Integrative analyses of metabolome and genome‐wide transcriptome reveal the regulatory network governing flavor formation in kiwifruit (Actinidia chinensis)

• Published in: The New phytologist Vol. 233; no. 1; pp. 373 - 389
• Main Authors: Wang, Ruochen, Shu, Peng, Zhang, Chi, Zhang, Junlin, Chen, Ya, Zhang, Yaoxin, Du, Kui, Xie, Yue, Li, Mingzhang, Ma, Tao, Zhang, Yang, Li, Zhengguo, Grierson, Don, Pirrello, Julien, Chen, Kunsong, Bouzayen, Mondher, Zhang, Bo, Liu, Mingchun
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.01.2022; Wiley
• Subjects: Actinidia; Actinidia - genetics; Actinidia - metabolism; Actinidia chinensis; Biochemistry, Molecular Biology; biosynthesis; data collection; Datasets; flavor; Flavors; Flavour; Fruit - genetics; Fruit - metabolism; fruit flavor; fruiting; Fruits; Gene Expression Regulation, Plant; Genes; Genomes; Genomics; Kiwifruit; Life Sciences; Metabolism; Metabolites; Metabolome; Organic acids; Plant Proteins - metabolism; Regulatory mechanisms (biology); regulatory network; Ripening; Sugar; Transcription; Transcription factors; transcriptome; Transcriptome - genetics; Transcriptomes; Vegetal Biology; Volatiles; weighted correlation network analysis (WGCNA); regulatory network; fruit flavor; weighted correlation network analysis (WGCNA); transcriptome; metabolome; kiwifruit; fruit flavor
• DOI: 10.1111/nph.17618
• ISSN: 0028-646X; 1469-8137

Summary Soluble sugars, organic acids and volatiles are important components that determine unique fruit flavor and consumer preferences. However, the metabolic dynamics and underlying regulatory networks that modulate overall flavor formation during fruit development and ripening remain largely unknown for most fruit species. In this study, by integrating flavor‐associated metabolism and transcriptome data from 12 fruit developmental and ripening stages of Actinidia chinensis cv Hongyang, we generated a global map of changes in the flavor‐related metabolites throughout development and ripening of kiwifruit. Using this dataset, we constructed complex regulatory networks allowing to identify key structural genes and transcription factors that regulate the metabolism of soluble sugars, organic acids and important volatiles in kiwifruit. Moreover, our study revealed the regulatory mechanism involving key transcription factors regulating flavor metabolism. The modulation of flavor metabolism by the identified key transcription factors was confirmed in different kiwifruit species providing the proof of concept that our dataset provides a suitable tool for clarification of the regulatory factors controlling flavor biosynthetic pathways that have not been previously illuminated. Overall, in addition to providing new insight into the metabolic regulation of flavor during fruit development and ripening, the outcome of our study establishes a foundation for flavor improvement in kiwifruit. See also the Commentary on this article by Fernie & Alseekh, 233: 8–10.