Timing of Blood Sample Processing Affects the Transcriptomic and Epigenomic Profiles in CD4+ T-cells of Atopic Subjects

• Published in: Cells (Basel, Switzerland) Vol. 11; no. 19; p. 2958
• Main Authors: Alhamdan, Fahd, Laubhahn, Kristina, Happle, Christine, Habener, Anika, Jirmo, Adan C., Thölken, Clemens, Conca, Raffaele, Chung, Ho-Ryun, Hansen, Gesine, Potaczek, Daniel P., Schaub, Bianca, Grychtol, Ruth, Garn, Holger
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 22.09.2022; MDPI
• Subjects: Acetylation; Atopy; Biobanks; Blood; CD4 antigen; CD4+ T-cells; ChIP sequencing; Chromatin; Cohort analysis; Epigenetic inheritance; Epigenetics; Gene expression; Genetic aspects; Genomes; Hay fever; Health aspects; histone modifications; Histones; Immunoprecipitation; Lymphocytes T; Medical examination; Medical research; Medicine, Experimental; Pediatrics; Peripheral blood; Rhinitis; RNA; T cells; Transcriptomics; Germany; United States > US

Optimal pre-analytical conditions for blood sample processing and isolation of selected cell populations for subsequent transcriptomic and epigenomic studies are required to obtain robust and reproducible results. This pilot study was conducted to investigate the potential effects of timing of CD4+...