Detection of tyrosine and monitoring tyrosinase activity using an enzyme cascade-triggered colorimetric reaction
The aromatic amino acid tyrosine is an essential precursor for the synthesis of catecholamines, including l -DOPA, tyramine, and dopamine. A number of metabolic disorders have been linked to abnormal tyrosine levels in biological fluids. In this study, we developed an enzyme cascade-triggered colori...
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| Published in | RSC advances Vol. 1; no. 5; pp. 29745 - 2975 |
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| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
England
Royal Society of Chemistry
12.08.2020
The Royal Society of Chemistry |
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| Abstract | The aromatic amino acid tyrosine is an essential precursor for the synthesis of catecholamines, including
l
-DOPA, tyramine, and dopamine. A number of metabolic disorders have been linked to abnormal tyrosine levels in biological fluids. In this study, we developed an enzyme cascade-triggered colorimetric reaction for the detection of tyrosine, based on the formation of yellow pigment (betalamic acid) and red fluorometric betaxanthin. Tyrosinase converts tyrosine to
l
-DOPA, and DOPA-dioxygenase catalyzes oxidative cleavage of
l
-DOPA into betalamic acid. Response is linear for tyrosine from 5 to 100 M, and the detection limit (LOD) is 2.74 μM. The enzyme cascade reaction was applied to monitor tyrosinase activity and tyrosinase inhibition assays. Lastly, the performance of the proposed biosensor proved successful in the analysis of urine samples without the need for pre-treatment.
We developed an enzyme cascade-triggered colorimetric reaction for the detection of tyrosine, based on the formation of yellow pigment (betalamic acid) and red fluorometric betaxanthin. |
|---|---|
| AbstractList | The aromatic amino acid tyrosine is an essential precursor for the synthesis of catecholamines, including l-DOPA, tyramine, and dopamine. A number of metabolic disorders have been linked to abnormal tyrosine levels in biological fluids. In this study, we developed an enzyme cascade-triggered colorimetric reaction for the detection of tyrosine, based on the formation of yellow pigment (betalamic acid) and red fluorometric betaxanthin. Tyrosinase converts tyrosine to l-DOPA, and DOPA-dioxygenase catalyzes oxidative cleavage of l-DOPA into betalamic acid. Response is linear for tyrosine from 5 to 100 μM, and the detection limit (LOD) is 2.74 μM. The enzyme cascade reaction was applied to monitor tyrosinase activity and tyrosinase inhibition assays. Lastly, the performance of the proposed biosensor proved successful in the analysis of urine samples without the need for pre-treatment. The aromatic amino acid tyrosine is an essential precursor for the synthesis of catecholamines, including l -DOPA, tyramine, and dopamine. A number of metabolic disorders have been linked to abnormal tyrosine levels in biological fluids. In this study, we developed an enzyme cascade-triggered colorimetric reaction for the detection of tyrosine, based on the formation of yellow pigment (betalamic acid) and red fluorometric betaxanthin. Tyrosinase converts tyrosine to l -DOPA, and DOPA-dioxygenase catalyzes oxidative cleavage of l -DOPA into betalamic acid. Response is linear for tyrosine from 5 to 100 M, and the detection limit (LOD) is 2.74 μM. The enzyme cascade reaction was applied to monitor tyrosinase activity and tyrosinase inhibition assays. Lastly, the performance of the proposed biosensor proved successful in the analysis of urine samples without the need for pre-treatment. We developed an enzyme cascade-triggered colorimetric reaction for the detection of tyrosine, based on the formation of yellow pigment (betalamic acid) and red fluorometric betaxanthin. The aromatic amino acid tyrosine is an essential precursor for the synthesis of catecholamines, including l -DOPA, tyramine, and dopamine. A number of metabolic disorders have been linked to abnormal tyrosine levels in biological fluids. In this study, we developed an enzyme cascade-triggered colorimetric reaction for the detection of tyrosine, based on the formation of yellow pigment (betalamic acid) and red fluorometric betaxanthin. Tyrosinase converts tyrosine to l -DOPA, and DOPA-dioxygenase catalyzes oxidative cleavage of l -DOPA into betalamic acid. Response is linear for tyrosine from 5 to 100 μM, and the detection limit (LOD) is 2.74 μM. The enzyme cascade reaction was applied to monitor tyrosinase activity and tyrosinase inhibition assays. Lastly, the performance of the proposed biosensor proved successful in the analysis of urine samples without the need for pre-treatment. The aromatic amino acid tyrosine is an essential precursor for the synthesis of catecholamines, including l -DOPA, tyramine, and dopamine. A number of metabolic disorders have been linked to abnormal tyrosine levels in biological fluids. In this study, we developed an enzyme cascade-triggered colorimetric reaction for the detection of tyrosine, based on the formation of yellow pigment (betalamic acid) and red fluorometric betaxanthin. Tyrosinase converts tyrosine to l -DOPA, and DOPA-dioxygenase catalyzes oxidative cleavage of l -DOPA into betalamic acid. Response is linear for tyrosine from 5 to 100 μM, and the detection limit (LOD) is 2.74 μM. The enzyme cascade reaction was applied to monitor tyrosinase activity and tyrosinase inhibition assays. Lastly, the performance of the proposed biosensor proved successful in the analysis of urine samples without the need for pre-treatment. We developed an enzyme cascade-triggered colorimetric reaction for the detection of tyrosine, based on the formation of yellow pigment (betalamic acid) and red fluorometric betaxanthin. |
| Author | Chen, Huei-Yu Yeh, Yi-Chun |
| AuthorAffiliation | Department of Chemistry National Taiwan Normal University |
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l
-DOPA, tyramine, and dopamine. A number of... The aromatic amino acid tyrosine is an essential precursor for the synthesis of catecholamines, including l-DOPA, tyramine, and dopamine. A number of metabolic... |
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| SubjectTerms | Biosensors Cascade chemical reactions Chemistry Colorimetry Dihydroxyphenylalanine Dopamine Enzymes Metabolic disorders Pretreatment Tyrosinase Tyrosine |
| Title | Detection of tyrosine and monitoring tyrosinase activity using an enzyme cascade-triggered colorimetric reaction |
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