Nanostructured biosensor for detecting glucose in tear by applying fluorescence resonance energy transfer quenching mechanism

• Published in: Biosensors & bioelectronics Vol. 91; pp. 393 - 399
• Main Authors: Chen, Longyi, Tse, Wai Hei, Chen, Yi, McDonald, Matthew W., Melling, James, Zhang, Jin
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.05.2017
• Subjects: Animals; Biosensing Techniques - methods; biosensors; blood glucose; Blood Glucose - analysis; Cadmium Compounds - chemistry; calibration; Canavalia - chemistry; Coloring Agents - chemistry; concanavalin A; Concanavalin A - chemistry; dextran; Dextrans - chemistry; energy transfer; fluorescence; fluorescence emission spectroscopy; Fluorescence resonance energy transfer (FRET); Fluorescence Resonance Energy Transfer - methods; glucose; Glucose - analysis; Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry; hydrogels; malachite green; Male; males; Models, Molecular; nanorods; Nanotubes - chemistry; Nanotubes - ultrastructure; Non-invasive biosensor; photoluminescence; Quantum dots; Quantum Dots - chemistry; Quantum Dots - ultrastructure; rats; Rats, Sprague-Dawley; Rosaniline Dyes - chemistry; Selenium Compounds - chemistry; Signal-To-Noise Ratio; Silicon - chemistry; silicone; Silicone hydrogel; Sulfides - chemistry; Tear glucose; Tears - chemistry; Zinc Compounds - chemistry; zinc oxide; Zinc Oxide - chemistry; zinc sulfide; Silicone hydrogel; Non-invasive biosensor; Tear glucose; Quantum dots; Fluorescence resonance energy transfer (FRET)

In this paper, a nanostructured biosensor is developed to detect glucose in tear by using fluorescence resonance energy transfer (FRET) quenching mechanism. The designed FRET pair, including the donor, CdSe/ZnS quantum dots (QDs), and the acceptor, dextran-binding malachite green (MG-dextran), was c...