Influenza A Virus Transmission Bottlenecks Are Defined by Infection Route and Recipient Host

• Published in: Cell host & microbe Vol. 16; no. 5; pp. 691 - 700
• Main Authors: Varble, Andrew, Albrecht, Randy A., Backes, Simone, Crumiller, Marshall, Bouvier, Nicole M., Sachs, David, García-Sastre, Adolfo, tenOever, Benjamin R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 12.11.2014
• Subjects: Animals; Cell Line, Tumor; Disease Transmission, Infectious - veterinary; DNA Barcoding, Taxonomic; Dogs; Ferrets; Genome, Viral; Guinea Pigs; High-Throughput Nucleotide Sequencing; Host-Pathogen Interactions; Humans; Influenza A virus - genetics; Influenza A virus - pathogenicity; Influenza, Human - transmission; Madin Darby Canine Kidney Cells; Male; Orthomyxoviridae Infections - transmission

Despite its global relevance, our understanding of how influenza A virus transmission impacts the overall population dynamics of this RNA virus remains incomplete. To define this dynamic, we inserted neutral barcodes into the influenza A virus genome to generate a population of viruses that can be i...