Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice

• Published in: Plant science (Limerick) Vol. 163; no. 3; pp. 515 - 523
• Main Authors: Uchida, Akio, Jagendorf, Andre T, Hibino, Takashi, Takabe, Teruhiro, Takabe, Tetsuko
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.09.2002; Elsevier Science
• Subjects: Abiotic stress tolerance; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; H 2O 2; Heat stress; Metabolism; Metabolism. Physicochemical requirements; Nitric oxide; Plant physiology and development; Salt stress; SNP, sodium nitroprusside; Salt stress; Abiotic stress tolerance; HSP26, small heat shock protein 26; P5CS, Δ′-pyrroline-5-carboxylate synthase; Cat, catalase; SPS, sucrose-phosphate synthase; PAM, pulse amplitude modulated fluorometer; SOD, superoxide dismutase; H 2O 2; NO, nitric oxide; Nitric oxide; GR, glutathione reductase; AOS, active oxygen scavenging; POX, guaiacol peroxidase; Heat stress; APX, ascorbate peroxidase; Monocotyledones; Enzyme; Transferases; Glycosyltransferases; Tolerance; Gene expression; Sucrose-phosphate synthase; Hydrogen Peroxides; Survival; Cereal crop; Salinity; Oryza sativa; Signal transduction; Gramineae; Thermal shock; Angiospermae; Heat shock protein; Hexosyltransferases; Spermatophyta; Pretreatment
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/S0168-9452(02)00159-0

Higher plants growing in natural environments experience various abiotic stresses. H 2O 2 and nitric oxide (NO) free radicals are produced and cause oxidative damage to plants under various abiotic stress conditions. However, in the present study, we found that pretreating rice seedlings with low levels (<10 μM) of H 2O 2 or NO permitted the survival of more green leaf tissue, and of higher quantum yield for photosystem II, than in non-treated controls, under salt and heat stresses. It was also shown that the pretreatment induces not only active oxygen scavenging enzymes activities, but also expression of transcripts for stress-related genes encoding sucrose-phosphate synthase, Δ′-pyrroline-5-carboxylate synthase, and small heat shock protein 26, These results suggest that H 2O 2 and NO can increase both salt and heat tolerance in rice seedlings by acting as signal molecules for the response.