page: 75 year: 1993 end-page: 106 – volume: 344 start-page: 442 year: 1990 end-page: 444 article-title: Identification and characterization of an inhibitor of haemopoietic stem cell proliferation publication-title: Nature – volume: 86 start-page: 4076 year: 1995 end-page: 4085 article-title: Separation of myeloid and erythroid progenitors based on expression of CD34 and c‐kit publication-title: Blood – volume: 106 start-page: 912 year: 1999 end-page: 922 article-title: Purification, amplification and characterization of a population of human erythroid progenitors publication-title: Br. J. Haematol. – volume: 150 start-page: 3448 year: 1993 end-page: 3458 article-title: Comparative analysis of the human macrophage inflammatory protein family of cytokines (chemokines) on proliferation of human myeloid progenitor cells publication-title: J. Immunol. – volume: 36 start-page: 55 year: 1998 end-page: 60 article-title: An improved serum free system for cloning human “pure” erythroid colonies: the role of different growth factors and cytokines on BFU‐E formation by the bone marrow and cord blood CD34+ cells publication-title: Fol. Histochem. Cytobiol. – volume: 75 start-page: 370 year: 1990 end-page: 377 article-title: Proliferative properties of unfractionated, purified, and single cell human progenitor populations stimulated by recombinant human interleukin‐3 publication-title: Blood – volume: 272 start-page: 32078 year: 1997 end-page: 32083 article-title: Cloning and characterization of a novel promiscuous human b‐chemokine receptor D6 publication-title: J. Biol. Chem. – volume: 79 start-page: 2605 year: 1992 end-page: 2609 article-title: Macrophage‐inflammatory protein protects multipotent hematopoietic cells from the cytotoxic effects of hydroxyurea in vivo publication-title: Blood – volume: 189 start-page: 1987 year: 1999 end-page: 1992 article-title: Dominant myelopoietic effector functions mediated by chemokine receptor CCR1 publication-title: J. Exp. Med. – volume: 16 start-page: 349 year: 1998 end-page: 356 article-title: Differential response of CD34+ cells isolated from cord blood and bone marrow to MIP‐1 alpha and the expression of MIP‐1 alpha receptors on these immature cells publication-title: Stem Cells – volume: 170 start-page: 1583 year: 1989 end-page: 1594 article-title: Myelopoietic enhancing effects of murine macrophage inflammatory proteins 1 and 2 on colony formation in vitro by murine and human bone marrow granulocyte/macrophage progenitor cells publication-title: J. Exp. Med. – volume: 72 start-page: 415 year: 1993 end-page: 425 article-title: Molecular cloning, functional expression, and signaling characteristics of a C‐C chemokine receptor publication-title: Cell – volume: 93 start-page: 3355 year: 1999 end-page: 3368 article-title: Unicellular‐unilineage erythropoietic cultures: molecular analysis of regulatory gene expression at sibling cell level publication-title: Blood – volume: 84 start-page: 2175 year: 1994 end-page: 2181 article-title: Distinct and overlapping direct effects of macrophage inflammatory protein‐1 α and transforming growth factor β on hematopoietic progenitor/stem cell growth publication-title: Blood – volume: 89 start-page: 2757 year: 1997 end-page: 2765 article-title: Selective expression of the receptor tyrosine kinase, HTK, on human erythroid progenitor cells publication-title: Blood – volume: 18 start-page: 128 year: 2000 end-page: 138 article-title: The role of HIV‐related chemokine receptors and chemokines in human erythropoiesis in vitro publication-title: Stem Cells – volume: 90 start-page: 605 year: 1997 end-page: 611 article-title: Inhibition of immature erythroid progenitor cell proliferation by macrophage inflammatory protein‐1alpha by interacting mainly with a C‐C chemokine receptor, CCR1 publication-title: Blood – volume: 142 start-page: 219 year: 1990 end-page: 230 article-title: Purification of human blood burst‐forming units‐erythroid and demonstration of the evolution of erythropoietin receptors publication-title: J. Cell Physiol. – volume: 79 start-page: 642 year: 1992 end-page: 649 article-title: Increased erythropoietin‐receptor expression on CD34‐positive bone marrow cells from patients with chronic myeloid leukemia publication-title: Blood – volume: 88 start-page: 1594 year: 1996 end-page: 1607 article-title: Expression and function of receptors for stem cell factor and erythropoietin during lineage commitment of human hematopoietic progenitor cells publication-title: Blood – volume: 92 start-page: 3658 year: 1998 end-page: 3668 article-title: Growth and differentiation of human stem cell factor/erythropoietin‐dependent erythroid progenitor cells in vitro publication-title: Blood – volume: 79 start-page: 2221 year: 1992 end-page: 2225 article-title: Demonstration of stem cell inhibition and myeloprotective effects of SCI/rhMIP1 alpha in vivo publication-title: Blood – volume: 79 start-page: 71 year: 1998 end-page: 75 article-title: CD34+ cells and their derivatives contain mRNA for CD4 and human immunodeficiency virus (HIV) co‐receptors and are susceptible to infection with M‐ and T‐tropic HIV publication-title: J. Gen. Virol. – volume: 175 start-page: 1501 year: 1992 end-page: 1509 article-title: Long‐term erythropoiesis from constant numbers of CD34+ cells in serum‐free cultures initiated with highly purified progenitor cells from human bone marrow publication-title: J. Exp. Med. – volume: 24 start-page: 14 year: 1998 end-page: 30 article-title: Myeloid progenitor cell proliferation and mobilization effects of BB10010, a genetically engineered variant of human macrophage inflammatory protein‐1alpha, in a phase I clinical trial in patients with relapsed/refractory breast cancer publication-title: Blood Cells Mol. Dis. – volume: 86 start-page: 4400 year: 1995 end-page: 4408 article-title: BB‐10010: an active variant of human macrophage inflammatory protein‐1 alpha with improved pharmaceutical properties publication-title: Blood – volume: 69 start-page: 255 year: 1987 end-page: 263 article-title: Flow cytometric analysis of human bone marrow publication-title: I. Normal erythroid development. Blood – volume: 47 start-page: 777 year: 1976 end-page: 792 article-title: Separation of the erythropoietin‐responsive progenitors BFU‐E and CFU‐E in mouse bone marrow by unit gravity sedimentation publication-title: Blood – volume: 82 start-page: 2664 year: 1993 end-page: 2672 article-title: Characterization of functionally distinct subpopulations of CD34+ cord blood cells in serum‐free long‐term cultures supplemented with hematopoietic cytokines publication-title: Blood – volume: 76 start-page: 1110 year: 1990 end-page: 1116 article-title: Enhancing and suppressing effects of recombinant murine macrophage inflammatory proteins on colony formation in vitro by bone marrow myeloid progenitor cells publication-title: Blood – volume: 80 start-page: 1940 year: 1992 end-page: 1949 article-title: Differentiation and erythropoietin receptor gene expression in human erythroid progenitor cells publication-title: Blood – volume: 177 start-page: 1421 year: 1993 end-page: 1427 article-title: Structure and functional expression of the human macrophage inflammatory protein 1 alpha/RANTES receptor publication-title: J. Exp. Med. – volume: 60 start-page: 658 year: 1996 end-page: 666 article-title: Preparation of specific polyclonal antibodies to a C‐C chemokine receptor, CCR1, and determination of CCR1 expression on various types of leukocytes publication-title: J. Leukoc. Biol. – volume: 81 start-page: 3252 year: 1993 end-page: 3258 article-title: Cytokine‐induced selective expansion and maturation of erythroid versus myeloid progenitors from purified cord blood precursor cells publication-title: Blood – volume: 272 start-page: 32078 year: 1997 ident: 2023033019261986000_ article-title: Cloning and characterization of a novel promiscuous human b-chemokine receptor D6 publication-title: J. Biol. Chem. doi: 10.1074/jbc.272.51.32078 contributor: fullname: Nibbs – volume: 89 start-page: 2757 year: 1997 ident: 2023033019261986000_ article-title: Selective expression of the receptor tyrosine kinase, HTK, on human erythroid progenitor cells publication-title: Blood doi: 10.1182/blood.V89.8.2757 contributor: fullname: Inada – volume: 60 start-page: 658 year: 1996 ident: 2023033019261986000_ article-title: Preparation of specific polyclonal antibodies to a C-C chemokine receptor, CCR1, and determination of CCR1 expression on various types of leukocytes publication-title: J. Leukoc. Biol. doi: 10.1002/jlb.60.5.658 contributor: fullname: Su – volume: 81 start-page: 3252 year: 1993 ident: 2023033019261986000_ article-title: Cytokine-induced selective expansion and maturation of erythroid versus myeloid progenitors from purified cord blood precursor cells publication-title: Blood doi: 10.1182/blood.V81.12.3252.3252 contributor: fullname: Mayani – volume: 16 start-page: 349 year: 1998 ident: 2023033019261986000_ article-title: Differential response of CD34+ cells isolated from cord blood and bone marrow to MIP-1 alpha and the expression of MIP-1 alpha receptors on these immature cells publication-title: Stem Cells doi: 10.1002/stem.160349 contributor: fullname: de Wynter – volume: 79 start-page: 2605 year: 1992 ident: 2023033019261986000_ article-title: Macrophage-inflammatory protein protects multipotent hematopoietic cells from the cytotoxic effects of hydroxyurea in vivo publication-title: Blood doi: 10.1182/blood.V79.10.2605.bloodjournal79102605 contributor: fullname: Lord – volume: 149 start-page: 1004 year: 1992 ident: 2023033019261986000_ article-title: Myelosuppressive effects in vivo of purified recombinant murine macrophage inflammatory protein-1 alpha publication-title: J. Immunol. doi: 10.4049/jimmunol.149.3.1004 contributor: fullname: Maze – volume: 92 start-page: 3658 year: 1998 ident: 2023033019261986000_ article-title: Growth and differentiation of human stem cell factor/erythropoietin-dependent erythroid progenitor cells in vitro publication-title: Blood doi: 10.1182/blood.V92.10.3658 contributor: fullname: Panzenbock – volume: 18 start-page: 128 year: 2000 ident: 2023033019261986000_ article-title: The role of HIV-related chemokine receptors and chemokines in human erythropoiesis in vitro publication-title: Stem Cells doi: 10.1634/stemcells.18-2-128 contributor: fullname: Majka – volume: 270 start-page: 19495 year: 1995 ident: 2023033019261986000_ article-title: Molecular cloning and functional expression of a novel CC chemokine receptor cDNA from a human basophilic cell line publication-title: J. Biol. Chem. doi: 10.1074/jbc.270.33.19495 contributor: fullname: Power – volume: 93 start-page: 3355 year: 1999 ident: 2023033019261986000_ article-title: Unicellular-unilineage erythropoietic cultures: molecular analysis of regulatory gene expression at sibling cell level publication-title: Blood doi: 10.1182/blood.V93.10.3355.410k30_3355_3368 contributor: fullname: Ziegler – volume: 90 start-page: 605 year: 1997 ident: 2023033019261986000_ article-title: Inhibition of immature erythroid progenitor cell proliferation by macrophage inflammatory protein-1alpha by interacting mainly with a C-C chemokine receptor, CCR1 publication-title: Blood doi: 10.1182/blood.V90.2.605 contributor: fullname: Su – volume: 88 start-page: 1594 year: 1996 ident: 2023033019261986000_ article-title: Expression and function of receptors for stem cell factor and erythropoietin during lineage commitment of human hematopoietic progenitor cells publication-title: Blood doi: 10.1182/blood.V88.5.1594.1594 contributor: fullname: Olweus – volume: 76 start-page: 1110 year: 1990 ident: 2023033019261986000_ article-title: Enhancing and suppressing effects of recombinant murine macrophage inflammatory proteins on colony formation in vitro by bone marrow myeloid progenitor cells publication-title: Blood doi: 10.1182/blood.V76.6.1110.1110 contributor: fullname: Broxmeyer – volume: 79 start-page: 2221 year: 1992 ident: 2023033019261986000_ article-title: Demonstration of stem cell inhibition and myeloprotective effects of SCI/rhMIP1 alpha in vivo publication-title: Blood doi: 10.1182/blood.V79.9.2221.2221 contributor: fullname: Dunlop – volume: 72 start-page: 415 year: 1993 ident: 2023033019261986000_ article-title: Molecular cloning, functional expression, and signaling characteristics of a C-C chemokine receptor publication-title: Cell doi: 10.1016/0092-8674(93)90118-A contributor: fullname: Neote – volume: 189 start-page: 1987 year: 1999 ident: 2023033019261986000_ article-title: Dominant myelopoietic effector functions mediated by chemokine receptor CCR1 publication-title: J. Exp. Med. doi: 10.1084/jem.189.12.1987 contributor: fullname: Broxmeyer – volume: 76 start-page: 1286 year: 1985 ident: 2023033019261986000_ article-title: Purification of fetal hematopoietic progenitors and demonstration of recombinant multipotential colony-stimulating activity publication-title: J. Clin. Invest. doi: 10.1172/JCI112087 contributor: fullname: Emerson – volume: 79 start-page: 642 year: 1992 ident: 2023033019261986000_ article-title: Increased erythropoietin-receptor expression on CD34-positive bone marrow cells from patients with chronic myeloid leukemia publication-title: Blood doi: 10.1182/blood.V79.3.642.642 contributor: fullname: Wognum – volume: 106 start-page: 912 year: 1999 ident: 2023033019261986000_ article-title: Purification, amplification and characterization of a population of human erythroid progenitors publication-title: Br. J. Haematol. doi: 10.1046/j.1365-2141.1999.01639.x contributor: fullname: Freyssinier – volume: 79 start-page: 71 year: 1998 ident: 2023033019261986000_ article-title: CD34+ cells and their derivatives contain mRNA for CD4 and human immunodeficiency virus (HIV) co-receptors and are susceptible to infection with M- and T-tropic HIV publication-title: J. Gen. Virol. doi: 10.1099/0022-1317-79-1-71 contributor: fullname: Carr – volume: 47 start-page: 777 year: 1976 ident: 2023033019261986000_ article-title: Separation of the erythropoietin-responsive progenitors BFU-E and CFU-E in mouse bone marrow by unit gravity sedimentation publication-title: Blood doi: 10.1182/blood.V47.5.777.777 contributor: fullname: Heath – volume: 177 start-page: 1421 year: 1993 ident: 2023033019261986000_ article-title: Structure and functional expression of the human macrophage inflammatory protein 1 alpha/RANTES receptor publication-title: J. Exp. Med. doi: 10.1084/jem.177.5.1421 contributor: fullname: Gao – volume: 24 start-page: 14 year: 1998 ident: 2023033019261986000_ article-title: Myeloid progenitor cell proliferation and mobilization effects of BB10010, a genetically engineered variant of human macrophage inflammatory protein-1alpha, in a phase I clinical trial in patients with relapsed/refractory breast cancer publication-title: Blood Cells Mol. Dis. doi: 10.1006/bcmd.1998.0167 contributor: fullname: Broxmeyer – start-page: 75 volume-title: Haemopoiesis: a Practical Approach year: 1993 ident: 2023033019261986000_ contributor: fullname: Coutinho – volume: 80 start-page: 1940 year: 1992 ident: 2023033019261986000_ article-title: Differentiation and erythropoietin receptor gene expression in human erythroid progenitor cells publication-title: Blood doi: 10.1182/blood.V80.8.1940.1940 contributor: fullname: Wickrema – volume: 175 start-page: 1501 year: 1992 ident: 2023033019261986000_ article-title: Long-term erythropoiesis from constant numbers of CD34+ cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow publication-title: J. Exp. Med. doi: 10.1084/jem.175.6.1501 contributor: fullname: Lansdorp – volume: 344 start-page: 442 year: 1990 ident: 2023033019261986000_ article-title: Identification and characterization of an inhibitor of haemopoietic stem cell proliferation publication-title: Nature doi: 10.1038/344442a0 contributor: fullname: Graham – volume: 142 start-page: 219 year: 1990 ident: 2023033019261986000_ article-title: Purification of human blood burst-forming units-erythroid and demonstration of the evolution of erythropoietin receptors publication-title: J. Cell Physiol. doi: 10.1002/jcp.1041420202 contributor: fullname: Sawada – volume: 84 start-page: 2175 year: 1994 ident: 2023033019261986000_ article-title: Distinct and overlapping direct effects of macrophage inflammatory protein-1 α and transforming growth factor β on hematopoietic progenitor/stem cell growth publication-title: Blood doi: 10.1182/blood.V84.7.2175.2175 contributor: fullname: Keller – volume: 86 start-page: 4076 year: 1995 ident: 2023033019261986000_ article-title: Separation of myeloid and erythroid progenitors based on expression of CD34 and c-kit publication-title: Blood doi: 10.1182/blood.V86.11.4076.bloodjournal86114076 contributor: fullname: de Jong – volume: 86 start-page: 4400 year: 1995 ident: 2023033019261986000_ article-title: BB-10010: an active variant of human macrophage inflammatory protein-1 alpha with improved pharmaceutical properties publication-title: Blood doi: 10.1182/blood.V86.12.4400.bloodjournal86124400 contributor: fullname: Hunter – volume: 170 start-page: 1583 year: 1989 ident: 2023033019261986000_ article-title: Myelopoietic enhancing effects of murine macrophage inflammatory proteins 1 and 2 on colony formation in vitro by murine and human bone marrow granulocyte/macrophage progenitor cells publication-title: J. Exp. Med. doi: 10.1084/jem.170.5.1583 contributor: fullname: Broxmeyer – volume: 85 start-page: 3412 year: 1995 ident: 2023033019261986000_ article-title: Mobilization of early hematopoietic progenitor cells with BB-10010: a genetically engineered variant of human macrophage inflammatory protein-1 alpha publication-title: Blood doi: 10.1182/blood.V85.12.3412.bloodjournal85123412 contributor: fullname: Lord – volume: 75 start-page: 370 year: 1990 ident: 2023033019261986000_ article-title: Proliferative properties of unfractionated, purified, and single cell human progenitor populations stimulated by recombinant human interleukin-3 publication-title: Blood doi: 10.1182/blood.V75.2.370.370 contributor: fullname: Kannourakis – volume: 36 start-page: 55 year: 1998 ident: 2023033019261986000_ article-title: An improved serum free system for cloning human “pure” erythroid colonies: the role of different growth factors and cytokines on BFU-E formation by the bone marrow and cord blood CD34+ cells publication-title: Fol. Histochem. Cytobiol. contributor: fullname: Ratajczak – volume: 82 start-page: 2664 year: 1993 ident: 2023033019261986000_ article-title: Characterization of functionally distinct subpopulations of CD34+ cord blood cells in serum-free long-term cultures supplemented with hematopoietic cytokines publication-title: Blood doi: 10.1182/blood.V82.9.2664.bloodjournal8292664 contributor: fullname: Mayani – volume: 150 start-page: 3448 year: 1993 ident: 2023033019261986000_ article-title: Comparative analysis of the human macrophage inflammatory protein family of cytokines (chemokines) on proliferation of human myeloid progenitor cells publication-title: J. Immunol. doi: 10.4049/jimmunol.150.8.3448 contributor: fullname: Broxmeyer – volume: 69 start-page: 255 year: 1987 ident: 2023033019261986000_ article-title: Flow cytometric analysis of human bone marrow publication-title: I. Normal erythroid development. Blood contributor: fullname: Loken |
SSID | ssj0003260 |
Score | 1.7905414 |
Snippet | Simple methods that separate progenitor cells of different hemopoietic lineages would facilitate studies on lineage commitment and differentiation. We used an... |
SourceID | proquest crossref pubmed wiley highwire |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 455 |
SubjectTerms | Antibodies - immunology Antigens, CD34 - analysis Biomarkers - analysis CCR1 antibody CCR1 protein CD34 antigen CD34+ cells Cell Culture Techniques - methods Cell Differentiation Cell Lineage Cells, Cultured Chemokine CCL4 Colony-Forming Units Assay Culture Media, Serum-Free Erythroid Precursor Cells - chemistry Erythroid Precursor Cells - cytology Fetal Blood - cytology Flow Cytometry Granulocytes - cytology Granulocytes - immunology Humans Macrophage Inflammatory Proteins - pharmacology Macrophages - cytology Macrophages - immunology MIP‐1α Myeloid Progenitor Cells - chemistry Myeloid Progenitor Cells - cytology Myeloid Progenitor Cells - immunology myeloid progenitors Receptors, CCR1 Receptors, Chemokine - biosynthesis Receptors, Chemokine - genetics Receptors, Chemokine - immunology RNA, Messenger - biosynthesis |
Title | CCR1 chemokine receptor expression isolates erythroid from granulocyte-macrophage progenitors |
URI | http://www.jleukbio.org/content/70/3/455.abstract https://onlinelibrary.wiley.com/doi/abs/10.1189%2Fjlb.70.3.455 https://www.ncbi.nlm.nih.gov/pubmed/11527996 https://search.proquest.com/docview/18100203 https://search.proquest.com/docview/71132082 |
Volume | 70 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bb9MwFLZgCImXCTYu5TL8ADxQpTiJ0ySPo-qoqm5IqBV9QZZju9C1Tac1UQm_nmPnWq1cX6IqslPrfMfHn-1zQeiV53TB6NnA3OyZZ1Huw5wjQlh-ICTxlCKhKQd0ftEdTOhw6k3rckcmuiSJOuLH3riS_0EV3gGuOkr2H5CtPgov4DfgC09AGJ5_hXGv98lug9RX64Umi2C81FWiU3h_L9xb4_YcRqDpZFtdZ7okwlzmESVfYY1KYSHLEmWtuK7j9U1772h3LaVneXHJc5O2LlW6MN3aRf6mEjKp2p-zuKjz0a_PSAcqq_wQG4ev5-mCzyXfuQ0a6gSumwWvIyTK8wi7crgqzRZwFO1uQZo2Ni8OUuiS2zCYNE_SW6y9NK8tcNOsBzor6uUy6vik43aqTs3s2Rcf2dlkNGLj_nR8G93RiRF1LYUP09rlB6gqydOy5iMsAyGC8F3z27sUpUwbvW8LsrujMZRkfB8dFqDg01wxHqBbKj5Cx6cxT9arDL_BxrvXYHSE7uZFR7Nj9EVrDa60Bpdag2utwaXW4EprsNYavF9rcENrHqLJWX_cG1hFkQ1LaDJvKREBY7Q50MSQCtcNJSydPuGC0lA41JeCEipoVxgq7kWuFCJSDhcqkoEPHR6hg3gdqycIk65yQ0586YWC-mQW0XAmRQAS5ZEthNdCr0u5sqs8lwoze9AgZCB_5hPmMmjdQiel0NlmxZdLELbDtttt3eBlCQUDa6ivuHis1umGAV81d-u_buHbOmlA4LTQ4xzDeii6wjNs_1vorQH1t2Nkw9F72A15T__4R8_QvXqaPEcHyXWqXgCRTaITo50_AaR_oV4 |
link.rule.ids | 314,780,784,27924,27925 |
linkProvider | Geneva Foundation for Medical Education and Research |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=CCR1+chemokine+receptor+expression+isolates+erythroid+from+granulocyte-macrophage+progenitors&rft.jtitle=Journal+of+leukocyte+biology&rft.au=de+Wynter%2C+E+A&rft.au=Heyworth%2C+C+M&rft.au=Mukaida%2C+N&rft.au=Jaworska%2C+E&rft.date=2001-09-01&rft.issn=0741-5400&rft.volume=70&rft.issue=3&rft.spage=455&rft.epage=460&rft_id=info:doi/10.1189%2Fjlb.70.3.455&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0741-5400&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0741-5400&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0741-5400&client=summon |