Improved squalene production through increasing lipid contents in Saccharomyces cerevisiae

• Published in: Biotechnology and bioengineering Vol. 115; no. 7; pp. 1793 - 1800
• Main Authors: Wei, Liu‐Jing, Kwak, Suryang, Liu, Jing‐Jing, Lane, Stephan, Hua, Qiang, Kweon, Dae‐Hyuk, Jin, Yong‐Su
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.07.2018
• Subjects: ABC transporter; Accumulation; Acyl-CoA Oxidase - genetics; Acyl-CoA Oxidase - metabolism; ATP-Binding Cassette Transporters - genetics; ATP-Binding Cassette Transporters - metabolism; Baking yeast; Biofuels; Biosynthesis; diacylglycerol acyltransferase; Diacylglycerol O-Acyltransferase - genetics; Diacylglycerol O-Acyltransferase - metabolism; Diglycerides; Fermentation; Flavors; Gene Expression; HMG‐CoA reductase; Hydrophobicity; Hydroxymethylglutaryl CoA Reductases - genetics; Hydroxymethylglutaryl CoA Reductases - metabolism; lipid bodies; Lipid Metabolism; Lipids; Metabolic Engineering - methods; Metabolic Networks and Pathways - genetics; Mevalonic acid; Microorganisms; Molecular chains; Mutant Proteins - genetics; Mutant Proteins - metabolism; Oxidation; Pharmacology; Plant extracts; Plant tissues; Reductase; Saccharomyces cerevisiae; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Squalene; Squalene - metabolism; Storage capacity; Yeast; β‐oxidation; β-oxidation; diacylglycerol acyltransferase; HMG-CoA reductase; Saccharomyces cerevisiae; squalene; lipid bodies

Squalene, a valuable acyclic triterpene, can be used as a chemical commodity for pharmacology, flavor, and biofuel industries. Microbial production of squalene has been of great interest due to its limited availability, and increasing prices extracted from animal and plant tissues. Here we report ge...