Establishment and characterization of stable, diverse, fecal-derived in vitro microbial communities that model the intestinal microbiota

• Published in: Cell host & microbe Vol. 30; no. 2; pp. 260 - 272.e5
• Main Authors: Aranda-Díaz, Andrés, Ng, Katharine Michelle, Thomsen, Tani, Real-Ramírez, Imperio, Dahan, Dylan, Dittmar, Susannah, Gonzalez, Carlos Gutierrez, Chavez, Taylor, Vasquez, Kimberly S., Nguyen, Taylor H., Yu, Feiqiao Brian, Higginbottom, Steven K., Neff, Norma F., Elias, Joshua E., Sonnenburg, Justin L., Huang, Kerwyn Casey
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 09.02.2022
• Subjects: Animals; antibiotics; Bacteria; Bacteroides; ciprofloxacin; culturomics; ecological stability; ex vivo; Feces - microbiology; Gastrointestinal Microbiome; gut microbiota; Humans; Mice; microbial ecology; Microbiota; microbiota perturbations; synthetic communities; microbiota perturbations; ecological stability; microbial ecology; ex vivo; synthetic communities; antibiotics; culturomics; gut microbiota; ciprofloxacin

Efforts to probe the role of the gut microbiota in disease would benefit from a system in which patient-derived bacterial communities can be studied at scale. We addressed this by validating a strategy to propagate phylogenetically complex, diverse, stable, and highly reproducible stool-derived comm...