Proteomic changes in rat gastrocnemius muscle after botulinum toxin a injection
To observe the changes in protein expression induced by botulinum toxin A (BoNT-A) injection and to characterize the molecular and cellular action of mechanisms of BoNT-A injection on skeletal muscles using proteomic elements as biomarkers. BoNT-A was injected into left gastrocnemius muscles of 12 S...
Saved in:
Published in | Annals of rehabilitation medicine Vol. 37; no. 2; pp. 157 - 166 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Korea (South)
Korean Academy of Rehabilitation Medicine
01.04.2013
대한재활의학회 |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | To observe the changes in protein expression induced by botulinum toxin A (BoNT-A) injection and to characterize the molecular and cellular action of mechanisms of BoNT-A injection on skeletal muscles using proteomic elements as biomarkers.
BoNT-A was injected into left gastrocnemius muscles of 12 Sprague-Dawley rats (2 months of age) at a dosage of 5 units/kg body weight. For the controls same volume of normal saline was injected to right gastrocnemius muscle of each rat. Muscle samples were obtained at 4 time points (3 rats per time point): 3, 7, 14, and 56 day post-injection. To reveal the alterations in muscle protein, we performed 2-dimensional electrophoresis (2DE) and compared Botox group and normal saline group at each time point. Altered protein spots in 2DE were identified using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometer (MALDI-TOF MS) proteomics analysis.
Compared with normal saline group, 46 protein spots showed changed protein expression. Twelve protein spots demonstrated increased volume and 34 protein spots demonstrated decreased volume. Among spots of decreased volume, 17 spots showed statistically significant differences. Thirty-eight identified proteins were associated with alterations in energy metabolism, muscle contractile function, transcription, translation, cell proliferation, and cellular stress response.
BoNT-A gives influences on muscle contractile function and energy metabolism directly or indirectly besides neurotoxic effects. Proteomic expression provides better understanding about the effect of BoNT-A on skeletal muscle. |
---|---|
AbstractList | To observe the changes in protein expression induced by botulinum toxin A (BoNT-A) injection and to characterize the molecular and cellular action of mechanisms of BoNT-A injection on skeletal muscles using proteomic elements as biomarkers.
BoNT-A was injected into left gastrocnemius muscles of 12 Sprague-Dawley rats (2 months of age) at a dosage of 5 units/kg body weight. For the controls same volume of normal saline was injected to right gastrocnemius muscle of each rat. Muscle samples were obtained at 4 time points (3 rats per time point): 3, 7, 14, and 56 day post-injection. To reveal the alterations in muscle protein, we performed 2-dimensional electrophoresis (2DE) and compared Botox group and normal saline group at each time point. Altered protein spots in 2DE were identified using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometer (MALDI-TOF MS) proteomics analysis.
Compared with normal saline group, 46 protein spots showed changed protein expression. Twelve protein spots demonstrated increased volume and 34 protein spots demonstrated decreased volume. Among spots of decreased volume, 17 spots showed statistically significant differences. Thirty-eight identified proteins were associated with alterations in energy metabolism, muscle contractile function, transcription, translation, cell proliferation, and cellular stress response.
BoNT-A gives influences on muscle contractile function and energy metabolism directly or indirectly besides neurotoxic effects. Proteomic expression provides better understanding about the effect of BoNT-A on skeletal muscle. Objective To observe the changes in protein expression induced by botulinum toxin A (BoNT-A) injection and to characterize the molecular and cellular action of mechanisms of BoNT-A injection on skeletal muscles using proteomic elements as biomarkers.Methods BoNT-A was injected into left gastrocnemius muscles of 12 Sprague-Dawley rats (2 months of age) at a dosage of 5 units/kg body weight. For the controls same volume of normal saline was injected to right gastrocnemius muscle of each rat. Muscle samples were obtained at 4 time points (3 rats per time point): 3, 7, 14, and 56 day post-injection. To reveal the alterations in muscle protein, we performed 2-dimensional electrophoresis (2DE) and compared Botox group and normal saline group at each time point. Altered protein spots in 2DE were identified using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometer (MALDI-TOF MS) proteomics analysis.Results Compared with normal saline group, 46 protein spots showed changed protein expression. Twelve protein spots demonstrated increased volume and 34 protein spots demonstrated decreased volume. Among spots of decreased volume, 17 spots showed statistically significant differences. Thirty-eight identified proteins were associated with alterations in energy metabolism, muscle contractile function, transcription, translation, cell proliferation, and cellular stress response.Conclusion BoNT-A gives influences on muscle contractile function and energy metabolism directly or indirectly besides neurotoxic effects. Proteomic expression provides better understanding about the effect of BoNT-A on skeletal muscle. KCI Citation Count: 0 |
Author | Kim, Hyun Dong Kang, Mi Seon Han, Nami Eom, Mi-Ja You, Jun Myeong Han, Jin Kim, Hyoung Kyu |
AuthorAffiliation | 1 Department of Physical Medicine and Rehabilitation, Inje University Busan Paik Hospital, Inje University College of Medicine, Busan, Korea 3 Department of Pathology, Inje University Busan Paik Hospital, Inje University College of Medicine, Busan, Korea 2 Mitochondrial Signaling Laboratory, Department of Physiology and Biophysics, Inje University Busan Paik Hospital, Inje University College of Medicine, Busan, Korea |
AuthorAffiliation_xml | – name: 2 Mitochondrial Signaling Laboratory, Department of Physiology and Biophysics, Inje University Busan Paik Hospital, Inje University College of Medicine, Busan, Korea – name: 1 Department of Physical Medicine and Rehabilitation, Inje University Busan Paik Hospital, Inje University College of Medicine, Busan, Korea – name: 3 Department of Pathology, Inje University Busan Paik Hospital, Inje University College of Medicine, Busan, Korea |
Author_xml | – sequence: 1 givenname: Nami surname: Han fullname: Han, Nami organization: Department of Physical Medicine and Rehabilitation, Inje University Busan Paik Hospital, Inje University College of Medicine, Busan, Korea – sequence: 2 givenname: Hyun Dong surname: Kim fullname: Kim, Hyun Dong – sequence: 3 givenname: Mi-Ja surname: Eom fullname: Eom, Mi-Ja – sequence: 4 givenname: Jun Myeong surname: You fullname: You, Jun Myeong – sequence: 5 givenname: Jin surname: Han fullname: Han, Jin – sequence: 6 givenname: Hyoung Kyu surname: Kim fullname: Kim, Hyoung Kyu – sequence: 7 givenname: Mi Seon surname: Kang fullname: Kang, Mi Seon |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/23705109$$D View this record in MEDLINE/PubMed https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001763937$$DAccess content in National Research Foundation of Korea (NRF) |
BookMark | eNpVkctKBDEQRYMoPucD3Egv3Uybd9IbQcTHgKCIrkM6k4zR6USStOjfGx0dtDZVUPfcgrp7YDPEYAE4RLBljLATnYYWQ0RaIlrcIiY2wC7GhE4hZ2RzPVO2AyY5P8NajKMOo22wg4mADMFuF9zepVhsHLxpzJMOC5sbH5qkS7PQuaRogh38mJthzGZpG-2KTU0fy7j0YRyaEt-rXFfm2ZriYzgAW04vs5389H3weHnxcH49vbm9mp2f3UwNZbRMWeeo6KS0UBND5xh2gs-xRdQx56QkAkspjaSSu74zUmrBNTW451Az7WBP9sHxyjckp16MV1H7776I6iWps_uHmZKYU8Sr9HQlfR37wc6NDSXppXpNftDp4xv8vwn-qdq8KcI5pIJVA7QyMCnmnKxbswiqryxUzUJ9ZaGIUFjVLCpz9Pfomvj9PPkEVdKItA |
CitedBy_id | crossref_primary_10_1177_20587392211039942 crossref_primary_10_1097_01_EHX_0000446576_66077_bb crossref_primary_10_1152_ajpendo_00433_2019 crossref_primary_10_1186_s40064_016_2680_9 crossref_primary_10_1007_s00253_021_11640_0 crossref_primary_10_1007_s11033_014_3109_6 crossref_primary_10_1097_PRS_0000000000004981 crossref_primary_10_1038_s41598_017_14997_3 crossref_primary_10_1002_jor_22646 |
Cites_doi | 10.1016/0003-2697(76)90527-3 10.1073/pnas.96.6.3200 10.1046/j.1432-1327.1999.00486.x 10.1136/jnnp.58.2.232 10.1083/jcb.201004052 10.1016/S1570-0232(02)00056-9 10.1002/pmic.200700859 10.1016/0022-510X(71)90130-4 10.1016/0168-5597(94)90150-3 10.1111/j.1582-4934.2006.tb00404.x 10.1002/1615-9861(200205)2:5<543::AID-PROT543>3.0.CO;2-K 10.1096/fj.04-3084fje 10.1136/bmj.309.6968.1526 10.1002/1522-2683(20000601)21:11<2228::AID-ELPS2228>3.0.CO;2-V 10.1016/j.freeradbiomed.2005.02.026 10.1017/S0317167100038063 10.1044/1092-4388(2002/037) 10.1111/j.1742-1241.1989.tb08783.x 10.1002/1615-9861(200103)1:3<424::AID-PROT424>3.0.CO;2-Y 10.1016/S0161-6420(80)35127-0 10.1007/s10974-006-9067-4 |
ContentType | Journal Article |
Copyright | Copyright © 2013 by Korean Academy of Rehabilitation Medicine 2013 |
Copyright_xml | – notice: Copyright © 2013 by Korean Academy of Rehabilitation Medicine 2013 |
DBID | NPM AAYXX CITATION 5PM ACYCR |
DOI | 10.5535/arm.2013.37.2.157 |
DatabaseName | PubMed CrossRef PubMed Central (Full Participant titles) Korean Citation Index (Open Access) |
DatabaseTitle | PubMed CrossRef |
DatabaseTitleList | PubMed |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Physical Therapy |
EISSN | 2234-0653 |
EndPage | 166 |
ExternalDocumentID | oai_kci_go_kr_ARTI_826416 10_5535_arm_2013_37_2_157 23705109 |
Genre | Journal Article |
GroupedDBID | 5-W 8JR 8XY ABDBF ACYCR ADBBV ADRAZ AEGXH ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BCNDV DIK EF. GROUPED_DOAJ GX1 HYE KQ8 M48 NPM OK1 PGMZT RPM AAYXX CITATION 5PM |
ID | FETCH-LOGICAL-c454t-59f47988e0a3c4d20976d2e14f5ff88372888c8486fb9c88a76a4c2b60a5af0b3 |
IEDL.DBID | RPM |
ISSN | 2234-0645 |
IngestDate | Tue Nov 21 21:38:19 EST 2023 Tue Sep 17 21:16:33 EDT 2024 Fri Aug 23 02:26:23 EDT 2024 Sat Sep 28 08:09:09 EDT 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 2 |
Keywords | Botulinum toxins Skeletal muscle Proteomics Two-dimensional gel electrophoresis |
Language | English |
License | This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c454t-59f47988e0a3c4d20976d2e14f5ff88372888c8486fb9c88a76a4c2b60a5af0b3 |
Notes | G704-000430.2013.37.2.005 |
OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660475/ |
PMID | 23705109 |
PageCount | 10 |
ParticipantIDs | nrf_kci_oai_kci_go_kr_ARTI_826416 pubmedcentral_primary_oai_pubmedcentral_nih_gov_3660475 crossref_primary_10_5535_arm_2013_37_2_157 pubmed_primary_23705109 |
PublicationCentury | 2000 |
PublicationDate | 2013-04-01 |
PublicationDateYYYYMMDD | 2013-04-01 |
PublicationDate_xml | – month: 04 year: 2013 text: 2013-04-01 day: 01 |
PublicationDecade | 2010 |
PublicationPlace | Korea (South) |
PublicationPlace_xml | – name: Korea (South) |
PublicationTitle | Annals of rehabilitation medicine |
PublicationTitleAlternate | Ann Rehabil Med |
PublicationYear | 2013 |
Publisher | Korean Academy of Rehabilitation Medicine 대한재활의학회 |
Publisher_xml | – name: Korean Academy of Rehabilitation Medicine – name: 대한재활의학회 |
References | 2611094 - Br J Clin Pract. 1989 Nov;43(11):401-3 20733050 - J Cell Biol. 2010 Aug 23;190(4):491-500 11680887 - Proteomics. 2001 Mar;1(3):424-34 12015997 - J Chromatogr B Analyt Technol Biomed Life Sci. 2002 May 5;771(1-2):155-65 11987128 - Proteomics. 2002 May;2(5):543-50 7819880 - BMJ. 1994 Dec 10;309(6968):1526-7 7529693 - Electroencephalogr Clin Neurophysiol. 1994 Dec;93(6):434-9 3676924 - Can J Neurol Sci. 1987 Aug;14(3 Suppl):533-5 10429204 - Eur J Biochem. 1999 Jul;263(1):195-201 10077661 - Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):3200-5 15831715 - FASEB J. 2005 Jul;19(9):1143-5 7243198 - Ophthalmology. 1980 Oct;87(10):1044-9 6115105 - Med Biol. 1981 Feb;59(1):11-20 15917183 - Free Radic Biol Med. 2005 Jun 15;38(12):1543-52 10892733 - Electrophoresis. 2000 Jun;21(11):2228-34 16752196 - J Muscle Res Cell Motil. 2006;27(3-4):241-50 12069000 - J Speech Lang Hear Res. 2002 Jun;45(3):469-81 5119452 - J Neurol Sci. 1971 Sep;14(1):61-74 7876859 - J Neurol Neurosurg Psychiatry. 1995 Feb;58(2):232-5 942051 - Anal Biochem. 1976 May 7;72:248-54 18442174 - Proteomics. 2008 May;8(9):1933-44 16796804 - J Cell Mol Med. 2006 Apr-Jun;10(2):353-63 Isfort (10.5535/arm.2013.37.2.157_ref20) 2002; 2 de Paiva (10.5535/arm.2013.37.2.157_ref12) 1999; 96 Mishra (10.5535/arm.2013.37.2.157_ref21) 2006; 10 Sun (10.5535/arm.2013.37.2.157_ref17) 2006; 27 Welham (10.5535/arm.2013.37.2.157_ref9) 2008; 8 Sellin (10.5535/arm.2013.37.2.157_ref4) 1981; 59 Isfort (10.5535/arm.2013.37.2.157_ref8) 2002; 771 Ostergaard (10.5535/arm.2013.37.2.157_ref13) 1994; 93 Tsui (10.5535/arm.2013.37.2.157_ref1) 1987; 14 Scott (10.5535/arm.2013.37.2.157_ref2) 1980; 87 Walther (10.5535/arm.2013.37.2.157_ref14) 2010; 190 Neville (10.5535/arm.2013.37.2.157_ref7) 1994; 309 Piec (10.5535/arm.2013.37.2.157_ref16) 2005; 19 Nozais (10.5535/arm.2013.37.2.157_ref19) 1999; 263 Rhee (10.5535/arm.2013.37.2.157_ref22) 2005; 38 Boutsen (10.5535/arm.2013.37.2.157_ref3) 2002; 45 Bradford (10.5535/arm.2013.37.2.157_ref10) 1976; 72 Yan (10.5535/arm.2013.37.2.157_ref18) 2001; 1 Duchen (10.5535/arm.2013.37.2.157_ref11) 1971; 14 Das (10.5535/arm.2013.37.2.157_ref5) 1989; 43 Dunne (10.5535/arm.2013.37.2.157_ref6) 1995; 58 Isfort (10.5535/arm.2013.37.2.157_ref15) 2000; 21 |
References_xml | – volume: 72 start-page: 248 year: 1976 ident: 10.5535/arm.2013.37.2.157_ref10 publication-title: Anal Biochem doi: 10.1016/0003-2697(76)90527-3 contributor: fullname: Bradford – volume: 96 start-page: 3200 year: 1999 ident: 10.5535/arm.2013.37.2.157_ref12 publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.96.6.3200 contributor: fullname: de Paiva – volume: 263 start-page: 195 year: 1999 ident: 10.5535/arm.2013.37.2.157_ref19 publication-title: Eur J Biochem doi: 10.1046/j.1432-1327.1999.00486.x contributor: fullname: Nozais – volume: 58 start-page: 232 year: 1995 ident: 10.5535/arm.2013.37.2.157_ref6 publication-title: J Neurol Neurosurg Psychiatr doi: 10.1136/jnnp.58.2.232 contributor: fullname: Dunne – volume: 59 start-page: 11 year: 1981 ident: 10.5535/arm.2013.37.2.157_ref4 publication-title: Med Biol contributor: fullname: Sellin – volume: 190 start-page: 491 year: 2010 ident: 10.5535/arm.2013.37.2.157_ref14 publication-title: J Cell Biol doi: 10.1083/jcb.201004052 contributor: fullname: Walther – volume: 771 start-page: 155 year: 2002 ident: 10.5535/arm.2013.37.2.157_ref8 publication-title: J Chromatogr B Analyt Technol Biomed Life Sci doi: 10.1016/S1570-0232(02)00056-9 contributor: fullname: Isfort – volume: 8 start-page: 1933 year: 2008 ident: 10.5535/arm.2013.37.2.157_ref9 publication-title: Proteomics doi: 10.1002/pmic.200700859 contributor: fullname: Welham – volume: 14 start-page: 61 year: 1971 ident: 10.5535/arm.2013.37.2.157_ref11 publication-title: J Neurol Sci doi: 10.1016/0022-510X(71)90130-4 contributor: fullname: Duchen – volume: 93 start-page: 434 year: 1994 ident: 10.5535/arm.2013.37.2.157_ref13 publication-title: Electroencephalogr Clin Neurophysiol doi: 10.1016/0168-5597(94)90150-3 contributor: fullname: Ostergaard – volume: 10 start-page: 353 year: 2006 ident: 10.5535/arm.2013.37.2.157_ref21 publication-title: J Cell Mol Med doi: 10.1111/j.1582-4934.2006.tb00404.x contributor: fullname: Mishra – volume: 2 start-page: 543 year: 2002 ident: 10.5535/arm.2013.37.2.157_ref20 publication-title: Proteomics doi: 10.1002/1615-9861(200205)2:5<543::AID-PROT543>3.0.CO;2-K contributor: fullname: Isfort – volume: 19 start-page: 1143 year: 2005 ident: 10.5535/arm.2013.37.2.157_ref16 publication-title: FASEB J doi: 10.1096/fj.04-3084fje contributor: fullname: Piec – volume: 309 start-page: 1526 year: 1994 ident: 10.5535/arm.2013.37.2.157_ref7 publication-title: BMJ doi: 10.1136/bmj.309.6968.1526 contributor: fullname: Neville – volume: 21 start-page: 2228 year: 2000 ident: 10.5535/arm.2013.37.2.157_ref15 publication-title: Electrophoresis doi: 10.1002/1522-2683(20000601)21:11<2228::AID-ELPS2228>3.0.CO;2-V contributor: fullname: Isfort – volume: 38 start-page: 1543 year: 2005 ident: 10.5535/arm.2013.37.2.157_ref22 publication-title: Free Radic Biol Med doi: 10.1016/j.freeradbiomed.2005.02.026 contributor: fullname: Rhee – volume: 14 start-page: 533 issue: 3 Suppl year: 1987 ident: 10.5535/arm.2013.37.2.157_ref1 publication-title: Can J Neurol Sci doi: 10.1017/S0317167100038063 contributor: fullname: Tsui – volume: 45 start-page: 469 year: 2002 ident: 10.5535/arm.2013.37.2.157_ref3 publication-title: J Speech Lang Hear Res doi: 10.1044/1092-4388(2002/037) contributor: fullname: Boutsen – volume: 43 start-page: 401 year: 1989 ident: 10.5535/arm.2013.37.2.157_ref5 publication-title: Br J Clin Pract doi: 10.1111/j.1742-1241.1989.tb08783.x contributor: fullname: Das – volume: 1 start-page: 424 year: 2001 ident: 10.5535/arm.2013.37.2.157_ref18 publication-title: Proteomics doi: 10.1002/1615-9861(200103)1:3<424::AID-PROT424>3.0.CO;2-Y contributor: fullname: Yan – volume: 87 start-page: 1044 year: 1980 ident: 10.5535/arm.2013.37.2.157_ref2 publication-title: Ophthalmology doi: 10.1016/S0161-6420(80)35127-0 contributor: fullname: Scott – volume: 27 start-page: 241 year: 2006 ident: 10.5535/arm.2013.37.2.157_ref17 publication-title: J Muscle Res Cell Motil doi: 10.1007/s10974-006-9067-4 contributor: fullname: Sun |
SSID | ssj0000561921 |
Score | 1.9771029 |
Snippet | To observe the changes in protein expression induced by botulinum toxin A (BoNT-A) injection and to characterize the molecular and cellular action of... Objective To observe the changes in protein expression induced by botulinum toxin A (BoNT-A) injection and to characterize the molecular and cellular action of... |
SourceID | nrf pubmedcentral crossref pubmed |
SourceType | Open Website Open Access Repository Aggregation Database Index Database |
StartPage | 157 |
SubjectTerms | Original 재활의학 |
SummonAdditionalLinks | – databaseName: Scholars Portal Open Access Journals dbid: M48 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1LT9wwEB4BvfRS6HsLRUbqqVLSxI88Tmhb8ZS2RdWuxM2yvTHdUrw0yUrw75nZBMRKHDjlEGsifbYz35eMvwH44q3CrC5VVFjDI6lcEhVKqahUiVFVVqbO0XfI0c_seCJPz9X5Gty3t-oBbJ6UdtRPalL_i2_-3-7jhkf-Gisl1DdT05HyVMQij3mcqnwdXnApJC34Uc_2O6tvUgskwTAnyoic2rr_nE9HWclU66H2j5LUagHlo4x0uAWveirJht3cv4a1KryBzbMeeDbu_ALewq8z8mKg08esO0vQsFlgv03LjkzTYgIL1dVs0bDRosE4bEhdw9n3eUs16osrNp7f4PAhOwl_l2Vb4R1MDg_GP46jvo9C5KSSbaRKL8mWrEqMcHLKE6QgU16l0ivvC1SoHGWwK2SReVu6ojB5ZqTjNsPpMj6x4j1shHmoPgJD8uVFKtzUWiuRahljhLGJlW5aotBzA_h6j5m-7uwyNMoMAlgjwJoA1iLXXCPAA9hDVPWlm2kyuabrxVxf1hqp_IlG3YNkcQAfOqgfwnGR04ukHEC-MgkPAyjW6p0w-7M0zhZZlshcfXrGc7fhJV82vqAanR3YaOtF9RnpR2t3l4vqDgmm1uU priority: 102 providerName: Scholars Portal |
Title | Proteomic changes in rat gastrocnemius muscle after botulinum toxin a injection |
URI | https://www.ncbi.nlm.nih.gov/pubmed/23705109 https://pubmed.ncbi.nlm.nih.gov/PMC3660475 https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001763937 |
Volume | 37 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
ispartofPNX | Annals of Rehabilitation Medicine, 2013, 37(2), , pp.157-166 |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9swDBaannrZo90jexQa0NMAO7Yk-nHMivUxIF0wpEBugqRYq9tGKRIH2M8faSdFcu1FPlgmjE8EyM8mPzJ25i1gVFcQFdaISIFLogIAohISA1VWps7Rd8jRTXZ1q35NYXrAYNsL0xbtO1vH4XEeh_qura18mrvBtk5sMB6dyyxLVA6DHuvlUu5Q9E7QmzgBES2MfCoiPbbubyaABDRD7eepjGUeizgFGsInZE6uWe6Fpl5Y-p2otF8xuROCLt6wV5vckQ-7d3zLDqpwzF6PN0jzSScQcMJ-j0l8gdqNedc8sOJ14H9Mwy_NqsGIFap5vV7x0XqFdviQxoTzH4uGitLXcz5Z_MPtQ34d7ts6rfCO3V78nJxfRZvBCZFToJoISq9Ih6xKjHRqJhLMOWaiSpUH7wukpAJ5rytUkXlbuqIweWaUEzbD8zE-sfI9OwyLUH1kHLMtL1PpZtZahbmVMUYam1jlZiUyO9dn37eY6adOH0MjryCsNWKtCWstcy00Yt1n3xBV_eBqTarWdP270A9Ljbn7tUaig9lhn33ooH42tz2ePsv3DuF5A9nav4OO0yplbxzl04uf_MyORDv_gkp1vrDDZrmuvmIW0tjTlr3jejlNcR2p4rT1w_9v4N1D |
link.rule.ids | 230,315,733,786,790,870,891,2236,24346,27957,27958,53827,53829 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb9NAEB215QAXWr5KoIVF4oRkx94PfxxD1ZJAUyKUot5WuxsvmJBNldgS4tcza8dVwg1OPqw9svXWmnn2mzcAb60WmNW5CDKtaMCFiYJMCBHkIlKiSPLYGP8dcnyVDK_5xxtxswei64VpRPtGl6H7uQhd-b3RVt4uTL_TifUn4zOWJBFPRX8f7uH7StMtkt5aentW4KkW5j4eeEe29n-mEExgIN-AHrOQpSENY-HH8FGW-s2Z7ySnfbeyW3lpVzO5lYQuDuFrd_ut9mQe1pUOze-_nB3_-fmO4OGmLCWDdvkR7BXuMRxONiCSaes98AQ-T7yvg-9kJm1fwpqUjnxRFfmg1hUmQ1csynpNxvUa45CBn0BO3i8rr3evF2S6_IWnD8jI_WgkYO4pXF-cT8-GwWYmQ2C44FUgcsu9xVkRKWb4jEZYzsxoEXMrrM2Q7VKk1CbjWWJ1brJMpYnihuoEoVc20uwZHLilK54DwULOspiZmdaaY9mmlGJKR5qbWY6k0fTgXQeGvG2tNyRSFg-iRBClB1GyVFKJIPbgDcIl56aU3jDbH78t5XwlkRaMJHIoLDx7cNxieBeuw70H6Q66dyf4WLsriFljwr3B6MV_X_ka7g-n40t5Obr69BIe0GbMhlcEncBBtaqLUyx2Kv2q2dp_ANB1_Ho |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb9NAEF3RIiEutHwHWlgkTkh27P3wxzG0hAZIiVAqVVxWu2svmJBNlNgS4tczYydVwrEnH7weafXGmnn22zeEvHVGQlUXMsiMZoGQNgoyKWWQy0jLMslja_E75PgyubgSn67l9c6or1a0b00V-t_z0Fc_W23lcm77W51YfzI-40kSiVT2l4XrH5C78M6yfIeod7beyAyQbkH9EwG6snX_NKXkEoLhIfSYhzwNWRhLHMXHeIoJmu8VqAO_cju1aV83uVOIhkfk-3YLnf5kFja1Ce3f_9wdb7XHY_Jg057SQbfkIblT-kfkaLIBk047D4LH5OsE_R3wRDPtziesaeXpN13Tj3pdQ1H05bxq1nTcrCEOHeAkcvp-UaPuvZnT6eIPLB_Qkf_VSsH8E3I1_DA9uwg2sxkCK6SoA5k7gVZnZaS5FQWLoK0pWBkLJ53LgPUyoNY2E1niTG6zTKeJFpaZBFJAu8jwp-TQL3z5nFBo6ByPuS2MMQLaN6011yYywhY5kEfbI--2gKhlZ8GhgLogkAqAVAik4qliCoDskTcAmZrZSqFxNl5_LNRspYAejBRwKWhAe-RZh-NNuC32PZLuIXyzAGPt3wHcWjPuDU4vbv3ka3Jvcj5UX0aXn1-S-6ydtoHCoBNyWK-a8hR6ntq8arP7H8rm_vo |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Proteomic+Changes+in+Rat+Gastrocnemius+Muscle+After+Botulinum+Toxin+A+Injection&rft.jtitle=Annals+of+rehabilitation+medicine&rft.au=%ED%95%9C%EB%82%98%EB%AF%B8&rft.au=%EA%B9%80%ED%98%84%EB%8F%99&rft.au=%EC%97%84%EB%AF%B8%EC%9E%90&rft.au=Jun+Myeong+You&rft.date=2013-04-01&rft.pub=%EB%8C%80%ED%95%9C%EC%9E%AC%ED%99%9C%EC%9D%98%ED%95%99%ED%9A%8C&rft.issn=2234-0645&rft.eissn=2234-0653&rft.spage=157&rft.epage=166&rft_id=info:doi/10.5535%2Farm.2013.37.2.157&rft.externalDBID=n%2Fa&rft.externalDocID=oai_kci_go_kr_ARTI_826416 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2234-0645&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2234-0645&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2234-0645&client=summon |