Synthesis of Mesoporous Silica Coated Gold Nanorods Loaded with Methylene Blue and Its Potentials in Antibacterial Applications

• Published in: Nanomaterials (Basel, Switzerland) Vol. 11; no. 5; p. 1338
• Main Authors: Fernández-Lodeiro, Adrián, Djafari, Jamila, Fernández-Lodeiro, Javier, Duarte, Maria Paula, Muchagato Mauricio, Elisabete, Capelo-Martínez, José Luis, Lodeiro, Carlos
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 19.05.2021; MDPI
• Subjects: antibacterial activity; Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; Bacteria; Cancer; Coliforms; Conjugation; Cryoforming; Cytotoxicity; E coli; Fourier transforms; Freeze drying; Gold; gold nanoparticles; gold nanorod; Gram-positive bacteria; Irradiation; Lasers; mesoporous silica; Methylene blue; Nanorods; Photodegradation; Photodynamic therapy; Scanning electron microscopy; Silica; Silicon dioxide; Spectrum analysis; Trehalose; Ultrasound; Japan; Germany
• ISSN: 2079-4991; 2079-4991
• DOI: 10.3390/nano11051338

In this work, the successful preparation and characterization of gold nanorods (AuNRs) coated with a mesoporous silica shell (AuNRs@Simes) was achieved. Conjugation with methylene blue (MB) as a model drug using ultrasound-stimulated loading has been explored for further application in light-mediated antibacterial studies. Lyophilization of this conjugated nanosystem was analyzed using trehalose (TRH) as a cryogenic protector. The obtained stable dry formulation shows potent antimicrobial activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria after a simple post-treatment irradiation method with a red laser during a short time period.