CRISPR/Cas9 mediated G4946E substitution in the ryanodine receptor of Spodoptera exigua confers high levels of resistance to diamide insecticides

• Published in: Insect biochemistry and molecular biology Vol. 89; pp. 79 - 85
• Main Authors: Zuo, Yayun, Wang, Hui, Xu, Yanjun, Huang, Jianlei, Wu, Shuwen, Wu, Yidong, Yang, Yihua
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.10.2017
• Subjects: Amino Acid Substitution; Animals; Beet armyworm; calcium; Chilo suppressalis; CRISPR-Cas Systems; CRISPR/Cas9; cyantraniliprole; death; Diamide; Drosophila melanogaster; Female; flubendiamide; gene editing; Gene Editing - methods; genetic background; homozygosity; Insect Control - methods; Insecticide Resistance - genetics; Insecticides; insects; ions; Male; muscle contraction; mutation; paralysis; pests; Plutella xylostella; Point Mutation; Resistance; Ryanodine receptor; Ryanodine Receptor Calcium Release Channel - genetics; ryanodine receptors; Spodoptera - genetics; Spodoptera exigua; Tuta absoluta; Resistance; Beet armyworm; CRISPR/Cas9; Ryanodine receptor; Diamide

Diamide insecticides selectively activate insect ryanodine receptors (RyRs), inducing uncontrolled release of calcium ions, and causing muscle contraction, paralysis and eventually death. The RyRG4946E substitution associated with diamide resistance has been identified in three lepidopteran pests, P...