A CRISPR/Cas13a-powered catalytic electrochemical biosensor for successive and highly sensitive RNA diagnostics

• Published in: Biosensors & bioelectronics Vol. 178; p. 113027
• Main Authors: Sheng, Yan, Zhang, Tenghua, Zhang, Shihong, Johnston, Midori, Zheng, Xiaohe, Shan, Yuanyue, Liu, Tong, Huang, Zena, Qian, Feiyang, Xie, Zihui, Ai, Yiru, Zhong, Hankang, Kuang, Tairong, Dincer, Can, Urban, Gerald Anton, Hu, Jiaming
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.04.2021
• Subjects: Biosensing Techniques; biosensors; blood serum; Carcinoma, Non-Small-Cell Lung; Catalytic hairpin; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR/Cas technology; detection limit; diagnostic techniques; DNA; DNA circuit; DNA, Catalytic; Electrochemical analysis; electrochemistry; Humans; lung neoplasms; Lung Neoplasms - diagnosis; Lung Neoplasms - genetics; Nucleic acid diagnostics; On-site testing; processing time; voltammetry; DNA circuit; Electrochemical analysis; On-site testing; Catalytic hairpin; Nucleic acid diagnostics; CRISPR/Cas technology

Rapid and specific quantitation of a variety of RNAs with low expression levels in early-stage cancer is highly desirable but remains a challenge. Here, we present a dual signal amplification strategy consisting of the CRISPR/Cas13a system and a catalytic hairpin DNA circuit (CHDC), integrated on a...