Architecture of African swine fever virus and implications for viral assembly

• Published in: Science (American Association for the Advancement of Science) Vol. 366; no. 6465; pp. 640 - 644
• Main Authors: Wang, Nan, Zhao, Dongming, Wang, Jialing, Zhang, Yangling, Wang, Ming, Gao, Yan, Li, Fang, Wang, Jingfei, Bu, Zhigao, Rao, Zihe, Wang, Xiangxi
• Format: Journal Article
• Language: English
• Published: United States American Association for the Advancement of Science 01.11.2019; The American Association for the Advancement of Science
• Subjects: African swine fever; African Swine Fever Virus - chemistry; African Swine Fever Virus - physiology; African Swine Fever Virus - ultrastructure; Animals; Asfarviridae; Capsid - physiology; Capsid - ultrastructure; Capsid Proteins - chemistry; Capsid Proteins - immunology; Capsid Proteins - ultrastructure; Cells, Cultured; Cryoelectron Microscopy; Deoxyribonucleic acid; DNA; Epitopes; Models, Molecular; Protein Conformation; Protein Domains; Protein Folding; Protein Structure, Secondary; Proteins; Swine; Vaccine development; Vaccines; Virion - chemistry; Virion - ultrastructure; Virus Assembly; Viruses

African swine fever virus (ASFV) is a giant and complex DNA virus that causes a highly contagious and often lethal swine disease for which no vaccine is available. Using an optimized image reconstruction strategy, we solved the ASFV capsid structure up to 4.1 angstroms, which is built from 17,280 pr...