Insects and incest: Sib‐mating tolerance in natural populations of a parasitoid wasp

• Published in: Molecular ecology Vol. 29; no. 3; pp. 596 - 609
• Main Authors: Collet, Marie, Amat, Isabelle, Sauzet, Sandrine, Auguste, Alexandra, Fauvergue, Xavier, Mouton, Laurence, Desouhant, Emmanuel
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.02.2020; Wiley
• Subjects: Agricultural sciences; Agronomy; Animal biology; Animals; Avoidance; Avoidance behavior; Bioassays; Biochemistry, Molecular Biology; Biodiversity; Biodiversity and Ecology; Biological Evolution; Breeding; Cognitive science; Cognitive Sciences; Discrimination; Dispersal; Dispersion; Eggs; Environmental Sciences; Female; Females; Genomics; Genotype; Genotypes; genotyping; Global Changes; Hymenoptera; Inbreeding; Inbreeding - methods; Inbreeding depression; inbreeding tolerance; Incest; Insecta - genetics; Insecta - physiology; Insects; Invertebrate Zoology; kin recognition; Laboratories; Life Sciences; Male; Mate selection; Mating; mating behavior; Mating Preference, Animal - physiology; microsatellite repeats; Microsatellite Repeats - genetics; Microsatellites; Natural populations; Neurons and Cognition; Neuroscience; parasitic wasp; parasitic wasps; Parasitoids; Populations; Populations and Evolution; probability; Reproduction - physiology; Reproductive Biology; Sexual Behavior, Animal - physiology; Sexual reproduction; sl‐CSD; Superparasitism; Venturia canescens; Vertebrate Zoology; Wasps - genetics; Wasps - physiology; microsatellites; kin recognition; sl-CSD; Hymenoptera; inbreeding tolerance; parasitic wasp
• DOI: 10.1111/mec.15340
• ISSN: 0962-1083; 1365-294X

Sib‐mating avoidance is a pervasive behaviour that is expected to evolve in species subject to inbreeding depression. Although laboratory studies provide elegant demonstrations, small‐scaled bioassays minimize the costs of mate finding and choice, and thus may produce spurious findings. We therefore combined laboratory experiments with field observations to examine the existence of inbreeding avoidance using the parasitoid wasp Venturia canescens. In the laboratory, our approach consisted of mate‐choice experiments to assess kin discrimination in population cages with competitive interactions. A higher mating probability after sib rejections suggested that females could discriminate their sibs; however, in contrast to previous findings, sib‐mating avoidance was not observed. To compare our laboratory results to field data, we captured 241 individuals from two populations. Females laid eggs in the lab, and 226 daughters were obtained. All individuals were genotyped at 18 microsatellite loci, which allowed inference of the genotype of each female's mate and subsequently the relatedness within each mating pair. We found that the observed rate of sib‐mating did not differ from the probability that sibs encountered one another at random in the field, which is consistent with an absence of sib‐mating avoidance. In addition, we detected a weak but significant male‐biased dispersal, which could reduce encounters between sibs. We also found weak fitness costs associated with sib‐mating. As such, the sex‐biased dispersal that we found is probably sufficient to mitigate these costs. These results imply that kin discrimination has probably evolved for purposes other than mate choice, such as superparasitism avoidance.