Programmed Secretion Arrest and Receptor-Triggered Toxin Export during Antibacterial Contact-Dependent Growth Inhibition

• Published in: Cell Vol. 175; no. 4; pp. 921 - 933.e14
• Main Authors: Ruhe, Zachary C., Subramanian, Poorna, Song, Kiho, Nguyen, Josephine Y., Stevens, Taylor A., Low, David A., Jensen, Grant J., Hayes, Christopher S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2018
• Subjects: Antibiosis; bacterial competition; BamA; Cell Surface Extensions - metabolism; Cell Surface Extensions - ultrastructure; Escherichia coli - metabolism; Escherichia coli Proteins - chemistry; Escherichia coli Proteins - metabolism; Membrane Proteins - chemistry; Membrane Proteins - metabolism; outer membrane; Protein Domains; Receptors, Cell Surface - metabolism; self-nonself discrimination; toxin-immunity proteins; Tsx; two-partner secretion; type V secretion system; Type V Secretion Systems - metabolism; β-barrel protein; outer membrane; two-partner secretion; self-nonself discrimination; toxin-immunity proteins; bacterial competition; Tsx; BamA; type V secretion system; β-barrel protein

Contact-dependent growth inhibition (CDI) entails receptor-mediated delivery of CdiA-derived toxins into Gram-negative target bacteria. Using electron cryotomography, we show that each CdiA effector protein forms a filament extending ∼33 nm from the cell surface. Remarkably, the extracellular filame...