Immunological evaluation of urinary trypsin inhibitors in blood and urine: Role of N- & O-linked glycoproteins

• Published in: Glycoconjugate journal Vol. 24; no. 1; pp. 5 - 15
• Main Authors: Pugia, Michael J., Jortani, Saeed A., Basu, Manju, Sommer, Ronald, Kuo, Hai-Hang, Murphy, Solomon, Williamson, Doug, Vranish, James, Boyle, Patrick J., Budzinski, Danny, Valdes, Roland, Basu, Subhash C.
• Format: Journal Article
• Language: English
• Published: United States Springer Nature B.V 01.01.2007
• Subjects: Animals; Antibodies, Monoclonal - analysis; Antibodies, Monoclonal - chemistry; Antibodies, Monoclonal - metabolism; Antibody Affinity; Blood; Cross Reactions; Enzyme-Linked Immunosorbent Assay; Glycoproteins - blood; Glycoproteins - chemistry; Glycoproteins - immunology; Glycoproteins - urine; Humans; Mass spectrometry; Mice; Mice, Inbred BALB C; Plasma; Proteins; Reference Standards; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Trypsin Inhibitors - blood; Trypsin Inhibitors - immunology; Trypsin Inhibitors - urine

Urinary trypsin inhibitors (uTi) suppress serine proteases during inflammation. After liberation from proinhibitors (P-alpha-I and I-alpha-I) by the white blood cell (WBC) response, uTi readily pass through the kidneys into urine. A key uTi, bikunin, is attached to O-linked and N-linked glycoconjuga...