Chimeric flavivirus causes vascular leakage and bone marrow suppression in a mouse model

Previously, we demonstrated the utility of a recombinant chimeric flavivirus (DV2ChimV), which carries the premembrane (prM) and envelope (E) genes of a type 2 DENV clinical (Thai) isolate on a backbone of Japanese encephalitis virus, for evaluating the protective efficacy of antidengue envelope ant...

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Published in	Biochemical and biophysical research communications Vol. 659; pp. 54 - 61
Main Authors	Kurosu, Takeshi, Hanabara, Keiko, Asai, Azusa, Pambudi, Sabar, Phanthanawiboon, Supranee, Omokoko, Magot Diata, Sakai, Yusuke, Suzuki, Tadaki, Ikuta, Kazuyoshi
Format	Journal Article
Language	English
Published	United States Elsevier Inc 04.06.2023
Subjects	Animals Antibodies, Viral bone marrow Bone Marrow - pathology Bone marrow suppression Dengue Dengue Virus Flavivirus Hemorrhagic fever Humans intestines Japanese encephalitis virus liver megakaryocytes Mice Mice, Knockout Mouse model Vascular leakage viruses Bone marrow suppression Hemorrhagic fever Mouse model Dengue virus Vascular leakage Flavivirus
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Abstract	Previously, we demonstrated the utility of a recombinant chimeric flavivirus (DV2ChimV), which carries the premembrane (prM) and envelope (E) genes of a type 2 DENV clinical (Thai) isolate on a backbone of Japanese encephalitis virus, for evaluating the protective efficacy of antidengue envelope antibodies both in vitro and in vivo. Here, to assess the potential use of this model for pathological studies, we aimed to characterize interferon-α/β–γ-receptor double-knockout mice (IFN-α/β/γR dKO mice) infected with DV2ChimV. Vascular leakage and bone marrow suppression are unique features of severe dengue. In the current model, DV2ChimV caused vascular leakage in the liver and intestine at the moribund stage. High levels of virus were detected in the bone marrow, and strong bone marrow suppression (i.e., disappearance of megakaryocytes and erythroblastic islets) was observed. These observations suggest that the DV2ChimV-infected mouse model mimics the vascular leakage and bone marrow suppression observed in human cases. •DV2ChimV causes vascular leakage in the liver and intestine.•DV2ChimV causes strong bone marrow suppression in mice.•Megakaryocytes and erythroblastic islets disappeared from infected mice.•Blockade of TNF-α partly protected mice from lethal infection.
AbstractList	Previously, we demonstrated the utility of a recombinant chimeric flavivirus (DV2ChimV), which carries the premembrane (prM) and envelope (E) genes of a type 2 DENV clinical (Thai) isolate on a backbone of Japanese encephalitis virus, for evaluating the protective efficacy of antidengue envelope antibodies both in vitro and in vivo. Here, to assess the potential use of this model for pathological studies, we aimed to characterize interferon-α/β–γ-receptor double-knockout mice (IFN-α/β/γR dKO mice) infected with DV2ChimV. Vascular leakage and bone marrow suppression are unique features of severe dengue. In the current model, DV2ChimV caused vascular leakage in the liver and intestine at the moribund stage. High levels of virus were detected in the bone marrow, and strong bone marrow suppression (i.e., disappearance of megakaryocytes and erythroblastic islets) was observed. These observations suggest that the DV2ChimV-infected mouse model mimics the vascular leakage and bone marrow suppression observed in human cases. •DV2ChimV causes vascular leakage in the liver and intestine.•DV2ChimV causes strong bone marrow suppression in mice.•Megakaryocytes and erythroblastic islets disappeared from infected mice.•Blockade of TNF-α partly protected mice from lethal infection. Previously, we demonstrated the utility of a recombinant chimeric flavivirus (DV2ChimV), which carries the premembrane (prM) and envelope (E) genes of a type 2 DENV clinical (Thai) isolate on a backbone of Japanese encephalitis virus, for evaluating the protective efficacy of antidengue envelope antibodies both in vitro and in vivo. Here, to assess the potential use of this model for pathological studies, we aimed to characterize interferon-α/β-γ-receptor double-knockout mice (IFN-α/β/γR dKO mice) infected with DV2ChimV. Vascular leakage and bone marrow suppression are unique features of severe dengue. In the current model, DV2ChimV caused vascular leakage in the liver and intestine at the moribund stage. High levels of virus were detected in the bone marrow, and strong bone marrow suppression (i.e., disappearance of megakaryocytes and erythroblastic islets) was observed. These observations suggest that the DV2ChimV-infected mouse model mimics the vascular leakage and bone marrow suppression observed in human cases. Previously, we demonstrated the utility of a recombinant chimeric flavivirus (DV2ChimV), which carries the premembrane (prM) and envelope (E) genes of a type 2 DENV clinical (Thai) isolate on a backbone of Japanese encephalitis virus, for evaluating the protective efficacy of antidengue envelope antibodies both in vitro and in vivo. Here, to assess the potential use of this model for pathological studies, we aimed to characterize interferon-α/β-γ-receptor double-knockout mice (IFN-α/β/γR dKO mice) infected with DV2ChimV. Vascular leakage and bone marrow suppression are unique features of severe dengue. In the current model, DV2ChimV caused vascular leakage in the liver and intestine at the moribund stage. High levels of virus were detected in the bone marrow, and strong bone marrow suppression (i.e., disappearance of megakaryocytes and erythroblastic islets) was observed. These observations suggest that the DV2ChimV-infected mouse model mimics the vascular leakage and bone marrow suppression observed in human cases.Previously, we demonstrated the utility of a recombinant chimeric flavivirus (DV2ChimV), which carries the premembrane (prM) and envelope (E) genes of a type 2 DENV clinical (Thai) isolate on a backbone of Japanese encephalitis virus, for evaluating the protective efficacy of antidengue envelope antibodies both in vitro and in vivo. Here, to assess the potential use of this model for pathological studies, we aimed to characterize interferon-α/β-γ-receptor double-knockout mice (IFN-α/β/γR dKO mice) infected with DV2ChimV. Vascular leakage and bone marrow suppression are unique features of severe dengue. In the current model, DV2ChimV caused vascular leakage in the liver and intestine at the moribund stage. High levels of virus were detected in the bone marrow, and strong bone marrow suppression (i.e., disappearance of megakaryocytes and erythroblastic islets) was observed. These observations suggest that the DV2ChimV-infected mouse model mimics the vascular leakage and bone marrow suppression observed in human cases. Previously, we demonstrated the utility of a recombinant chimeric flavivirus (DV2ChimV), which carries the premembrane (prM) and envelope (E) genes of a type 2 DENV clinical (Thai) isolate on a backbone of Japanese encephalitis virus, for evaluating the protective efficacy of antidengue envelope antibodies both in vitro and in vivo. Here, to assess the potential use of this model for pathological studies, we aimed to characterize interferon-α/β-γ-receptor double-knockout mice (IFN-α/β/γR dKO mice) infected with DV2ChimV. Vascular leakage and bone marrow suppression are unique features of severe dengue. In the current model, DV2ChimV caused vascular leakage in the liver and intestine at the moribund stage. High levels of virus were detected in the bone marrow, and strong bone marrow suppression (i.e., disappearance of megakaryocytes and erythroblastic islets) was observed. These observations suggest that the DV2ChimV-infected mouse model mimics the vascular leakage and bone marrow suppression observed in human cases.
Author	Hanabara, Keiko Phanthanawiboon, Supranee Suzuki, Tadaki Pambudi, Sabar Omokoko, Magot Diata Ikuta, Kazuyoshi Sakai, Yusuke Kurosu, Takeshi Asai, Azusa
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Keywords	Bone marrow suppression Hemorrhagic fever Mouse model Dengue virus Vascular leakage Flavivirus
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References	Guzman, Gubler, Izquierdo, Martinez, Halstead (bib8) 2016; 2 Albuquerque, Silva Junior, Diogenes, Silva (bib28) 2009; 7 Nelson, Bierman, Chulajata (bib13) 1966; 252 Mitrakul, Poshyachinda, Futrakul, Sangkawibha, Ahandrik (bib21) 1977; 26 Mackenzie, Gubler, Petersen (bib1) 2004; 10 Kurosu, Khamlert, Phanthanawiboon, Ikuta, Anantapreecha (bib16) 2010; 394 Sampath, Padmanabhan (bib2) 2009; 81 Sarathy, Milligan, Bourne, Barrett (bib23) 2015; 33 Basler (bib7) 2017; 39 Na-Nakorn, Suingdumrong, Pootrakul, Bhamarapravati (bib12) 1966; 35 Srichaikul, Nimmannitya (bib22) 2000; 13 Rothman (bib18) 2011; 11 Noisakran, Onlamoon, Hsiao, Clark, Villinger, Ansari, Perng (bib10) 2012; 40 Shresta, Sharar, Prigozhin, Beatty, Harris (bib24) 2006; 80 Phanthanawiboon, Limkittikul, Sakai, Takakura, Saijo, Kurosu (bib17) 2016; 11 Bierman, Nelson (bib11) 1965; 62 Pugachev, Schwaiger, Brown, Zhang, Catalan, Mitchell, Ocran, Rumyantsev, Khromykh, Monath, Guirakhoo (bib5) 2007; 25 Watanabe, Chan, Wang, Rivino, Lok, Vasudevan (bib25) 2015; 89 Pradeep, Hoovina Venkatesh, Manchala, Vayal Veedu, Basavaraju, Selvasundari, Ramakrishna, Chandrakiran, Krishnamurthy, Holigi, Thomas, Ross, Dias, Satchidanandam (bib29) 2021; 12 La Russa, Innis (bib9) 1995; 8 Arora, Nandan, Sharma, Benerjee, Singh (bib27) 2021; 58 Vogt, Lahon, Arya, Spencer Clinton, Rico-Hesse (bib14) 2019; 13 Guzman, Harris (bib19) 2015; 385 Chambers, Jiang, Droll, Liang, Wold, Nickells (bib3) 2006; 87 Pripuzova, Tereshkina, Gmyl, Dzhivanyan, Rumyantsev, Romanova, Mustafina, Lashkevich, Karganova (bib4) 2009; 81 Kurosu, Hanabara, Asai, Pambudi, Phanthanawiboon, Omokoko, Ono, Saijo, Ramasoota, Ikuta (bib15) 2020; 10 Dimaano, Saito, Honda, Miranda, Alonzo, Valerio, Mapua, Inoue, Kumaori, Matias, Natividad, Oishi (bib20) 2007; 77 de Azeredo, Monteiro, de-Oliveira Pinto (bib26) 2015; 2015 Pierson, Diamond (bib6) 2020; 5 de Azeredo (10.1016/j.bbrc.2023.04.003_bib26) 2015; 2015 Albuquerque (10.1016/j.bbrc.2023.04.003_bib28) 2009; 7 Mackenzie (10.1016/j.bbrc.2023.04.003_bib1) 2004; 10 Sarathy (10.1016/j.bbrc.2023.04.003_bib23) 2015; 33 Arora (10.1016/j.bbrc.2023.04.003_bib27) 2021; 58 Kurosu (10.1016/j.bbrc.2023.04.003_bib15) 2020; 10 Srichaikul (10.1016/j.bbrc.2023.04.003_bib22) 2000; 13 Na-Nakorn (10.1016/j.bbrc.2023.04.003_bib12) 1966; 35 Pradeep (10.1016/j.bbrc.2023.04.003_bib29) 2021; 12 Bierman (10.1016/j.bbrc.2023.04.003_bib11) 1965; 62 Phanthanawiboon (10.1016/j.bbrc.2023.04.003_bib17) 2016; 11 Dimaano (10.1016/j.bbrc.2023.04.003_bib20) 2007; 77 Sampath (10.1016/j.bbrc.2023.04.003_bib2) 2009; 81 Mitrakul (10.1016/j.bbrc.2023.04.003_bib21) 1977; 26 Pripuzova (10.1016/j.bbrc.2023.04.003_bib4) 2009; 81 Basler (10.1016/j.bbrc.2023.04.003_bib7) 2017; 39 Chambers (10.1016/j.bbrc.2023.04.003_bib3) 2006; 87 Guzman (10.1016/j.bbrc.2023.04.003_bib8) 2016; 2 Nelson (10.1016/j.bbrc.2023.04.003_bib13) 1966; 252 Shresta (10.1016/j.bbrc.2023.04.003_bib24) 2006; 80 Pierson (10.1016/j.bbrc.2023.04.003_bib6) 2020; 5 Rothman (10.1016/j.bbrc.2023.04.003_bib18) 2011; 11 La Russa (10.1016/j.bbrc.2023.04.003_bib9) 1995; 8 Noisakran (10.1016/j.bbrc.2023.04.003_bib10) 2012; 40 Watanabe (10.1016/j.bbrc.2023.04.003_bib25) 2015; 89 Pugachev (10.1016/j.bbrc.2023.04.003_bib5) 2007; 25 Guzman (10.1016/j.bbrc.2023.04.003_bib19) 2015; 385 Vogt (10.1016/j.bbrc.2023.04.003_bib14) 2019; 13 Kurosu (10.1016/j.bbrc.2023.04.003_bib16) 2010; 394
References_xml	– volume: 8 start-page: 249 year: 1995 end-page: 270 ident: bib9 article-title: Mechanisms of dengue virus-induced bone marrow suppression publication-title: Baillieres Clin. Haematol. – volume: 394 start-page: 398 year: 2010 end-page: 404 ident: bib16 article-title: Highly efficient rescue of dengue virus using a co-culture system with mosquito/mammalian cells publication-title: Biochem. Biophys. Res. Commun. – volume: 385 start-page: 453 year: 2015 end-page: 465 ident: bib19 article-title: Dengue publication-title: Lancet – volume: 35 start-page: 54 year: 1966 end-page: 55 ident: bib12 article-title: Bone-marrow studies in Thai haemorrhagic fever publication-title: Bull. World Health Organ. – volume: 39 start-page: 551 year: 2017 end-page: 561 ident: bib7 article-title: Molecular pathogenesis of viral hemorrhagic fever publication-title: Semin. Immunopathol. – volume: 62 start-page: 867 year: 1965 end-page: 884 ident: bib11 article-title: Hematodepressive virus diseases of Thailand publication-title: Ann. Intern. Med. – volume: 25 start-page: 6661 year: 2007 end-page: 6671 ident: bib5 article-title: Construction and biological characterization of artificial recombinants between a wild type flavivirus (Kunjin) and a live chimeric flavivirus vaccine (ChimeriVax-JE) publication-title: Vaccine – volume: 33 start-page: 7051 year: 2015 end-page: 7060 ident: bib23 article-title: Mouse models of dengue virus infection for vaccine testing publication-title: Vaccine – volume: 11 year: 2016 ident: bib17 article-title: Acute systemic infection with dengue virus leads to vascular leakage and death through tumor necrosis factor-alpha and tie2/angiopoietin signaling in mice lacking type I and II interferon receptors publication-title: PLoS One – volume: 81 start-page: 6 year: 2009 end-page: 15 ident: bib2 article-title: Molecular targets for flavivirus drug discovery publication-title: Antivir. Res. – volume: 2 year: 2016 ident: bib8 article-title: Dengue infection publication-title: Nat. Rev. Dis. Prim. – volume: 7 start-page: 118 year: 2009 end-page: 120 ident: bib28 article-title: Dengue and aplastic anemia--a rare association publication-title: Trav. Med. Infect. Dis. – volume: 13 year: 2019 ident: bib14 article-title: Dengue viruses infect human megakaryocytes, with probable clinical consequences publication-title: PLoS Neglected Trop. Dis. – volume: 10 start-page: S98 year: 2004 end-page: S109 ident: bib1 article-title: Emerging flaviviruses: the spread and resurgence of Japanese encephalitis, West Nile and dengue viruses publication-title: Nat. Med. – volume: 81 start-page: 1777 year: 2009 end-page: 1785 ident: bib4 article-title: Safety evaluation of chimeric Langat/Dengue 4 flavivirus, a live vaccine candidate against tick-borne encephalitis publication-title: J. Med. Virol. – volume: 252 start-page: 68 year: 1966 end-page: 74 ident: bib13 article-title: Hematologic phagocytosis in postmortem bone marrows of dengue hemorrhagic fever. (Hematologic phagocytosis in Thai hemorrhagic fever) publication-title: Am. J. Med. Sci. – volume: 80 start-page: 10208 year: 2006 end-page: 10217 ident: bib24 article-title: Murine model for dengue virus-induced lethal disease with increased vascular permeability publication-title: J. Virol. – volume: 12 year: 2021 ident: bib29 article-title: Innate immune cytokine profiling and biomarker identification for outcome in dengue patients publication-title: Front. Immunol. – volume: 2015 year: 2015 ident: bib26 article-title: Thrombocytopenia in dengue: interrelationship between virus and the imbalance between coagulation and fibrinolysis and inflammatory mediators publication-title: Mediat. Inflamm. – volume: 40 start-page: 250 year: 2012 end-page: 259 e254 ident: bib10 article-title: Infection of bone marrow cells by dengue virus in vivo publication-title: Exp. Hematol. – volume: 87 start-page: 3131 year: 2006 end-page: 3140 ident: bib3 article-title: Chimeric Japanese encephalitis virus/dengue 2 virus infectious clone: biological properties, immunogenicity and protection against dengue encephalitis in mice publication-title: J. Gen. Virol. – volume: 11 start-page: 532 year: 2011 end-page: 543 ident: bib18 article-title: Immunity to dengue virus: a tale of original antigenic sin and tropical cytokine storms publication-title: Nat. Rev. Immunol. – volume: 77 start-page: 1135 year: 2007 end-page: 1138 ident: bib20 article-title: Lack of efficacy of high-dose intravenous immunoglobulin treatment of severe thrombocytopenia in patients with secondary dengue virus infection publication-title: Am. J. Trop. Med. Hyg. – volume: 5 start-page: 796 year: 2020 end-page: 812 ident: bib6 article-title: The continued threat of emerging flaviviruses publication-title: Nat. Microbiol. – volume: 13 start-page: 261 year: 2000 end-page: 276 ident: bib22 article-title: Haematology in dengue and dengue haemorrhagic fever publication-title: Baillieres Best Pract. Res. Clin. Haematol. – volume: 89 start-page: 5847 year: 2015 end-page: 5861 ident: bib25 article-title: Dengue virus infection with highly neutralizing levels of cross-reactive antibodies causes acute lethal small intestinal pathology without a high level of viremia in mice publication-title: J. Virol. – volume: 58 start-page: 329 year: 2021 end-page: 334 ident: bib27 article-title: Predictors of severe dengue amongst children as per the revised WHO classification publication-title: J. Vector Borne Dis. – volume: 26 start-page: 975 year: 1977 end-page: 984 ident: bib21 article-title: Hemostatic and platelet kinetic studies in dengue hemorrhagic fever publication-title: Am. J. Trop. Med. Hyg. – volume: 10 year: 2020 ident: bib15 article-title: Chimeric flavivirus enables evaluation of antibodies against dengue virus envelope protein in vitro and in vivo publication-title: Sci. Rep. – volume: 39 start-page: 551 year: 2017 ident: 10.1016/j.bbrc.2023.04.003_bib7 article-title: Molecular pathogenesis of viral hemorrhagic fever publication-title: Semin. Immunopathol. doi: 10.1007/s00281-017-0637-x – volume: 10 year: 2020 ident: 10.1016/j.bbrc.2023.04.003_bib15 article-title: Chimeric flavivirus enables evaluation of antibodies against dengue virus envelope protein in vitro and in vivo publication-title: Sci. Rep. doi: 10.1038/s41598-020-78639-x – volume: 2015 year: 2015 ident: 10.1016/j.bbrc.2023.04.003_bib26 article-title: Thrombocytopenia in dengue: interrelationship between virus and the imbalance between coagulation and fibrinolysis and inflammatory mediators publication-title: Mediat. Inflamm. doi: 10.1155/2015/313842 – volume: 77 start-page: 1135 year: 2007 ident: 10.1016/j.bbrc.2023.04.003_bib20 article-title: Lack of efficacy of high-dose intravenous immunoglobulin treatment of severe thrombocytopenia in patients with secondary dengue virus infection publication-title: Am. J. Trop. Med. Hyg. doi: 10.4269/ajtmh.2007.77.1135 – volume: 25 start-page: 6661 year: 2007 ident: 10.1016/j.bbrc.2023.04.003_bib5 article-title: Construction and biological characterization of artificial recombinants between a wild type flavivirus (Kunjin) and a live chimeric flavivirus vaccine (ChimeriVax-JE) publication-title: Vaccine doi: 10.1016/j.vaccine.2007.07.016 – volume: 80 start-page: 10208 year: 2006 ident: 10.1016/j.bbrc.2023.04.003_bib24 article-title: Murine model for dengue virus-induced lethal disease with increased vascular permeability publication-title: J. Virol. doi: 10.1128/JVI.00062-06 – volume: 7 start-page: 118 year: 2009 ident: 10.1016/j.bbrc.2023.04.003_bib28 article-title: Dengue and aplastic anemia--a rare association publication-title: Trav. Med. Infect. Dis. doi: 10.1016/j.tmaid.2009.01.001 – volume: 13 start-page: 261 year: 2000 ident: 10.1016/j.bbrc.2023.04.003_bib22 article-title: Haematology in dengue and dengue haemorrhagic fever publication-title: Baillieres Best Pract. Res. Clin. Haematol. doi: 10.1053/beha.2000.0073 – volume: 2 year: 2016 ident: 10.1016/j.bbrc.2023.04.003_bib8 article-title: Dengue infection publication-title: Nat. Rev. Dis. Prim. – volume: 35 start-page: 54 year: 1966 ident: 10.1016/j.bbrc.2023.04.003_bib12 article-title: Bone-marrow studies in Thai haemorrhagic fever publication-title: Bull. World Health Organ. – volume: 81 start-page: 1777 year: 2009 ident: 10.1016/j.bbrc.2023.04.003_bib4 article-title: Safety evaluation of chimeric Langat/Dengue 4 flavivirus, a live vaccine candidate against tick-borne encephalitis publication-title: J. Med. Virol. doi: 10.1002/jmv.21587 – volume: 13 year: 2019 ident: 10.1016/j.bbrc.2023.04.003_bib14 article-title: Dengue viruses infect human megakaryocytes, with probable clinical consequences publication-title: PLoS Neglected Trop. Dis. doi: 10.1371/journal.pntd.0007837 – volume: 87 start-page: 3131 year: 2006 ident: 10.1016/j.bbrc.2023.04.003_bib3 article-title: Chimeric Japanese encephalitis virus/dengue 2 virus infectious clone: biological properties, immunogenicity and protection against dengue encephalitis in mice publication-title: J. Gen. Virol. doi: 10.1099/vir.0.81909-0 – volume: 40 start-page: 250 year: 2012 ident: 10.1016/j.bbrc.2023.04.003_bib10 article-title: Infection of bone marrow cells by dengue virus in vivo publication-title: Exp. Hematol. doi: 10.1016/j.exphem.2011.11.011 – volume: 5 start-page: 796 year: 2020 ident: 10.1016/j.bbrc.2023.04.003_bib6 article-title: The continued threat of emerging flaviviruses publication-title: Nat. Microbiol. doi: 10.1038/s41564-020-0714-0 – volume: 8 start-page: 249 year: 1995 ident: 10.1016/j.bbrc.2023.04.003_bib9 article-title: Mechanisms of dengue virus-induced bone marrow suppression publication-title: Baillieres Clin. Haematol. doi: 10.1016/S0950-3536(05)80240-9 – volume: 394 start-page: 398 year: 2010 ident: 10.1016/j.bbrc.2023.04.003_bib16 article-title: Highly efficient rescue of dengue virus using a co-culture system with mosquito/mammalian cells publication-title: Biochem. Biophys. Res. Commun. doi: 10.1016/j.bbrc.2010.02.181 – volume: 58 start-page: 329 year: 2021 ident: 10.1016/j.bbrc.2023.04.003_bib27 article-title: Predictors of severe dengue amongst children as per the revised WHO classification publication-title: J. Vector Borne Dis. doi: 10.4103/0972-9062.318312 – volume: 11 year: 2016 ident: 10.1016/j.bbrc.2023.04.003_bib17 article-title: Acute systemic infection with dengue virus leads to vascular leakage and death through tumor necrosis factor-alpha and tie2/angiopoietin signaling in mice lacking type I and II interferon receptors publication-title: PLoS One doi: 10.1371/journal.pone.0148564 – volume: 10 start-page: S98 year: 2004 ident: 10.1016/j.bbrc.2023.04.003_bib1 article-title: Emerging flaviviruses: the spread and resurgence of Japanese encephalitis, West Nile and dengue viruses publication-title: Nat. Med. doi: 10.1038/nm1144 – volume: 62 start-page: 867 year: 1965 ident: 10.1016/j.bbrc.2023.04.003_bib11 article-title: Hematodepressive virus diseases of Thailand publication-title: Ann. Intern. Med. doi: 10.7326/0003-4819-62-5-867 – volume: 33 start-page: 7051 year: 2015 ident: 10.1016/j.bbrc.2023.04.003_bib23 article-title: Mouse models of dengue virus infection for vaccine testing publication-title: Vaccine doi: 10.1016/j.vaccine.2015.09.112 – volume: 11 start-page: 532 year: 2011 ident: 10.1016/j.bbrc.2023.04.003_bib18 article-title: Immunity to dengue virus: a tale of original antigenic sin and tropical cytokine storms publication-title: Nat. Rev. Immunol. doi: 10.1038/nri3014 – volume: 12 year: 2021 ident: 10.1016/j.bbrc.2023.04.003_bib29 article-title: Innate immune cytokine profiling and biomarker identification for outcome in dengue patients publication-title: Front. Immunol. doi: 10.3389/fimmu.2021.677874 – volume: 26 start-page: 975 year: 1977 ident: 10.1016/j.bbrc.2023.04.003_bib21 article-title: Hemostatic and platelet kinetic studies in dengue hemorrhagic fever publication-title: Am. J. Trop. Med. Hyg. doi: 10.4269/ajtmh.1977.26.975 – volume: 385 start-page: 453 year: 2015 ident: 10.1016/j.bbrc.2023.04.003_bib19 article-title: Dengue publication-title: Lancet doi: 10.1016/S0140-6736(14)60572-9 – volume: 81 start-page: 6 year: 2009 ident: 10.1016/j.bbrc.2023.04.003_bib2 article-title: Molecular targets for flavivirus drug discovery publication-title: Antivir. Res. doi: 10.1016/j.antiviral.2008.08.004 – volume: 252 start-page: 68 year: 1966 ident: 10.1016/j.bbrc.2023.04.003_bib13 article-title: Hematologic phagocytosis in postmortem bone marrows of dengue hemorrhagic fever. (Hematologic phagocytosis in Thai hemorrhagic fever) publication-title: Am. J. Med. Sci. doi: 10.1097/00000441-196607000-00010 – volume: 89 start-page: 5847 year: 2015 ident: 10.1016/j.bbrc.2023.04.003_bib25 article-title: Dengue virus infection with highly neutralizing levels of cross-reactive antibodies causes acute lethal small intestinal pathology without a high level of viremia in mice publication-title: J. Virol. doi: 10.1128/JVI.00216-15
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Snippet	Previously, we demonstrated the utility of a recombinant chimeric flavivirus (DV2ChimV), which carries the premembrane (prM) and envelope (E) genes of a type 2...
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SubjectTerms	Animals Antibodies, Viral bone marrow Bone Marrow - pathology Bone marrow suppression Dengue Dengue Virus Flavivirus Hemorrhagic fever Humans intestines Japanese encephalitis virus liver megakaryocytes Mice Mice, Knockout Mouse model Vascular leakage viruses
Title	Chimeric flavivirus causes vascular leakage and bone marrow suppression in a mouse model
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