Tolerance of S. cerevisiae and Z. mobilis to inhibitors produced during dilute acid hydrolysis of soybean meal

• Published in: Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes Vol. 49; no. 4; pp. 305 - 311
• Main Authors: Lujan-Rhenals, Deivis E., Morawicki, Rubén O., Ricke, Steven C.
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Taylor & Francis Group 01.01.2014; Taylor & Francis; Taylor & Francis Ltd
• Subjects: Acetic acid; Acetic Acid - analysis; Acetic Acid - pharmacology; acid hydrolysis; Acids - chemistry; Activated carbon; Agronomy. Soil science and plant productions; Animal, plant and microbial ecology; Applied ecology; Applied sciences; bioethanol; Biofuel production; Biological and medical sciences; Biomass; Biotechnology; Chemical compounds; Energy; Ethanol - metabolism; Exact sciences and technology; Fermentation; Fundamental and applied biological sciences. Psychology; Furaldehyde - analogs & derivatives; Furaldehyde - analysis; Furaldehyde - pharmacology; furfural; General agronomy. Plant production; Glycine max - chemistry; Glycine max - metabolism; Hydrolysis; hydroxymethyl furfural; Industrial applications and implications. Economical aspects; Microorganisms; Natural energy; Saccharomyces cerevisiae; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; soybean meal; Soybeans; Sulfuric acid; Use of agricultural and forest wastes. Biomass use, bioconversion; Yeast; Zymomonas - drug effects; Zymomonas - growth & development; Zymomonas - metabolism; Zymomonas mobilis; Ascomycota; Furfural; Ethanol; Acidolysis; Tolerance; Biofuel; hydroxymethyl furfural; Fungi; Acid digestion; Bacteria; Environment; Inhibitor; bioethanol; Acetic acid; acid hydrolysis; Saccharomyces cerevisiae; Zymomonas mobilis; Soybean meal
• ISSN: 0360-1234; 1532-4109
• DOI: 10.1080/03601234.2014.868683

The objective of this research was to determine the minimum inhibitory concentration of 5-hydroxymethyl furfural, furfural, and acetic acid on Saccharomyces cerevisiae (NRRL Y-2233) and Zymomonas mobilis subspecies mobilis (NRRL B-4286) in both detoxified hydrolyzed soybean meal and synthetic YM broth spiked with the three compounds. Soybean meal was hydrolyzed with dilute sulfuric acid (0.0, 0.5, 1.25, and 2.0% wt v −1 ) at three temperatures (105, 120, and 135°C) and three durations (15, 30, and 45 min) followed by detoxification with activated carbon. Of all the combinations, only the treatments obtained at 135°C, 2.0% H 2 SO 4 , and 45 min and the one at 135°C, 1.25% H 2 SO 4 , and 45 min showed inhibition in the growth of the tested microorganisms. Spiked YM broths showed inhibition for the highest levels of inhibitors, either applied individually or in combination.