STRIDE—a fluorescence method for direct, specific in situ detection of individual single- or double-strand DNA breaks in fixed cells

• Published in: Nucleic acids research Vol. 48; no. 3; p. e14
• Main Authors: Kordon, Magdalena M, Zarębski, Mirosław, Solarczyk, Kamil, Ma, Hanhui, Pederson, Thoru, Dobrucki, Jurek W
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 20.02.2020
• Subjects: Cell Line; Cells, Cultured; CRISPR-Associated Protein 9; DNA Breaks, Double-Stranded; DNA Breaks, Single-Stranded; Fluorescent Dyes; HeLa Cells; Humans; Methods Online; Microscopy, Fluorescence; Nucleic Acid Hybridization; Oligonucleotide Probes; Tissue Fixation

Abstract We here describe a technique termed STRIDE (SensiTive Recognition of Individual DNA Ends), which enables highly sensitive, specific, direct in situ detection of single- or double-strand DNA breaks (sSTRIDE or dSTRIDE), in nuclei of single cells, using fluorescence microscopy. The sensitivit...