Cellular CD4 T Cell Responses to the Diphtheria- Derived Carrier Protein of Conjugated Pneumococcal Vaccine and Antibody Response to Pneumococcal Vaccination in HIV-Infected Adults

• Published in: Clinical infectious diseases Vol. 50; no. 8; pp. 1174 - 1183
• Main Authors: Rabian, Claire, Tschöpe, Inga, Lesprit, Philippe, Katlama, Christine, Molina, Jean-Michel, Meynard, Jean-Luc, Delfraissy, Jean-François, Cheêne, Geneviève, Lévy, Yves
• Format: Journal Article
• Language: English
• Published: United States The University of Chicago Press 15.04.2010; University of Chicago Press; Oxford University Press
• Subjects: Adult; Antibodies; Antibodies, Bacterial - blood; B lymphocytes; Bacterial Proteins - immunology; Carrier Proteins - immunology; CD4-Positive T-Lymphocytes - immunology; Cell Proliferation; Conjugate vaccines; Cytokines; Diphtheria; Female; Heptavalent Pneumococcal Conjugate Vaccine; HIV; HIV Infections - immunology; HIV/AIDS; Human immunodeficiency virus; Humans; Immunoglobulin G - blood; Immunoglobulins; Immunologic Memory; Immunology; Male; Middle Aged; Pneumococcal vaccine; Pneumococcal Vaccines - immunology; Polysaccharides; Streptococcus infections; Streptococcus pneumoniae; T cell receptors; T lymphocytes; Th1 Cells - immunology; Vaccination; Vaccination - methods; Vaccines
• ISSN: 1058-4838; 1537-6591
• DOI: 10.1086/651418

Background. The French National Agency for AIDS and Viral Hepatitis Research (ANRS) 114 Pneumovac trial showed that a strategy combining a 7-valent pneumococcal conjugate vaccine (PCV) prime at week 0 followed by a 23-valent pneumococcal polysaccharide vaccine (PPV) boost at week 4 enhances the frequency and magnitude of immunoglobulin (Ig) G responses against Streptococcus pneumoniae polysaccharides (SPPs) compared with PPV alone. CD4 T cell responses specific to the diphtheria-derived carrier protein CRM197 were evaluated. Methods. Lymphocyte proliferative responses (LPRs) and TH1 cytokine T cell responses against the diphtheriaderived carrier protein CRM197 contained in the PCV were investigated at weeks 0, 4, and 24. Results. In the prime-boost PCV and PPV group, the magnitude of LPRs to diphtheria toxoid and CRM197 increased at week 4 (P < .001) and persisted until week 24 (P = .08 and .13, respectively, compared with week 4). Interferon-γ and interleukin-2 production to CRM197 increased significantly at week 4 (P = .02 and P < .001, respectively) and remained stable until week 24 (P = .28 and P = .08, respectively). No changes were detected in the PPV group. A strong association among the magnitude of LPRs to CRM197 at week 4, the breadth of SPP specific IgG responses at week 8 (P = .03), and sustained IgG responses at week 24 was observed. A high frequency of helper follicular CD4+CXCR5+ T cells at baseline was associated with a better LPR response to CRM197. Conclusions. The PCV prime-elicited memory T cell responses were associated with better and sustained humoral SPP specific IgG responses. ClinicalTrials.gov identifier. NCT00148824.