Rapid detection of multidrug-resistant Mycobacterium tuberculosis by multiplex allele-specific polymerase chain reaction

• Published in: The international journal of tuberculosis and lung disease Vol. 15; no. 4; pp. 496 - 501
• Main Authors: IMPERIALE, B. R, CATALDI, A. A, MORCILLO, N. S
• Format: Journal Article
• Language: English
• Published: Paris, France IUATLD 01.04.2011; International Union against Tuberculosis and Lung Disease
• Subjects: Alleles; Antitubercular Agents - pharmacology; Argentina; Bacterial diseases; Biological and medical sciences; Drug Resistance, Multiple, Bacterial; Genes, Bacterial; Human bacterial diseases; Humans; Infectious diseases; Isoniazid - pharmacology; MAS-PCR; Medical sciences; Microbial Sensitivity Tests; Multidrug-Resistant; Mutation; Mycobacterium tuberculosis; Mycobacterium tuberculosis - drug effects; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - isolation & purification; Pneumology; Polymerase Chain Reaction - methods; Respiratory system : syndromes and miscellaneous diseases; Rifampin - pharmacology; Tuberculosis; Tuberculosis and atypical mycobacterial infections; Argentina; MAS-PCR; Respiratory disease; Mycobacterial infection; multidrug-resistant; Infection; Mass; Polymerase chain reaction; Allele; Rapid technique; Tuberculosis; Mycobacterium tuberculosis; Mycobacteriales; Multiple resistance; Bacteriosis; Mycobacteriaceae; Bacteria; Tumor; Actinomycetes; Molecular biology; Pneumology

SETTING: Dr Cetrangolo Hospital, Buenos Aires Province, Argentina.OBJECTIVE: To evaluate a multiplex allele-specific polymerase chain reaction (MAS-PCR) to detect multidrug-resistant tuberculosis (MDR-TB) clinical isolates and to describe the main mutations conferring resistance to isoniazid (INH) a...