Translational enhancing activity in 5′ UTR of peste des petits ruminants virus fusion gene

• Published in: The FEBS journal Vol. 280; no. 5; pp. 1237 - 1248
• Main Authors: Chulakasian, Songkhla, Chang, Tien‐Jye, Tsai, Ching‐Hsiu, Wong, Min‐Liang, Hsu, Wei‐Li
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.03.2013
• Subjects: 5' Untranslated Regions - genetics; 5′ UTR; Animal diseases; Animals; Base Sequence; Bioassays; Blotting, Northern; Cells, Cultured; Dogs; Enhancer Elements, Genetic - genetics; Flow Cytometry; fusion gene; Gene expression; Genes, Reporter; Humans; Kidney - cytology; Kidney - metabolism; Molecular Sequence Data; mRNA stability; Mutation - genetics; Paramyxovirus; peste des petits ruminants virus; Peste-des-petits-ruminants virus - genetics; Protein Biosynthesis - genetics; Ribonucleic acid; RNA; RNA Stability; Ruminantia; Sequence Deletion; Sequence Homology, Nucleic Acid; Transcription, Genetic - genetics; translational enhancer; Viral Fusion Proteins - genetics

The fusion gene of peste des petits ruminants virus (PPRV‐F), a paramyxovirus, contains an unusual long 5′ untranslated region (5′ UTR) with a high GC content that is capable of folding into secondary structure proximally to the 5′ cap. Sequence analysis further suggested that the proximal end of th...