Identification of testis development and spermatogenesis-related genes in human and mouse testes using cDNA arrays
We have constructed cDNA microarrays from the human testis large insert cDNA library, containing 9216 genes, together with several housekeeping genes. The cDNA microarrays were used to identify gene expression differences between human fetal and adult testes. Of >8700 hybridized clones, 731 exhib...
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Published in | Molecular human reproduction Vol. 8; no. 6; pp. 511 - 517 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Oxford University Press
01.06.2002
Oxford Publishing Limited (England) |
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Abstract | We have constructed cDNA microarrays from the human testis large insert cDNA library, containing 9216 genes, together with several housekeeping genes. The cDNA microarrays were used to identify gene expression differences between human fetal and adult testes. Of >8700 hybridized clones, 731 exhibited significant differential expression characteristics. About 7500 genes were identified when the same cDNA microarrays were used for hybridization with cDNA probes from mouse testis, with 256 genes having significant differential expression between the age of 1–4 weeks. Among these genes, 101 were identified as critically related to testis development and possibly to spermatogenesis since they were found in both human and mouse testes, and expressed differentially at different stages of testis development. Of the 101 development-related genes, 59 full-length cDNAs have been sequenced previously, while the full-length cDNAs of the other 42 genes have not been published. We have obtained 11 full-length sequences of the 42 genes and deposited them in the GenBank. The conserved testis development-related genes found in both human and mouse testes may include genes that are likely to be involved in testicular functions, especially spermatogenesis, thus providing a basis for further functional characterization of the genes in mouse models. |
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AbstractList | We have constructed cDNA microarrays from the human testis large insert cDNA library, containing 9216 genes, together with several housekeeping genes. The cDNA microarrays were used to identify gene expression differences between human fetal and adult testes. Of >8700 hybridized clones, 731 exhibited significant differential expression characteristics. About 7500 genes were identified when the same cDNA microarrays were used for hybridization with cDNA probes from mouse testis, with 256 genes having significant differential expression between the age of 1-4 weeks. Among these genes, 101 were identified as critically related to testis development and possibly to spermatogenesis since they were found in both human and mouse testes, and expressed differentially at different stages of testis development. Of the 101 development-related genes, 59 full-length cDNAs have been sequenced previously, while the full-length cDNAs of the other 42 genes have not been published. We have obtained 11 full-length sequences of the 42 genes and deposited them in the GenBank. The conserved testis development-related genes found in both human and mouse testes may include genes that are likely to be involved in testicular functions, especially spermatogenesis, thus providing a basis for further functional characterization of the genes in mouse models. We have constructed cDNA microarrays from the human testis large insert cDNA library, containing 9216 genes, together with several housekeeping genes. The cDNA microarrays were used to identify gene expression differences between human fetal and adult testes. Of >8700 hybridized clones, 731 exhibited significant differential expression characteristics. About 7500 genes were identified when the same cDNA microarrays were used for hybridization with cDNA probes from mouse testis, with 256 genes having significant differential expression between the age of 1-4 weeks. Among these genes, 101 were identified as critically related to testis development and possibly to spermatogenesis since they were found in both human and mouse testes, and expressed differentially at different stages of testis development. Of the 101 development-related genes, 59 full-length cDNAs have been sequenced previously, while the full-length cDNAs of the other 42 genes have not been published. We have obtained 11 full-length sequences of the 42 genes and deposited them in the GenBank. The conserved testis development-related genes found in both human and mouse testes may include genes that are likely to be involved in testicular functions, especially spermatogenesis, thus providing a basis for further functional characterization of the genes in mouse models. |
Author | Li, Jianmin Sha, Jiahao Luo, Ming Zhou, Zuomin Yang, Huanmin Yin, Lanlan Hu, Gengxi Zhou, Kaiya Chan, Hsiao Chang |
Author_xml | – sequence: 1 givenname: Jiahao surname: Sha fullname: Sha, Jiahao organization: Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 210029 – sequence: 2 givenname: Zuomin surname: Zhou fullname: Zhou, Zuomin organization: Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 210029 – sequence: 3 givenname: Jianmin surname: Li fullname: Li, Jianmin organization: Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 210029 – sequence: 4 givenname: Lanlan surname: Yin fullname: Yin, Lanlan organization: Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 210029 – sequence: 5 givenname: Huanmin surname: Yang fullname: Yang, Huanmin organization: Institute of Genetics, Chinese Academy of Sciences, Shanghai, 101300 – sequence: 6 givenname: Gengxi surname: Hu fullname: Hu, Gengxi organization: Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai, 200031, P.R.China – sequence: 7 givenname: Ming surname: Luo fullname: Luo, Ming organization: Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA – sequence: 8 givenname: Hsiao Chang surname: Chan fullname: Chan, Hsiao Chang organization: Department of physiology, The Chinese University of Hong Kong, 2001 H.K. and – sequence: 9 givenname: Kaiya surname: Zhou fullname: Zhou, Kaiya organization: Institute of genetic resources, Nanjing Normal University, Nanjing, 210097, P.R.China |
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Keywords | Human Molecular hybridization Spermatogenesis Rodentia Gene expression Testicle Male genital system Plasmid Vertebrata Mammalia Complementary DNA Mouse Development |
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SubjectTerms | Adult Animals Biological and medical sciences cDNA array development Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental Humans Male Mammalian male genital system Mice Molecular Sequence Data Morphology. Physiology Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis RNA, Messenger - genetics RNA, Messenger - metabolism spermatogenesis Spermatogenesis - genetics Spermatogenesis - physiology testis Testis - growth & development Testis - physiology Vertebrates: reproduction |
Title | Identification of testis development and spermatogenesis-related genes in human and mouse testes using cDNA arrays |
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