Identification of secretin diastereoisomers produced during synthesis

• Published in: Journal of pharmaceutical sciences Vol. 78; no. 2; p. 91
• Main Authors: Tsuda, T, Uchiyama, M, Sato, T, Yoshino, H, Tsuchiya, Y, Ishikawa, S, Ohmae, M, Watanabe, S, Miyake, Y
• Format: Journal Article
• Language: English
• Published: United States 01.02.1989
• Subjects: Amino Acids - analysis; Chromatography, Gas; Chromatography, High Pressure Liquid; Chymotrypsin; D-Amino-Acid Oxidase; Hydrolysis; Indicators and Reagents; Secretin - analysis; Secretin - chemical synthesis; Secretin - isolation & purification; Stereoisomerism; Trypsin

The byproducts P-1 and P-2, which were produced during the synthesis of porcine secretin, were isolated in pure form from the crude secretin by HPLC. These were identified by a combination of amino acid analysis, enzymatic digestion, and isocratic or linear gradient reversed-phase (RP)-HPLC. The ami...