Cellular Therapy Using Epitope-Imprinted Composite Nanoparticles to Remove α-Synuclein from an In Vitro Model

Several degenerative disorders of the central nervous system, including Parkinson’s disease (PD), are related to the pathological aggregation of proteins. Antibodies against toxic disease proteins, such as α-synuclein (SNCA), are therefore being developed as possible therapeutics. In this work, one...

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Published inCells (Basel, Switzerland) Vol. 11; no. 16; p. 2584
Main Authors Lee, Mei-Hwa, Jan, Jeng-Shiung, Thomas, James L, Shih, Yuan-Pin, Li, Jin-An, Lin, Chien-Yu, Ooya, Tooru, Barna, Lilla, Mészáros, Mária, Harazin, András, Porkoláb, Gergő, Veszelka, Szilvia, Deli, Maria A, Lin, Hung-Yin
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.08.2022
MDPI
Subjects
Adsorption
Alzheimer's disease
Antibodies
Antigenic determinants
Binding sites
Brain
Calorimetry
Cell therapy
Cellular therapy
Central nervous system
Communication
CRISPR
Dementia
Endothelial cells
Epitopes
gene activation
Health aspects
High-performance liquid chromatography
Light scattering
magnetic nanoparticles
Membrane proteins
Methods
Movement disorders
Nanoparticles
Neurodegenerative diseases
Parkinson's disease
peptide imprinting
Peptides
protein extraction
Proteins
Quantum dots
Synuclein
Titration
α-synuclein
Taiwan
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Abstract Several degenerative disorders of the central nervous system, including Parkinson’s disease (PD), are related to the pathological aggregation of proteins. Antibodies against toxic disease proteins, such as α-synuclein (SNCA), are therefore being developed as possible therapeutics. In this work, one peptide (YVGSKTKEGVVHGVA) from SNCA was used as the epitope to construct magnetic molecularly imprinted composite nanoparticles (MMIPs). These composite nanoparticles were characterized by dynamic light scattering (DLS), high-performance liquid chromatography (HPLC), isothermal titration calorimetry (ITC), Brunauer–Emmett–Teller (BET) analysis, and superconducting quantum interference device (SQUID) analysis. Finally, the viability of brain endothelial cells that were treated with MMIPs was measured, and the extraction of SNCA from CRISPR/dCas9a-activated HEK293T cells from the in vitro model system was demonstrated for the therapeutic application of MMIPs.
AbstractList Several degenerative disorders of the central nervous system, including Parkinson’s disease (PD), are related to the pathological aggregation of proteins. Antibodies against toxic disease proteins, such as α-synuclein (SNCA), are therefore being developed as possible therapeutics. In this work, one peptide (YVGSKTKEGVVHGVA) from SNCA was used as the epitope to construct magnetic molecularly imprinted composite nanoparticles (MMIPs). These composite nanoparticles were characterized by dynamic light scattering (DLS), high-performance liquid chromatography (HPLC), isothermal titration calorimetry (ITC), Brunauer–Emmett–Teller (BET) analysis, and superconducting quantum interference device (SQUID) analysis. Finally, the viability of brain endothelial cells that were treated with MMIPs was measured, and the extraction of SNCA from CRISPR/dCas9a-activated HEK293T cells from the in vitro model system was demonstrated for the therapeutic application of MMIPs.
Audience Academic
Author Deli, Maria A
Lin, Hung-Yin
Thomas, James L
Lee, Mei-Hwa
Jan, Jeng-Shiung
Li, Jin-An
Porkoláb, Gergő
Ooya, Tooru
Mészáros, Mária
Harazin, András
Shih, Yuan-Pin
Veszelka, Szilvia
Barna, Lilla
Lin, Chien-Yu
AuthorAffiliation 2 Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan
6 Center for Advanced Medical Engineering Research & Development (CAMED), Kobe University, Kobe 657-8501, Japan
7 Institute of Biophysics, Biological Research Centre, H-6726 Szeged, Hungary
3 Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, USA
1 Department of Materials Science and Engineering, I-Shou University, Kaohsiung 84001, Taiwan
5 Graduate School of Engineering, Department of Chemical Science and Engineering, Kobe University, Kobe 657-8501, Japan
8 Doctoral School in Biology, University of Szeged, H-6720 Szeged, Hungary
4 Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan
AuthorAffiliation_xml – name: 3 Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, USA
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– name: 4 Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan
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CitedBy_id crossref_primary_10_3390_molecules28030918
crossref_primary_10_3390_bios13030357
crossref_primary_10_1016_j_microc_2023_108931
crossref_primary_10_3390_polym15214199
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Snippet Several degenerative disorders of the central nervous system, including Parkinson’s disease (PD), are related to the pathological aggregation of proteins....
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SubjectTerms Adsorption
Alzheimer's disease
Antibodies
Antigenic determinants
Binding sites
Brain
Calorimetry
Cell therapy
Cellular therapy
Central nervous system
Communication
CRISPR
Dementia
Endothelial cells
Epitopes
gene activation
Health aspects
High-performance liquid chromatography
Light scattering
magnetic nanoparticles
Membrane proteins
Methods
Movement disorders
Nanoparticles
Neurodegenerative diseases
Parkinson's disease
peptide imprinting
Peptides
protein extraction
Proteins
Quantum dots
Synuclein
Titration
α-synuclein
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Title Cellular Therapy Using Epitope-Imprinted Composite Nanoparticles to Remove α-Synuclein from an In Vitro Model
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Volume 11
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