Isolation of the active constituents in natural materials by ‘heart-cutting’ isocratic reversed-phase two-dimensional liquid chromatography

• Published in: Journal of Chromatography A Vol. 1036; no. 1; pp. 15 - 24
• Main Authors: Wong, Victor, Shalliker, R.A.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 14.05.2004; Elsevier
• Subjects: Analytical chemistry; Biological and medical sciences; Biological Factors - chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - methods; Clerodendrum floribundum; Exact sciences and technology; General pharmacology; Heart-cutting; Liquid chromatography, two-dimensional; Medical sciences; Multidimensional techniques; Other chromatographic methods; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Preparative chromatography; Multidimensional techniques; Clerodendrum floribundum; Liquid chromatography, two-dimensional; Heart-cutting; Preparative chromatography; Purification; Two dimensional chromatography; Pharmacognosy; Heart-cutting; Multidimensional techniques; Liquid chromatography; Liquid chromatography; Isocratic condition; Medicinal plant; Reversed phase chromatography; Verbenaceae; Dicotyledones; Angiospermae; Plant origin; Isolation; Active ingredient; two-dimensional; Preparative chromatography; Clerodendrum floribundum; Spermatophyta

A multidimensional heart-cutting reversed-phase HPLC separation approach, where two columns were operated independently via two six-port two-position switching valves, was employed in the isolation of a major bioactive found in the ethanol–water (80:20) crude extract of Clerodendrum floribundum. In...