Assessing collagen alterations in enzymatic degradation models of osteoarthritis via second harmonic generation microscopy

• Published in: Osteoarthritis and cartilage Vol. 29; no. 11; pp. 1590 - 1599
• Main Authors: Jambor, A.N., Shelton, E.M., Kijowski, R., Henak, C.R., Campagnola, P.J.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.11.2021
• Subjects: Animals; Cartilage, Articular - pathology; Cattle; Collagen; Collagenases; Humans; Osteoarthritis; Osteoarthritis - pathology; Patella - pathology; Scattering; Second harmonic generation; Second Harmonic Generation Microscopy; Trypsin; Second harmonic generation; Osteoarthritis; Collagen; Scattering

Structural changes in the collagen II architecture of osteoarthritis (OA) are poorly understood, which is a large shortcoming in the early diagnosis of this disease. Though degradation can be simulated by enzymes including trypsin and bacterial collagenase, the specific structural features of each d...