Pyruvate Kinase Triggers a Metabolic Feedback Loop that Controls Redox Metabolism in Respiring Cells

• Published in: Cell metabolism Vol. 14; no. 3; pp. 415 - 427
• Main Authors: Grüning, Nana-Maria, Rinnerthaler, Mark, Bluemlein, Katharina, Mülleder, Michael, Wamelink, Mirjam M.C., Lehrach, Hans, Jakobs, Cornelis, Breitenbach, Michael, Ralser, Markus
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 07.09.2011; Cell Press
• Subjects: Cell Proliferation; Cell Respiration - physiology; Chromatography, Liquid; Feedback, Physiological; Galactose - metabolism; Gene Expression Regulation, Fungal; Glucose - metabolism; Glycolysis - physiology; Oxidation-Reduction; Oxidative Stress - genetics; Pentose Phosphate Pathway; Phosphoenolpyruvate - metabolism; Polymerase Chain Reaction; Pyruvate Kinase - genetics; Pyruvate Kinase - metabolism; Reactive Oxygen Species - metabolism; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Tandem Mass Spectrometry; Triose-Phosphate Isomerase - genetics; Triose-Phosphate Isomerase - metabolism

In proliferating cells, a transition from aerobic to anaerobic metabolism is known as the Warburg effect, whose reversal inhibits cancer cell proliferation. Studying its regulator pyruvate kinase (PYK) in yeast, we discovered that central metabolism is self-adapting to synchronize redox metabolism w...