Intestinal immune response of volunteers ingesting a strain of enteroadherent (HEp-2 cell-adherent) Escherichia coli
Services CVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue CVI About CVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM...
Saved in:
| Published in | Clinical and Diagnostic Laboratory Immunology Vol. 2; no. 1; pp. 10 - 13 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
American Society for Microbiology
01.01.1995
|
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Services
CVI
Citing Articles
Google Scholar
PubMed
Related Content
Social Bookmarking
CiteULike
Delicious
Digg
Facebook
Google+
Mendeley
Reddit
StumbleUpon
Twitter
current issue
CVI
About
CVI
Subscribers
Authors
Reviewers
Advertisers
Inquiries from the Press
Permissions & Commercial Reprints
ASM Journals Public Access Policy
CVI
RSS Feeds
1752 N Street N.W. • Washington DC 20036
202.737.3600 • 202.942.9355 fax • journals@asmusa.org
Print ISSN:
1556-6811
Online ISSN:
1556-679X
Copyright © 2014
by the
American Society for Microbiology.
For an alternate route to
CVI
.asm.org, visit:
CVI
|
|---|---|
| AbstractList | Enteroadherent Escherichia coli (EAEC) strains identified by adherence to HEp-2 tissue culture cells have been incriminated epidemiologically as important etiologic agents of diarrheal disease in both adult travelers and children in developing countries. One strain, JM 221, with no recognized E. coli virulence characteristics other than adherence to HEp-2 cells, caused diarrhea in 5 of 16 volunteers ingesting it. We studied the secretory immunoglobulin A (sIgA) responses to EAEC JM 221 of five volunteers with diarrhea and five volunteers who remained healthy after challenge. sIgA was extracted from stools obtained prechallenge and 7 days postchallenge. Total sIgA was standardized for all specimens. Specific sIgA titers were determined by dot blotting with the following JM 221 antigens: water-extractable surface antigens, whole cells, lipopolysaccharides, and outer membrane proteins. All five subjects who became ill had fourfold or greater rises in titers against each of the four antigens. The five subjects who remained healthy following challenge did not exhibit significant rises in titers to any JM 221 antigens, but their mean titers were significantly higher than the mean prechallenge titers of the volunteers with diarrhea, suggesting that high intestinal sIgA titers may be protective. The significant increases in intestinal antibody against JM 221 in the subjects who became ill is further evidence of the enteropathogenicity of EAEC strains. Enteroadherent Escherichia coli (EAEC) strains identified by adherence to HEp-2 tissue culture cells have been incriminated epidemiologically as important etiologic agents of diarrheal disease in both adult travelers and children in developing countries. One strain, JM 221, with no recognized E. coli virulence characteristics other than adherence to HEp-2 cells, caused diarrhea in 5 of 16 volunteers ingesting it. We studied the secretory immunoglobulin A (sIgA) responses to EAEC JM 221 of five volunteers with diarrhea and five volunteers who remained healthy after challenge. sIgA was extracted from stools obtained prechallenge and 7 days postchallenge. Total sIgA was standardized for all specimens. Specific sIgA titers were determined by dot blotting with the following JM 221 antigens: water-extractable surface antigens, whole cells, lipopolysaccharides, and outer membrane proteins. All five subjects who became ill had fourfold or greater rises in titers against each of the four antigens. The five subjects who remained healthy following challenge did not exhibit significant rises in titers to any JM 221 antigens, but their mean titers were significantly higher than the mean prechallenge titers of the volunteers with diarrhea, suggesting that high intestinal sIgA titers may be protective. The significant increases in intestinal antibody against JM 221 in the subjects who became ill is further evidence of the enteropathogenicity of EAEC strains.Enteroadherent Escherichia coli (EAEC) strains identified by adherence to HEp-2 tissue culture cells have been incriminated epidemiologically as important etiologic agents of diarrheal disease in both adult travelers and children in developing countries. One strain, JM 221, with no recognized E. coli virulence characteristics other than adherence to HEp-2 cells, caused diarrhea in 5 of 16 volunteers ingesting it. We studied the secretory immunoglobulin A (sIgA) responses to EAEC JM 221 of five volunteers with diarrhea and five volunteers who remained healthy after challenge. sIgA was extracted from stools obtained prechallenge and 7 days postchallenge. Total sIgA was standardized for all specimens. Specific sIgA titers were determined by dot blotting with the following JM 221 antigens: water-extractable surface antigens, whole cells, lipopolysaccharides, and outer membrane proteins. All five subjects who became ill had fourfold or greater rises in titers against each of the four antigens. The five subjects who remained healthy following challenge did not exhibit significant rises in titers to any JM 221 antigens, but their mean titers were significantly higher than the mean prechallenge titers of the volunteers with diarrhea, suggesting that high intestinal sIgA titers may be protective. The significant increases in intestinal antibody against JM 221 in the subjects who became ill is further evidence of the enteropathogenicity of EAEC strains. Services CVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue CVI About CVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy CVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 1556-6811 Online ISSN: 1556-679X Copyright © 2014 by the American Society for Microbiology. For an alternate route to CVI .asm.org, visit: CVI |
| Author | H F Gomez P C Johnson J J Mathewson H L DuPont |
| AuthorAffiliation | Center for Infectious Diseases, University of Texas Medical School, Houston |
| AuthorAffiliation_xml | – name: Center for Infectious Diseases, University of Texas Medical School, Houston |
| Author_xml | – sequence: 1 givenname: H F surname: Gomez fullname: Gomez, H F organization: Center for Infectious Diseases, University of Texas Medical School, Houston – sequence: 2 givenname: J J surname: Mathewson fullname: Mathewson, J J organization: Center for Infectious Diseases, University of Texas Medical School, Houston – sequence: 3 givenname: P C surname: Johnson fullname: Johnson, P C organization: Center for Infectious Diseases, University of Texas Medical School, Houston – sequence: 4 givenname: H L surname: DuPont fullname: DuPont, H L organization: Center for Infectious Diseases, University of Texas Medical School, Houston |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/7719900$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkcFu1DAQhi1UVNrCEyCETxUcEjxxEscHDmhZ6Eor9VIkbpbj2BujxF7spBVvj9PdVoVLTx7N_33WjOYcnTjvNEJvgeQARfNp9XW7yYscciAZ0Bw4r16gM6iqOqsZ_3nyUDcAr9B5jL8IKWndsFN0yliiCTlD08ZNOk7WyQHbcZydxkHHvXdRY2_wrR_mBOgQsXW7e3CHJY5TkNYtgE5p8LLrdUgl_nC13mcFVnoYsofmR7yOKpVW9VZi5Qf7Gr00coj6zfG9QD--rW9WV9n2-vtm9WWbqbIqp8yAbkAVlGtuOGsMq8EQ2RIgqmUVN6XsoDOUqq7oZG14WZa6bbuG8SQyVdEL9Pnw735uR92pNEyQg9gHO8rwR3hpxb-Js73Y-VsBjBBeJP_y6Af_e07bi9HGZTfptJ-jYKyAhlH2LAh1U1RlRRP47ulEj6Mc75FyeshV8DEGbR4JIGI5ulDdYEUh4L5BxXL0ZPH_LGUnOVm_bGWHZ9z3B7e3u_7OBi1kHJ-S9C9yQsE7 |
| CitedBy_id | crossref_primary_10_1128_CDLI_7_3_501_503_2000 crossref_primary_10_1128_CMR_00112_13 |
| ContentType | Journal Article |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM 7QL 7T5 C1K H94 7X8 5PM |
| DOI | 10.1128/CDLI.2.1.10-13.1995 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Bacteriology Abstracts (Microbiology B) Immunology Abstracts Environmental Sciences and Pollution Management AIDS and Cancer Research Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) AIDS and Cancer Research Abstracts Immunology Abstracts Bacteriology Abstracts (Microbiology B) Environmental Sciences and Pollution Management MEDLINE - Academic |
| DatabaseTitleList | CrossRef MEDLINE - Academic AIDS and Cancer Research Abstracts MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine Biology |
| EISSN | 1556-679X 1098-6588 |
| EndPage | 13 |
| ExternalDocumentID | PMC170092 7719900 10_1128_cdli_2_1_10_13_1995 cdli_2_1_10 |
| Genre | Journal Article Comparative Study |
| GroupedDBID | .GJ 18M 29B 2WC 53G 5GY 6J9 AAFWJ AAYXX ADBBV ALMA_UNASSIGNED_HOLDINGS BAWUL CITATION CS3 DIK EBS F5P GX1 HYE RHI RPM RSF TR2 W2D W8F WOQ CGR CUY CVF ECM EIF NPM RHF 7QL 7T5 C1K H94 7X8 5PM |
| ID | FETCH-LOGICAL-c454t-f1e81c239e9f978f761f0ab010cb759f4ad1df33cd2da6f9444ebbd8791e87c53 |
| ISSN | 1556-6811 1071-412X |
| IngestDate | Thu Aug 21 13:15:20 EDT 2025 Fri Jul 11 00:55:04 EDT 2025 Fri Jul 11 02:09:17 EDT 2025 Sat Sep 28 07:36:08 EDT 2024 Tue Jul 01 00:54:52 EDT 2025 Thu Apr 24 22:50:13 EDT 2025 Wed May 18 15:27:22 EDT 2016 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Language | English |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c454t-f1e81c239e9f978f761f0ab010cb759f4ad1df33cd2da6f9444ebbd8791e87c53 |
| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
| OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/170092 |
| PMID | 7719900 |
| PQID | 16825453 |
| PQPubID | 23462 |
| PageCount | 4 |
| ParticipantIDs | proquest_miscellaneous_16825453 proquest_miscellaneous_77218737 crossref_citationtrail_10_1128_cdli_2_1_10_13_1995 pubmedcentral_primary_oai_pubmedcentral_nih_gov_170092 pubmed_primary_7719900 crossref_primary_10_1128_cdli_2_1_10_13_1995 highwire_asm_cdli_2_1_10 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 1900 |
| PublicationDate | 19950101 1995-01-00 1995-Jan |
| PublicationDateYYYYMMDD | 1995-01-01 |
| PublicationDate_xml | – month: 01 year: 1995 text: 19950101 day: 01 |
| PublicationDecade | 1990 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | Clinical and Diagnostic Laboratory Immunology |
| PublicationTitleAlternate | Clin Diagn Lab Immunol |
| PublicationYear | 1995 |
| Publisher | American Society for Microbiology |
| Publisher_xml | – name: American Society for Microbiology |
| References | 4580564 - J Bacteriol. 1973 Sep;115(3):717-22 3525699 - J Infect Dis. 1986 Sep;154(3):524-7 2656874 - J Infect Dis. 1989 Jun;159(6):1057-60 3312288 - J Clin Microbiol. 1987 Oct;25(10):1917-9 3882849 - J Infect Dis. 1985 Mar;151(3):471-5 2899125 - J Infect Dis. 1988 Jul;158(1):70-9 3514359 - Gastroenterology. 1986 May;90(5 Pt 1):1217-22 6133088 - Lancet. 1983 May 7;1(8332):1048 3053924 - J Infect Dis. 1988 Nov;158(5):1108-12 3721580 - Infect Immun. 1986 Jul;53(1):57-63 2656875 - J Infect Dis. 1989 Jun;159(6):1061-4 1940478 - J Infect Dis. 1991 Nov;164(5):979-82 |
| References_xml | – reference: 3525699 - J Infect Dis. 1986 Sep;154(3):524-7 – reference: 2899125 - J Infect Dis. 1988 Jul;158(1):70-9 – reference: 3882849 - J Infect Dis. 1985 Mar;151(3):471-5 – reference: 4580564 - J Bacteriol. 1973 Sep;115(3):717-22 – reference: 3514359 - Gastroenterology. 1986 May;90(5 Pt 1):1217-22 – reference: 2656874 - J Infect Dis. 1989 Jun;159(6):1057-60 – reference: 3312288 - J Clin Microbiol. 1987 Oct;25(10):1917-9 – reference: 3053924 - J Infect Dis. 1988 Nov;158(5):1108-12 – reference: 2656875 - J Infect Dis. 1989 Jun;159(6):1061-4 – reference: 6133088 - Lancet. 1983 May 7;1(8332):1048 – reference: 3721580 - Infect Immun. 1986 Jul;53(1):57-63 – reference: 1940478 - J Infect Dis. 1991 Nov;164(5):979-82 |
| SSID | ssj0043687 ssj0014330 |
| Score | 1.4854707 |
| Snippet | Services
CVI
Citing Articles
Google Scholar
PubMed
Related Content
Social Bookmarking
CiteULike
Delicious
Digg
Facebook
Google+
Mendeley
Reddit
StumbleUpon... Enteroadherent Escherichia coli (EAEC) strains identified by adherence to HEp-2 tissue culture cells have been incriminated epidemiologically as important... |
| SourceID | pubmedcentral proquest pubmed crossref highwire |
| SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 10 |
| SubjectTerms | Administration, Oral Adult Antibodies, Bacterial - biosynthesis Antibodies, Bacterial - immunology Antibodies, Bacterial - isolation & purification Antigens, Bacterial - immunology Bacterial Adhesion Diarrhea - immunology Diarrhea - microbiology Escherichia coli Escherichia coli - immunology Escherichia coli - pathogenicity Escherichia coli - physiology Escherichia coli Infections - immunology Escherichia coli Infections - microbiology Feces - chemistry Humans Immunoglobulin A, Secretory - biosynthesis Immunoglobulin A, Secretory - immunology Immunoglobulin A, Secretory - isolation & purification Intestines - immunology Intestines - microbiology Tumor Cells, Cultured Virulence |
| Title | Intestinal immune response of volunteers ingesting a strain of enteroadherent (HEp-2 cell-adherent) Escherichia coli |
| URI | http://cvi.asm.org/content/2/1/10.abstract https://www.ncbi.nlm.nih.gov/pubmed/7719900 https://www.proquest.com/docview/16825453 https://www.proquest.com/docview/77218737 https://pubmed.ncbi.nlm.nih.gov/PMC170092 |
| Volume | 2 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bb9MwFLa6IRAvCAbTymX4gQfQSKkviZNHNArVUBFIm7S3yIkTLdLWTmv7AL-Mn8c5tnPbysToQ1Q5jmPnfD4-Pj4XQt4UcRwpmbFAJEUGG5RYBhpgEkS5MSrURoocHZxn36LpiTw6DU8Hg98dq6X1Khvlvzb6lfwPVaEM6IpesnegbNMoFMB_oC9cgcJw_ScaozoPpigKlBX6eWAKFGvyahUByHigAnroou5uaQ2cNXqH6MoKiRiO82qhzVlRBx6dTi4DfoDK_KAuRrXBZImkrdAs-gCAU3Ul2sPatRI18MYZ7mEQWI8uPMK3feup778sLpzmetpaFs-0TTzurACO2uMqn8PLirutTvfT-vvChUyYescJ4335wo7qwnFbkG8CyWw-dViMfFkSByAWxV0WzW8g0bFbbxHrFm7n03pzSeDo5pCb82rER2yExigCfTTDdgWsT_2vLYyNuaLdKPE4xUZSnrLUHgOm2MgWucdhgyJqPZE_v5JCuDgYfoA-3hU08mFDT_oyUR2netOe57rpbkcWOn5MHvlNDP3oEPmEDIr5Drnv0pr-3CEPZt5g4ylZtRClDqK0hihdlLSFKG0gSjV1EMUKfYjStxagtAfQd7QDT4rwfEZOPk-OD6eBT_QR5DKUq6BkRcxyDuwiKRMVlypi5VhnYzbOMxUmpdSGmVKI3HCjozKRUhZZZmKVwIMqD8Uu2Z4v5sUeoXHGRaG1yRXPpeZcgyQAv0wonjGT6SHh9adOcx8FH8d0nt5C5CF53zx06YLA3F59r6ZhqpcX3SpD8rqmaQp8HD-XnheL9TJlEepqQvH3GgAzFiuhhmTXYaDpi1Lw2jE0HvXA0dzHEPL9O_PqzIaSx-icCX9-t_G9IA_b2fySbK-u1sUrEM1X2T7Z-voj3rdz4Q9GUOie |
| linkProvider | National Library of Medicine |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Intestinal+immune+response+of+volunteers+ingesting+a+strain+of+enteroadherent+%28HEp-2+cell-adherent%29+Escherichia+coli&rft.jtitle=Clinical+and+diagnostic+laboratory+immunology&rft.au=Gomez%2C+H+F&rft.au=Mathewson%2C+J+J&rft.au=Johnson%2C+P+C&rft.au=DuPont%2C+H+L&rft.date=1995-01-01&rft.issn=1071-412X&rft.eissn=1098-6588&rft.volume=2&rft.issue=1&rft.spage=10&rft.epage=13&rft_id=info:doi/10.1128%2Fcdli.2.1.10-13.1995&rft.externalDBID=n%2Fa&rft.externalDocID=10_1128_cdli_2_1_10_13_1995 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1556-6811&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1556-6811&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1556-6811&client=summon |