Analysis of cytokine gene expression during fetal thymic ontogeny using the polymerase chain reaction

• Published in: The Journal of immunology (1950) Vol. 147; no. 2; pp. 554 - 560
• Main Authors: Montgomery, RA, Dallman, MJ
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 15.07.1991; American Association of Immunologists
• Subjects: Animals; Base Sequence; Biological and medical sciences; Cytokines - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression; Gestational Age; Heart - embryology; Interleukin-2 - genetics; Interleukin-7 - genetics; Liver - embryology; Mice; Mice, Inbred BALB C; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Oligonucleotides - chemistry; Polymerase Chain Reaction; Receptors, Cell Surface - genetics; Receptors, Interleukin-2 - genetics; Thymus Gland - embryology; Thymus gland; Thymocyte; Rodentia; Cytokine; Gene expression; Northern blotting; Polymerase chain reaction; Vertebrata; Mammalia; Gene; Mouse; T-Lymphocyte; Development; Fetus

The expression of 12 cytokine and cytokine receptor genes during mouse thymic ontogeny has been studied using the polymerase chain reaction. The data reveal that IL-2 gene transcription does not begin in the thymus until fetal day (Fd) 16. However, IL-2R beta-chain mRNA was detectable throughout the...