Isolation of the active constituents in natural materials by ‘heart-cutting’ isocratic reversed-phase two-dimensional liquid chromatography

• Published in: Journal of Chromatography A Vol. 1036; no. 1; pp. 15 - 24
• Main Authors: Wong, Victor, Shalliker, R.A.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 14.05.2004; Elsevier
• Subjects: Analytical chemistry; Biological and medical sciences; Biological Factors - chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - methods; Clerodendrum floribundum; Exact sciences and technology; General pharmacology; Heart-cutting; Liquid chromatography, two-dimensional; Medical sciences; Multidimensional techniques; Other chromatographic methods; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Preparative chromatography; Multidimensional techniques; Clerodendrum floribundum; Liquid chromatography, two-dimensional; Heart-cutting; Preparative chromatography; Purification; Two dimensional chromatography; Pharmacognosy; Heart-cutting; Multidimensional techniques; Liquid chromatography; Liquid chromatography; Isocratic condition; Medicinal plant; Reversed phase chromatography; Verbenaceae; Dicotyledones; Angiospermae; Plant origin; Isolation; Active ingredient; two-dimensional; Preparative chromatography; Clerodendrum floribundum; Spermatophyta
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2004.02.003

A multidimensional heart-cutting reversed-phase HPLC separation approach, where two columns were operated independently via two six-port two-position switching valves, was employed in the isolation of a major bioactive found in the ethanol–water (80:20) crude extract of Clerodendrum floribundum. In this mode of operation, the specific productivity of the multidimensional approach under overload conditions was twice that of conventional gradient methods with the same loading factor. Isolated sample purities were greater than 99% with recoveries of 95%. The independent operation of each of the two columns employed in the multidimensional approach allowed the cycle time to be less than 7 min, compared to 23 min in the gradient elution single-dimension mode of operation.