A molecular imprinting-based turn-on Ratiometric fluorescence sensor for highly selective and sensitive detection of 2,4-dichlorophenoxyacetic acid (2,4-D)

• Published in: Biosensors & bioelectronics Vol. 81; pp. 438 - 444
• Main Authors: Wang, Xiaoyan, Yu, Jialuo, Wu, Xiaqing, Fu, Junqing, Kang, Qi, Shen, Dazhong, Li, Jinhua, Chen, Lingxin
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.07.2016
• Subjects: 2,4-D; 2,4-Dichlorophenoxyacetic acid (2,4-D); 2,4-Dichlorophenoxyacetic Acid - analysis; Biosensors; color; Constants; detection limit; Drinking Water - analysis; Electron transfer; Electron Transport; Enrichment; Fluorescence; Fluorescence turn-on; Herbicides - analysis; Lakes - analysis; Limit of Detection; Molecular imprinting; Molecular Imprinting - methods; Photoinduced electron transfer; Polymerization; Quantum dots; Quantum Dots - chemistry; Quantum Dots - ultrastructure; Ratiometric fluorescence; Sensors; Spectrometry, Fluorescence - methods; Water Pollutants, Chemical - analysis; Water sample; Ratiometric fluorescence; Water sample; 2,4-Dichlorophenoxyacetic acid (2,4-D); Molecular imprinting; Photoinduced electron transfer; Fluorescence turn-on
• DOI: 10.1016/j.bios.2016.03.031
• ISSN: 0956-5663; 1873-4235

A novel molecular imprinting-based turn-on ratiometric fluorescence sensor was constructed via a facile sol–gel polymerization for detection of 2,4-dichlorophenoxyacetic acid (2,4-D) on the basis of photoinduced electron transfer (PET) by using nitrobenzoxadiazole (NBD) as detection signal source and quantum dots (QDs) as reference signal source. With the presence and increase of 2,4-D, the amine groups on the surface of QDs@SiO2 could bind with 2,4-D and thereby the NBD fluorescence intensities could be significantly enhanced since the PET process was inhibited, while the QDs maintained constant intensities. Accordingly, the ratio of the dual-emission intensities of green NBD and red QDs could be utilized for turn-on fluorescent detection of 2,4-D, along with continuous color changes from orange-red to green readily observed by the naked eye. The as-prepared fluorescence sensor obtained high sensitivity with a low detection limit of 0.14μM within 5min, and distinguished recognition selectivity for 2,4-D over its analogs. Moreover, the sensor was successfully applied to determine 2,4-D in real water samples, and high recoveries at three spiking levels of 2,4-D ranged from 95.0% to 110.1% with precisions below 4.5%. The simple, rapid and reliable visual sensing strategy would not only provide potential applications for high selective ultratrace analysis of complicated matrices, but also greatly enrich the research connotations of molecularly imprinted sensors. •Selective MIPs and ratiometric fluorescence was combined for 2,4-D detection.•Sensing mechanism was photoinduced electron transfer (PET) fluorescence enhancing.•The obtained sensor proved highly sensitive, selective, reliable and practical.