Structures of Streptococcus pyogenes class A sortase in complex with substrate and product mimics provide key details of target recognition

• Published in: The Journal of biological chemistry Vol. 298; no. 10; p. 102446
• Main Authors: Johnson, D. Alex, Piper, Isabel M., Vogel, Brandon A., Jackson, Sophie N., Svendsen, Justin E., Kodama, Hanna M., Lee, Darren E., Lindblom, Katy M., McCarty, James, Antos, John M., Amacher, Jeanine F.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2022; American Society for Biochemistry and Molecular Biology
• Subjects: BASIC BIOLOGICAL SCIENCES; Biochemistry & Molecular Biology; Enzymes; INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Protein engineering; Sortases; Structural biology; Target selectivity; NSF; saSrtA; MS; RMSF; enzymes; MW; CWSS; MurNAc; sortases; LII; PDB; SEC; protein engineering; baSrtA; LPXTG; SML; spySrtA; MD; Abz; structural biology; ESI; target selectivity

The cell wall is a critical extracellular barrier for bacteria and many other organisms. In bacteria, this structural layer consists of peptidoglycan, which maintains cell shape and structural integrity and provides a scaffold for displaying various protein factors. To attach proteins to the cell wa...