Engineering death resistance in CHO cells for improved perfusion culture

• Published in: mAbs Vol. 14; no. 1; p. 2083465
• Main Authors: MacDonald, Michael A., Nöbel, Matthias, Martínez, Verónica S., Baker, Kym, Shave, Evan, Gray, Peter P., Mahler, Stephen, Munro, Trent, Nielsen, Lars K., Marcellin, Esteban
• Format: Journal Article
• Language: English
• Published: Taylor & Francis 31.12.2022; Taylor & Francis Group
• Subjects: bioprocessing; cell line development; genetic engineering; mAb glycosylation; mAb manufacturing; perfusion

The reliable and cost-efficient manufacturing of monoclonal antibodies (mAbs) is essential to fulfil their ever-growing demand. Cell death in bioreactors reduces productivity and product quality, and is largely attributed to apoptosis. In perfusion bioreactors, this leads to the necessity of a bleed...